161,383 results

1. pDestTol2-QUAS:GFP-CAAX

   Plasmid

   #184813

   Purpose

   Used to generate the Tg(QUAS:GFP-CAAX)c591 transgenic line

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   QUAS:GFP-CAAX-pA
   

   Use

   Zebrafish tol2 transgenesis

   Tags

   GFP-CAAX

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9-CBE6b-IVT

   Plasmid

   #215834

   Purpose

   Template for in vitro transcription of CBE6b (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

   Plasmid

   #205443

   Purpose

   human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD46-HLA-G SCT-hCD47-hCD59
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ES-FUCCI

   Plasmid

   #62451

   Purpose

   SMX_DEST vector with 3 cassettes

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Inserts

   minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
   minPGK::Cherry-hCdt1(30-120)-bGHpA
   PGK::HygroR-bGpA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpyTag003-MBP

   Plasmid

   #133450

   Purpose

   Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to N-terminus of MBP via a GSGE…

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. loSIIN

   Plasmid

   #185663

   Purpose

   shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

   Depositor

   Tyler Jacks

   Article

   Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

   Inserts

   mScarlet
   miR-30a
   shApc
   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAS162

   Plasmid

   #194485

   Purpose

   synGR Cassette

   Depositor

   Ahmad Khalil

   Article

   Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

   Insert

   synGR Cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOpen-P45

   Plasmid

   #165570

   Purpose

   Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   P45
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCBFD2Bad

   Plasmid

   #53574

   Purpose

   Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

   Insert

   cbfd2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMEH373 dTomato 3x high affinity MS2 loops

    Plasmid

    #168221

    Purpose

    Mammalian expression of dTomato with 3 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    dTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCVpuro-tdTomato

    Plasmid

    #97079

    Purpose

    Retroviral plasmid containing tdTomato for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-Duet1-His-TEV-mCherry-OPTN WT

    Plasmid

    #190191

    Purpose

    Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    6xHis, TEV, and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid

    #183411

    Purpose

    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    Tet-on 3G
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. UKNP1.21.5

    Plasmid

    #98203

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

    Plasmid

    #175526

    Purpose

    Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag11 and Tomm20-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187334

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTECH-MmAcK3RS(IPYE)

    Plasmid

    #104071

    Purpose

    Expression plasmid encoding MmAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp MmAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19/PLIN2-GFP

    Plasmid

    #202194

    Purpose

    knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP knock-in

    Expression

    Mutation

    Promoter

    lac

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEntry_HCoV-NL63-Spike

    Plasmid

    #168929

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-NL63-Spike (HCNV63gp2 )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tet-off Full length Tau (0N4R, P301L/V337M)

    Plasmid

    #188573

    Purpose

    Dox-repressible expression of the 0N4R Tau isoform

    Depositor

    Franz-Ulrich Hartl

    Article

    Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

    Insert

    0N4R Tau (MAPT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P301L and V337M

    Promoter

    pTight TRE

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEM mouse islet1 [TJ#28]

    Plasmid

    #16270

    Depositor

    Thomas Jessell

    Insert

    islet1 (Isl1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

22. His6TEV-UbG76C

    Plasmid

    #75299

    Purpose

    Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    human Ubiquitin mutant G76 to C
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Glycine 76 to Cystine

    Promoter

    T7

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGEX SH3(2)-4R

    Plasmid

    #112090

    Purpose

    Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-4R (NCK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pmSfrp2

    Plasmid

    #31249

    Depositor

    Jeremy Nathans

    Article

    Rattner et al Proc Natl Acad Sci U S A. 1997 Apr 1. 94

    Insert

    Sfrp2 (Sfrp2 Mouse)
    

    Use

    Cdna for in situ hybridization

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pRK5-A3-HA-ubiquitin

    Plasmid

    #220145

    Purpose

    Expresses A3-Ub for E3 substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    A3-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pFTK055

    Plasmid

    #171327

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    rTA2S-M2 (TetR-3xVP16) transcriptional activator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV_UdgX-EE-UdgX-nCas9-UdgX

    Plasmid

    #163566

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9-UdgX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PB-SAM

    Plasmid

    #102559

    Purpose

    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Inserts

    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9 and N55K in MS2

    Promoter

    CAG and EF1A

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. EGFP-HsNRF2 (NFE2L2)

    Plasmid

    #194303

    Purpose

    Mammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJet1.2 ermB cassette

    Plasmid

    #117121

    Purpose

    Erythromycin resistance cassette

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Erythromycin resistance cassette for Bacillus subtilis selection
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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