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pET24a His6 GST PYL2(14-188)

Plasmid

#177842

Purpose

Bacterial Expression of PYL2

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 2 (PYL2 Mustard Weed)

Tags

6xHis-GST

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-Puro-PXN

Plasmid

#227317

Purpose

Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

PXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2 GFP-Rtn4a

Plasmid

#86684

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4a (RTN4 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pN1-PinkyCaMP

Plasmid

#232861

Purpose

A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

Depositor

Olivia Masseck

Article

Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

Insert

PinkyCaMP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pX601-GFP

Plasmid

#84040

Purpose

Staphylococcus aureus (SaCas9) conjugated with GFP

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Integrin β3-GFP

Plasmid

#205091

Purpose

Express GFP-tagged human integrin β3 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 3 (ITGB3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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NES-PAmCherry-cPHx2

Plasmid

#116860

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

PAmCherry

Expression

Mammalian

Mutation

amino acids 169-329 (tandem dimer)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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p2R3a-nosT

Plasmid

#71272

Purpose

Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

nopaline synthase terminator

Use

Gateway

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pEvol-pAzFRS.1.t1

Plasmid

#73547

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.1.t1
pAzFRS.1.t1

Expression

Bacterial

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pSI-Target-AID-NG

Plasmid

#119861

Purpose

Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

Depositor

Osamu Nureki

Article

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFB2C

Plasmid

#195285

Purpose

Bicistronic HSV-TK and Hygromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A Hygro

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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p1.2-GS

Plasmid

#162770

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

Type

Empty backbone

Expression

Mammalian

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin

Plasmid

#134869

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

cytochrome b5 type A (CYB5A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-98

Promoter

EF1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-IgK-R-eLACCO2.1-COBRA

Plasmid

#223259

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

R-eLACCO2.1

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-IgK-R-eLACCO2-COBRA

Plasmid

#223258

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

R-eLACCO2

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-IgK-R-deLACCOctrl-COBRA

Plasmid

#223260

Purpose

Control biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

R-deLACCOctrl

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEvol-pAzFRS.2.t1

Plasmid

#73546

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.2.t1
pAzFRS.2.t1

Expression

Bacterial

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-CCO-IRES-Puromycin

Plasmid

#134861

Purpose

Expresses EGFP-tagged inner mitochondrial membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

cytochrome c oxidase subunit VIII (COX8A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_AnapRS

Plasmid

#174894

Purpose

AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AnapRS

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pB-TAG-NICD

Plasmid

#130934

Purpose

PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NICD (NOTCH1 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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