We narrowed to 26,580 results for: nans
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Plasmid#72928PurposeAllows expression of a modified version of the fission yeast NatA complex - chloramphenicol markerDepositorExpressionBacterialMutationCodon optomised for E.coli expressionPromoterT7Available SinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only
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pLenti-HRE-dUnaG
Plasmid#124372PurposeUnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signalingDepositorInsertHRE-dUnaG
UseLentiviralTagsPEST-degron and Myc tagPromoterHRE (HIF responsive element 5x + CMV minimal prom…Available SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
NES-PAmCherry-cPHx2
Plasmid#116860PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
TagsPAmCherryExpressionMammalianMutationamino acids 169-329 (tandem dimer)Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
BMR1_03g02090-bio-His
Plasmid#107674PurposeExpresses enzymatically monobiotinylated full-length BMR1_03g02090 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertBMR1_03g02090
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-hMLH1dn for IVT
Plasmid#178114PurposeTemplate for in vitro transcription of human MLH1dnDepositorInserthuman MLH1dn (codon optimized) (MLH1 Human)
UseTemplate for in vitro transcriptionMutationDetailed in manuscriptPromoterT7 (inactivated)Available SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-2
Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailable SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.1.t1
Plasmid#73547PurposetRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.1.t1
pAzFRS.1.t1
ExpressionBacterialAvailable SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Cre
Plasmid#55636PurposeExpresses Cre in Mammalian CellsDepositorHas ServiceAAV Retrograde and AAV11InsertCre
UseAAV and Cre/LoxExpressionMammalianPromoterEf1aAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-Egfr(Velvet)
Plasmid#18788DepositorInsertepidermal growth factor receptor (Egfr Mouse)
TagsFLAGExpressionMammalianMutationD833G; signal sequence removed (aa1-24)Available SinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flpo
Plasmid#55637PurposeExpresses Flpo in Mammalian CellsDepositorHas ServiceAAV Retrograde, AAV1, and AAV2InsertFlpo
UseAAVExpressionMammalianPromoterEf1aAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.2.t1
Plasmid#73546PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.2.t1
pAzFRS.2.t1
ExpressionBacterialAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-NLS-TIR1_P2A_NES-TIR1
Plasmid#117699PurposeExpression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNADepositorInsertsTRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1
TagsHA tag, Myc-NES, and NLSExpressionMammalianMutationcodon optimized for murine expressionPromoterNone, 2A peptide from porcine teschovirus-1 polyp…Available SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.tRFP
Plasmid#57826PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP
UseCRISPR and LentiviralTagsFLAGAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCI-His-hUbi
Plasmid#31815DepositorAvailable SinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG_PSD95.FingR-eGFP-CCR5TC
Plasmid#46295DepositorAvailable SinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-SPOTlight-U2GCR
Plasmid#164819PurposeReporter to measure ISR state-dependent protein synthesisDepositorInsertSPOTlight-U2GCR
UseAAVExpressionMammalianPromoterCAGAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBF_HsPepT2
Plasmid#73944PurposeXenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tagDepositorInsertPepT2 (SLC15A2 Human)
UseMake rna for xenopus oocyte injectionTagsFlagPromoterbeta globinAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBF_HsPepT1
Plasmid#73943PurposeXenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tagDepositorInsertPepT1 (SLC15A1 Human)
UseMake rna for xenopus oocyte injectionTagsFlagPromoterbeta globinAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
KIF1A(1-393)-GCN4-2xmCherry-EF(C)
Plasmid#61665PurposeTandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linkerDepositorInsertKIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)
ExpressionMammalianAvailable SinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…Available SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only