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pMLM36 (aka pBR-UV5-GP-FD2)

Plasmid

#21871

Depositor

Keith Joung

Publication

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCVL SFFV-EF1s HA.NLS.Sce(opt)

Plasmid

#31479

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV/Ef1s HA NLS I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

"opt" indicates the coding sequence has…

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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scFLARE2

Plasmid

#158700

Purpose

Neuronal expression of scFLARE2 component

Depositor

Alice Ting

Publication

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE2

Use

AAV

Tags

HA, Flag, VP16

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

Syn

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pXG260

Plasmid

#141282

Purpose

CMV:EGFP (as control for pXG237)

Depositor

Martin Kampmann

Publication

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

EGFP

Use

Lentiviral

Promoter

CMV

Available since

Sept. 19, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-PR rep/cap

Plasmid

#197565

Purpose

encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

Depositor

Casey Maguire

Publication

Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

Inserts

AAV Rep genes
AAV9 VP1 Cap gene with PR insert

Use

AAV

Mutation

contains 7mer insert PRPPSTH between amino acids …

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIMAY*

Plasmid

#121441

Purpose

Allelic exchange plasmid with PheS* counter-selection for Staphylococcus aureus

Depositor

Angelika Grundling

Publication

Schuster et al Microbiology. 2019 Apr 3. doi: 10.1099/m

Type

Empty backbone

Use

Escherichia coli/staphylococcus aureus shuttle ve…

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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LcV2-Hygro

Plasmid

#91977

Purpose

LentiCRISPR v2 with Hygro cloned in place of Puro

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pcDNA3-HA PTEN

Plasmid

#78776

Purpose

To overexpress PTEN in Mammalian Cells

Depositor

Jaewhan Song

Publication

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV-Fos-CreERT2

Plasmid

#194643

Purpose

Expresses Cre-ERT2 under the control of c-Fos promoter

Depositor

Jun-Hyeong Cho

Publication

Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

Insert

Fos-CreERT2

Use

AAV

Expression

Mammalian

Promoter

c-Fos

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EGFP-BAF

Plasmid

#101772

Purpose

Expresses EGFP tagged BAF in human cells

Depositor

Daniel Gerlich

Publication

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGEX-GST-AGO2

Plasmid

#24317

Depositor

Carl Novina

Publication

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

human AGO2 (AGO2 Human)

Tags

GST

Expression

Bacterial

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pEIGW roGFP2-Orp1

Plasmid

#64993

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

Depositor

Tobias Dick

Publication

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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HPV16 E7 TCR

Plasmid

#122728

Purpose

Express HPV16 E7 TCR

Depositor

Christian Hinrichs

Publication

Jin et al JCI Insight. 2018 Apr 19;3(8). pii: 9948

Insert

HPV16 E7 TCR

Use

Retroviral

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV-MbPylRS(DiZPK)

Plasmid

#91706

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

Depositor

P. Chen

Publication

Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

Inserts

pylS
pylT

Expression

Mammalian

Mutation

L274A, C313S

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Cas9-2A-Cre

Plasmid

#68468

Purpose

co-expressing Cas9 and Cre recombinase

Depositor

Su-Chun Zhang

Publication

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 and Cre recombinase

Use

CRISPR

Tags

P2A-Cre-Myc

Expression

Mammalian

Promoter

CAG

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Expression

Mammalian

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shp53/GFP4

Plasmid

#20208

Depositor

John Ohlfest

Publication

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

shp53/GFP4

Use

Transposon

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pPEgRNA

Plasmid

#172716

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Publication

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

gRNA

Expression

Bacterial

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pESC-leu2d(empty)

Plasmid

#20120

Depositor

Jay Keasling

Publication

Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCas3cRh

Plasmid

#133773

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Joseph Bondy-Denomy

Publication

Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8
Cas7

Expression

Bacterial

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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