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Plasmid#52154PurposeEncodes module 4 with amino acids 12S-16R for recognition of nt 5CDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 4PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4CYQ
Plasmid#52155PurposeEncodes module 4 with amino acids 12C-13Y-16Q for recognition of nt 5ADepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C and 13H to 13Y in Module 4PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4NYQ
Plasmid#52157PurposeEncodes module 4 with amino acids 12N-13Y-16Q for recognition of nt 5UDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 13H to 13Y in Module 4PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5SE
Plasmid#52160PurposeEncodes module 5 with amino acids 12S-16E for recognition of nt 4GDepositorInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12C to 12S and 16Q to 16E in Module 5PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5NQ
Plasmid#52161PurposeEncodes module 5 with amino acids 12N-16Q for recognition of nt 4UDepositorInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12C to 12N in Module 5PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-6CQ
Plasmid#52163PurposeEncodes module 6 with amino acids 12C-16Q for recognition of nt 3ADepositorInsertPumilio homology domain amino acids 205-240 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C in Module 6PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-6SR
Plasmid#52166PurposeEncodes module 6 with amino acids 12S-16R for recognition of nt 3CDepositorInsertPumilio homology domain amino acids 205-240 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 6PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pY128
Plasmid#127713PurposeLevel 1 yeast shuttle vector for a transcription unit with A - D scars. Clone in with BsaI. Has a high-copy yeast replicon and KanMX selection.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-CrkII
Plasmid#50024PurposepENTR vector for mouse Crk-IIDepositorInsertCrk-II (Crk Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-TEF2p-LDH-CYC1t
Plasmid#216120PurposeYeast integrative vector for constitutive expression of LDH at the LEU2 locusDepositorInsertLactate dehydrogenase (LDH Bovine)
UseTagsExpressionYeastMutationPromoterTEF2 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-LDH-CYC1t
Plasmid#216121PurposeYeast integrative vector for constitutive expression of LDH at the TRP1 locusDepositorInsertLactate dehydrogenase (LDH Bovine, Bos taurus)
UseTagsExpressionYeastMutationPromoterTDH3 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-CDC19-CYC1t
Plasmid#216122PurposeYeast integrative vector for constitutive expression of CDC19 at the TRP1 locusDepositorInsertPyruvate kinase 1
UseTagsExpressionYeastMutationPromoterTDH3 promoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-GFPenhancer
Plasmid#202468PurposeExpresses the nanobody GFP enhancer after phosphate depletionDepositorInsertnanobody GFP enhancer
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-GFPminimizer
Plasmid#202469PurposeExpresses the nanobody GFP minimizer after phosphate depletionDepositorInsertnanobody GFP minimizer
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-H6antivenin
Plasmid#202470PurposeExpresses the nanobody H6 antivenin after phosphate depletionDepositorInsertnanobody H6 antivenin
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-1B5
Plasmid#202472PurposeExpresses the nanobody 1B5 after phosphate depletionDepositorInsertnanobody 1B5
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-2E7
Plasmid#202473PurposeExpresses the nanobody 2E7 after phosphate depletionDepositorInsertnanobody 2E7
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-3E3
Plasmid#202474PurposeExpresses the nanobody 3E3 after phosphate depletionDepositorInsertnanobody 3E3
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-C9BoNT-A
Plasmid#202475PurposeExpresses the nanobody C9 BoNT-A after phosphate depletionDepositorInsertnanobody C9 BoNT/A
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-E7BoNT-A
Plasmid#202476PurposeExpresses the nanobody E7 BoNT-A after phosphate depletionDepositorInsertnanobody E7 BoNT/A
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only