We narrowed to 34,664 results for: cas

1. pAW91.dCas9

   Plasmid

   #104372

   Purpose

   Expresses dCas9

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Go1-CASE

   Plasmid

   #168123

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG9

   Expression

   Mammalian

   Mutation

   NLuc is inserted at L91/G92 within GNAO1

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. dead Sa Cas9

   Plasmid

   #138162

   Purpose

   For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   dSaCas9
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D10A + N580A

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9_T2A_mCherry sgBFP

   Plasmid

   #192929

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCas9-GFP

   Plasmid

   #78546

   Purpose

   Fusion protein Cas9+GFP

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Fusion protein with Cas9

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9(D10A)

   Plasmid

   #74495

   Purpose

   Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

   Insert

   Cas9 D10A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   D10A

   Promoter

   pTeto

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459_gRNA-ROSA26_hspCas9

   Plasmid

   #193310

   Purpose

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC1801_pmax-dCasRx

   Plasmid

   #118634

   Purpose

   Transient transfection; Expresses dCasRx; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   dCasRx
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R239A/H244A/R858A/H863A

   Promoter

   CAGGS

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. nos-Cas9.874Z1

   Plasmid

   #112685

   Purpose

   Express hSpCas9 under nanos promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Use

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   nanos

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9 TadDE

    Plasmid

    #193837

    Purpose

    Express TadDE (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-DualEditor-Cas9D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. G15-CASE

    Plasmid

    #168126

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at E244/N245 within GNA15

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-dMSK1

    Plasmid

    #165602

    Purpose

    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 S. Pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ezh2[FL]-dCas9

    Plasmid

    #100086

    Purpose

    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    14056 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Ezh2[FL]-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nEFCas9

    Plasmid

    #87115

    Purpose

    SpCas9 expression AAV backbone plasmid under control of nEF promoter

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    nEF-Cas9
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-Cas9

    Plasmid

    #89995

    Purpose

    Expresses high levels of WT Cas9 for genome editing

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

    Insert

    hCas9
    

    Use

    Tags

    3xFlag, SV40 NLS, and T2A peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. mARG1 cassette 3

    Plasmid

    #134345

    Purpose

    Mammalian acoustic reporter gene 1 cassette 3 on the piggyBac trasposon plasmid.

    Depositor

    Mikhail Shapiro

    Article

    Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

    Insert

    mARG 1 cassete 3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME-Cas9

    Plasmid

    #63154

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAEL010097-Cas9

    Plasmid

    #100707

    Purpose

    The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL010097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL010097 and Opie2

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. ALKBH5-dCas9

    Plasmid

    #134783

    Purpose

    Expression fusion protein ALKBH5-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    ALKBH5 and inactive dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pColdI-DDX3_helicase_core

    Plasmid

    #233618

    Purpose

    Expression of His-tagged helicase core region of DDX3X in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    DDX3X helicase core WT (DDX3X Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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