Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 60,551 results for: Gene

Clear keywords
Showing: 11311 - 11340 of 60551 results

  1. p426_Cas9_gRNA-ARS308a

    Plasmid
    #87384
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS308a sequence CACTTGTCAAACAGAATATA in yeast chromosome 3
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS308a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87384-plasmid-map-sequence-id-193426

    Enlarge

  9. pINER5

    Plasmid
    #185895
    Purpose
    In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Article
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
    Expression
    Yeast
    Mutation
    None
    Available Since
    Aug. 25, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185895-plasmid-map-sequence-id-370388

    Enlarge

  12. pLY61

    Plasmid
    #130928
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130928-plasmid-map-sequence-id-257360

    Enlarge

  15. pZH519

    Plasmid
    #102672
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102672-plasmid-map-sequence-id-199600

    Enlarge

  16. pZH522

    Plasmid
    #102675
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102675-plasmid-map-sequence-id-199391

    Enlarge

  17. pPMVAd36

    Plasmid
    #98302
    Purpose
    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.
    Depositor
    Claudia Vickers
    Article
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1
    Expression
    Yeast
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    98302-plasmid-map-sequence-id-190207

    Enlarge

  20. pBALU12

    Plasmid
    #69328
    Purpose
    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd mChO intron;use C-term after STOP
    Depositor
    Oliver Hobert
    Article
    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/
    Insert
    gSL2-NLS-mChOint-FgF
    Expression
    Bacterial
    Available Since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. SNV-gfp-IRES-MCS

    Plasmid
    #53122
    Purpose
    Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells
    Depositor
    Jeanette Hyer
    Article
    Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1
    Type
    Empty backbone
    Use
    Retroviral; For expression in chicken cells
    Tags
    EGFP-IRES
    Promoter
    SNV (spleen necrosis virus)
    Available Since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    53122-plasmid-map-sequence-id-82652

    Enlarge

  27. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

    Plasmid
    #188385
    Purpose
    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    Use
    Lentiviral
    Available Since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188385-plasmid-map-sequence-id-371050

    Enlarge

Showing: 11311 - 11340 of 60551 results