161,424 results

1. pQTEV-PTD004

   Plasmid

   #34798

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   GTP-binding protein PTD004 (OLA1 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. M7 pdnPKA-GFP

   Plasmid

   #16716

   Depositor

   Randall Moon

   Article

   Ungar et al Dev Biol. 1996 Aug 25;178(1):186-91. doi

   Insert

   dnPKA (Prkar1a Mouse)
   

   Use

   Xenopus expression

   Tags

   GFP

   Expression

   Mutation

   Dominant negative. Mutations at cAMP binding site…

   Promoter

   Available since

   April 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_hSyn-PdCO-EGFP-WPRE

   Plasmid

   #198513

   Purpose

   Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV

   Tags

   EGFP and Rho1D4

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG/ATP9A(E195Q)-HA

   Plasmid

   #209244

   Purpose

   Mammalian expression of ATP9A mutant E195Q

   Depositor

   Hye-Won Shin

   Article

   Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

   Insert

   ATP9A (ATP9A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E195Q

   Promoter

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-GRAM-H

   Plasmid

   #211706

   Purpose

   Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H) in mammalian cells

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Tags

   ddFP-B3

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Leucine

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-dCas9-VP64-BFP

   Plasmid

   #196711

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-VP64-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBXNH3CA_MBP

   Plasmid

   #132700

   Purpose

   expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Maltose binding Protein
   

   Use

   Tags

   Avi and His

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

   Plasmid

   #154827

   Purpose

   Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   TagBFP
   HygR
   ZsGreen1-DR
   

   Use

   CRISPR and Cre/Lox

   Tags

   PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, EF1a, and SV40 early promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHSB04X

   Plasmid

   #117258

   Purpose

   Genome editing for Lactobacillus brevis ATCC367

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PTPRC-bio-His

    Plasmid

    #51813

    Purpose

    Expresses full-length Leukocyte common antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PTPRC (PTPRC Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-flag-hKDM4A(H188A)-polyA

    Plasmid

    #101052

    Purpose

    in vitro transcription (T7 promoter)

    Depositor

    Yi Zhang

    Article

    Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

    Insert

    KDM4A (KDM4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-mito-CitronRH

    Plasmid

    #158547

    Purpose

    Mitochondria-targeted nonbinding variant of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitronRH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV5B-ALK4

    Plasmid

    #11756

    Depositor

    Jeff Wrana

    Article

    Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

    Insert

    ALK4 (ACVR1B Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

14. mCherry-Calnexin-N-14

    Plasmid

    #55005

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Calnexin (CANX Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    I474N in Calnexin

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. LeGO-V2

    Plasmid

    #27340

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, Venus fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUB-GFP

    Plasmid

    #11155

    Purpose

    Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    Ubiquitin C promoter (UBC Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO GFP

    Plasmid

    #19444

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRX-IZ-ANXA6-mRuby3

    Plasmid

    #184892

    Purpose

    Stable expression of ANXA6-mRuby3 (Annexin A6-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA6 (ANXA6 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    R587K

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEnt L1L3 EF1a-tTA-2

    Plasmid

    #26803

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    Elongation Factor 1 alpha promoter and tTA transgene
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOZ-N-FH RAP80

    Plasmid

    #27501

    Depositor

    Roger Greenberg

    Article

    Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

    Insert

    RAP80 (UIMC1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMLRT

    Plasmid

    #78234

    Purpose

    Expresses Murine Leukemia Reverse Transcriptase (MLRT)

    Depositor

    Christopher Voigt

    Article

    Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

    Insert

    Murine leukemia reverse transcriptase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23102

    Available since

    Aug. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. MSCV-HA tagged-Mouse Mdm2 wt-IRES-GFP

    Plasmid

    #180491

    Purpose

    Role of Mdm2 splicing variants in tumorigenesis

    Depositor

    Martine Roussel

    Article

    Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

    Insert

    mouse Mdm2 full length (wt)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLKO.1 - TRC mTagBFP2

    Plasmid

    #191566

    Purpose

    Expresses an shRNA and mTagBFP2

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

    Plasmid

    #217340

    Purpose

    crRNA array targeting CD81, B2M, KIT, CD55

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ARTX_ctrl

    Plasmid

    #214065

    Purpose

    Fluorescent reporter system, NMD negative control. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Remnant of linker sequence present at the end of …

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV2-ProC3-Cre/mCherry

    Plasmid

    #126006

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    Cre-2A-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC3

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. Cytosolic FAK biosensor

    Plasmid

    #78300

    Purpose

    FRET biosensor. Visualization of cytosolic FAK activity in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Cytosolic FAK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AbVec2.0-mIghg1

    Plasmid

    #127158

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg1 (Ighg1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCCLc-MND-A0201-Mart1-SABR

    Plasmid

    #119052

    Purpose

    3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Insert

    A0201-Mart1-SABR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MND

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKS071 - pCAGGS-3XFLAG-mKate2-(human)PDS5A-bpA

    Plasmid

    #156449

    Purpose

    For transient expression of human PDS5A tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    human PDS5A (PDS5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge