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We narrowed to 34,868 results for: CaS

Showing: 741 - 760 of 34868 results

  1. pYLCRISPR/Cas9P35S-N

    Plasmid
    #66191
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection
    Depositor
    Yao-Guang Liu
    Article
    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(
    Insert
    Cas9
    Use
    Tags
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62…
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66191-plasmid-map-sequence-id-404047

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  2. pK7WGF2::hCas9

    Plasmid
    #46965
    Purpose
    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue
    Depositor
    Sophien Kamoun
    Article
    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.
    Insert
    hCas9
    Use
    CRISPR; Plant expression
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    35S
    Available since
    Aug. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    46965-plasmid-map-sequence-id-66887

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  5. gGBEv6.3-SpCas9

    Plasmid
    #202629
    Purpose
    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.
    Insert
    nSpCas9(D10A)-MPGv6.3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…
    Promoter
    hU6, EF1a, CBh
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202629-plasmid-map-sequence-id-398425

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  7. pKS diaCas9_sgRNA

    Plasmid
    #74923
    Purpose
    Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter
    Depositor
    Per Winge
    Article
    Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Cas9 module and sgRNA module
    Available since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74923-plasmid-map-sequence-id-149703

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  10. pCS2+ heiCas9

    Plasmid
    #186929
    Purpose
    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS
    Depositor
    Joachim Wittbrodt
    Article
    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:
    Insert
    myc-flexible linker-oNLS-Cas9-oNLS
    Use
    CRISPR
    Tags
    myc-flexible linker-optimizedNLS and optimizedNLS
    Expression
    Mammalian
    Mutation
    Promoter
    sp6
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    186929-plasmid-map-sequence-id-390733

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  12. pLKO5d.SFFV.Cas9.P2A.BSD

    Plasmid
    #181981
    Purpose
    Lentiviral Cas9 expression
    Depositor
    Jan-Henning Klusmann
    Insert
    Cas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    181981-plasmid-map-sequence-id-366611

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  13. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

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  15. CASR-Tango

    Plasmid
    #66235
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CASR (CASR Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66235-plasmid-map-sequence-id-378710

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  16. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Article
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

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  17. dCas12Max-CBE

    Plasmid
    #195337
    Purpose
    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-CBE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195337-plasmid-map-sequence-id-383131

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Showing: 741 - 760 of 34868 results