165,572 results
-
Plasmid#141286PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only
-
EGFP-Tau(T217A)
Plasmid#187025PurposeExpresses EGFP tagged human 2N4R tau (with T217A mutation) in mammalian cells with CMV promoterDepositorInsertMAPT (MAPT Human)
UseTagsEGFPExpressionMammalianMutationChanged Threonine 217 to AlaninePromoterCMVAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_S5E2_Chrimson_tdTomato
Plasmid#219432PurposeAAV construct expressing Chrimson-tdTomato under the control of E2 regulatory elementDepositorInsertChrimson-tdTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA3-Flag-IDH1
Plasmid#62906PurposeExpresses wildtype IDH1 in mammalian cellsDepositorInsertisocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Shen Lab DdCBE assembly kit
Plasmid Kit#1000000212PurposeThe DddA-derived cytosine base editor (DdCBE) assembly kit is a collection of 192 modules harboring repeat variable di-residue (RVD) arrays for any desired target.DepositorAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Golgi-7
Plasmid#58018PurposeLocalization: Golgi Complex, Excitation: 555, Emission: 584DepositorInsertGolgi (B4GALT1 Human)
UseTagsmTagRFPExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
AiP1051-pAAV-Synapsin promoter-oNigri-WPRE-hGHpA
Plasmid#163489PurposeDirect-expressing oNigri AAV VirusHas ServiceAAV1InsertoNigri
UseAAVTagsExpressionMutationPromoterSynapsin promoterAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
2C::tdTomato Reporter
Plasmid#40281DepositorInsert2C Promoter (2-cell stage promoter)
UseTagstdTomatoExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTK168_mCherry-H2B
Plasmid#46363Purposevisualizes chromosomesDepositorInsertH2B (H2BC11 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationD26G, V119I and S125DPromoterpBABEAvailable sinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-mPIAS1
Plasmid#15206DepositorInsertPIAS1 (Pias1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
856 pBabe puroL PTEN C124S
Plasmid#10931DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutationC124SPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-JEDI-2P-Kv-WPRE (AAV9)
Viral Prep#179463-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-JEDI-2P-Kv-WPRE (#179463). In addition to the viral particles, you will also receive purified pAAV-hSyn-JEDI-2P-Kv-WPRE plasmid DNA. hSyn-driven expression (soma and AIS-localized) of the genetically encoded voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-TRAC-miniGag
Plasmid#228960PurposeminiEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet
Plasmid#162280PurposeEukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN8A Variant 1, stabilized (SCN8A Human)
UseTagsIRES-mScarletExpressionMammalianMutationModified human HSPA5 intron 4 inserted after bp23β¦PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5/DEST-VPS35
Plasmid#21691DepositorInsertVacuolar Protein Sorting 35 homolog (VPS35 Human)
UseLentiviral; Invitrogen gateway destination vectorTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA
Plasmid#196192Purposehuman synapsin promoter driven dTomato expression at human AAVS1 safe harbour locusDepositorInsert2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
UseZnf donorTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC)
Plasmid Kit#1000000175PurposePlasmids and entry vectors for diverse fluorescent sensors of physiological parameters in cells.DepositorAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-uvsY
Plasmid#163914PurposeExpresses uvsY for bacterial expression and affinity purificationDepositorInsertuvsY (uvsY Enterobacteria phage T4)
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
hFLT1
Plasmid#83435Purposehuman VEGFR1 with HA and FLAG tagDepositorInserthuman VEGFR1 (FLT1 Human)
UseTagsFlag and MycExpressionMammalianMutationHA-tag was inserted 30 AA downstream of the FLT1 β¦PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2ST
Plasmid#59384PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.DepositorInsertTT-ISceI-dfrA
UseTemplateTagsExpressionMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_JN.1
Plasmid#218678Purposeexpressing SARS-CoV-2 JN.1 spike protein for pseudovirus productionDepositorInsertSpike (JN.1)
UseTagsExpressionMammalianMutationL455S on the basis of BA.2.86PromoterCMVAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002
Plasmid#102827PurposeBacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteinsDepositorInsertSpyCatcher002
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-TVA-oG-mCherry
Plasmid#166602PurposeEncodes Cre-dependent TVA-oG-mCherry under control of the TREDepositorInsertTVA-oG-mCherry
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-dCas9-EGFP-HaloTag
Plasmid#169451Purposeexpression of dCas9-EGFP-HaloTagDepositorInsertdCas9-EGFP-HaloTag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mhYFP
Plasmid#186532PurposeMonomeric hyperfolder YFP fluorescent protein: bacterial expression plasmidDepositorInsertMonomeric hyperfolder YFP
UseTags6xHisExpressionBacterialMutationhfYFP-S147P/L195M/V206KPromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G
Plasmid#194457PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ΞΆ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
RelA cFlag pcDNA3
Plasmid#20012DepositorInsertRelA (Rela Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
MscL(G22S)61-cpGFP-ERexp
Plasmid#195311PurposeFluorescent reporter/biosensor for membrane tensionDepositorInsertMscL(G22S)61-cpGFP-ERexp
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc
Plasmid#117728PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc geneDepositorInsertNanoLuc
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only