161,473 results

1. R777-E321 Hs.SPRED1

   Plasmid

   #70605

   Purpose

   Gateway ORF clone of human SPRED1 [NM_152594.2] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-PEmax-SpG

   Plasmid

   #211549

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

   Insert

   PEmax-SpG
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmTurquoise2-Tubulin

   Plasmid

   #36202

   Purpose

   In vivo visualization of microtubules (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   Alpha-tubulin (TUBA1B Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. EcSTH-LbNOX

   Plasmid

   #211924

   Purpose

   This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.

   Depositor

   Binghui Li

   Article

   Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

   Insert

   EcSTH-LbNOX
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5E-kdrl

   Plasmid

   #78687

   Purpose

   kdrl promoter for Gateway cloning

   Depositor

   Jau-Nian Chen, Sarah Childs

   Article

   Choi et al Dev Biol. 2007 Apr 15;304(2):735-44. Epu

   Insert

   kdrl promoter (kdrl Zebrafish)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   kdrl

   Available since

   June 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKanCMV-mClover3-mRuby3

   Plasmid

   #74252

   Purpose

   Mammalian expression of tandem fusion of mClover3-mRuby3

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   mClover3-mRuby3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTECH-chPylRS(IPYE)

   Plasmid

   #99222

   Purpose

   Expression plasmid encoding chPylRS(IPYE) and pylT

   Depositor

   Dieter Söll

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp chPyl(IPYE)RS; PproK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Anti-GFAP [N206A/8R] (Recombinant Antibody)

   Antibody

   #193445-rAb

   Purpose

   Anti-GFAP (Human) recombinant mouse monoclonal antibody

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Recommended Applications

   Immunohistochemistry and Western Blot

   Reactivity

   Human, Mouse, and Rat

   Source Species

   Mouse

   Isotype

   IgG2a

   Trial Size

   Available to purchase

   Available since

   Jan. 30, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

9. pHL-avitag3

   Plasmid

   #99847

   Purpose

   mammalian expression with secretive signal and avi tag for biotinylation

   Depositor

   Edith Yvonne Jones

   Article

   Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   ephrin b2 (EPHB2 Human)
   

   Use

   Tags

   Avi

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPUR-hU6-sgRNA-Sirius-8XMS2

    Plasmid

    #121942

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. HiBiT-FZD2

    Plasmid

    #195840

    Purpose

    Expresses human FZD2 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD2 (FZD2 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBKT7-GW

    Plasmid

    #61703

    Purpose

    Gateway compatible Y2H vector - BD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDAC627

    Plasmid

    #195240

    Purpose

    Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid

    #217342

    Purpose

    crRNA array targeting CD81, B2M, KIT, CD55

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-FKBP-SAC1deltaTMD

    Plasmid

    #108132

    Purpose

    Recruitable cytosolic SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-521)

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17

    Plasmid

    #210020

    Purpose

    tetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Klf4-IRES-Sox2-17 (Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-mini-CMV

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMal-T-Avi-His/BirA

    Plasmid

    #102962

    Purpose

    Vector for in vivo biotinylation of a fusion protein in bacteria.

    Depositor

    Tonia Rex

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS mNotch1-IC (DM#270)

    Plasmid

    #15079

    Depositor

    Douglas Melton

    Article

    Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

    Insert

    Notch1-IC (Notch1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K1750A

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti_SaCRISPR_GFP

    Plasmid

    #118636

    Purpose

    To clone sgRNA for SaCas9 (SauCas9)

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTP1183

    Plasmid

    #104163

    Purpose

    Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pICherryNeo

    Plasmid

    #52119

    Purpose

    a bicistronic IRES-mCherry containing vector for mammalian expression (CMV promoter)

    Depositor

    Dario Vignali

    Type

    Empty backbone

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5/FRT/TO_Lyn11-HaloTag7

    Plasmid

    #175533

    Purpose

    Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lyn11-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-HA-Sumo1

    Plasmid

    #21154

    Purpose

    Mammalian expression of human Sumo1 with HA tag

    Depositor

    Junying Yuan

    Article

    Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

    Insert

    pcDNA3-HA-Sumo1 (SUMO1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDestTol2pACryGFP

    Plasmid

    #64022

    Purpose

    Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

    Depositor

    Peter Currie, Joachim Berger

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    GFP
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    alpha-Acrystallin promoter

    Available since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCiPS

    Plasmid

    #60011

    Purpose

    Foamy virus helper plasmid expressing Pol, driven by a CMV promoter. Ampicillin resistant.

    Depositor

    David Russell

    Article

    Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

    Insert

    Pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMCL-HA-MAPKK1-11A55B [S218E/S222D]

    Plasmid

    #40809

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S218E, S222D

    Promoter

    CMV

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

27. mTagBFP2-Vimentin-7

    Plasmid

    #55333

    Purpose

    Localization: Intermediate Filaments, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

28. pSG5 Large T

    Plasmid

    #9053

    Depositor

    William Hahn

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FLAG-SKP2

    Plasmid

    #215092

    Purpose

    Expresses FLAG-SKP2 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    SKP2 (SKP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. DsRed-rab7 WT

    Plasmid

    #12661

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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