We narrowed to 15,153 results for: RING;

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Ydf NanoLuc

Plasmid

#225656

Purpose

Reporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.

Depositor

Neha Kamat

Article

Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

Insert

NanoLuciferase

Use

Cell free expression

Tags

Myc

Expression

Bacterial

Promoter

PydfJ115

Available Since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pJL NarX Myc

Plasmid

#225651

Purpose

Expresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.

Depositor

Neha Kamat

Article

Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

Insert

NarX (narX E. coli)

Use

Cell free expression

Tags

Myc

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-80L MBP-SspB Micro

Plasmid

#60410

Purpose

E. coli expression of SspB Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

SspB micro

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

R73Q, Y11K and A15E

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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CMV+ plasmid with onboard mCherry cassette

Plasmid

#169741

Purpose

CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Stargazin-mEGFP-iLID

Plasmid

#178523

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pKD237-3a-2

Plasmid

#90957

Purpose

Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Thiosulfate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23100, Bba_J23105, and PphsA

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2520

Plasmid

#91077

Purpose

Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

OsU6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCast

Plasmid

#200169

Purpose

E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

Depositor

Yong-Su Jin

Article

Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCM189-ParB1::mCherry

Plasmid

#87253

Purpose

Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB1

Tags

mCherry

Expression

Yeast

Promoter

pBAD/tetO

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Talin head-SpyCatcher003

Plasmid

#133566

Purpose

Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

EGFP-Talin head-SpyCatcher003

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ZNF598-TEV-3xFLAG

Plasmid

#105690

Purpose

Expresses C terminally 3xFLAG tagged ZNF598 protein

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Insert

ZNF598 (ZNF598 Human)

Tags

TEV-3xFLAG

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGPA1

Plasmid

#115476

Purpose

Generation of mariner-based transposon mutant libraries in Enterococcus faecium

Depositor

Willem van Schaik

Article

Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pFC333

Plasmid

#87844

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

Depositor

Uffe Mortensen

Article

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
ble (bleomycin resistance marker)

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pYFAC-ubi-Y-pyrG

Plasmid

#184497

Purpose

AMA1 based vector for optimised episomal expression in filamentous fungi, with increased selection stringency using the modified ubi-Y-pyrG marker (high stringency).

Depositor

Yit Heng Chooi

Article

Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus and …

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-NEMOc

Plasmid

#189933

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLAS3W-S100P

Plasmid

#125009

Purpose

express in mammalian cell

Depositor

Ruey-Hwa Chen

Article

Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

Insert

S100P (S100P Human)

Expression

Mammalian

Promoter

CAG

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR5A L3-L2

Plasmid

#158732

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last HD)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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CHMP2A_GFP_N_term

Plasmid

#31805

Depositor

Daniel Gerlich

Article

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

CHMP2A (CHMP2A Human)

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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Equalizer-L plasmid

Plasmid

#169732

Purpose

Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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