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We narrowed to 18,824 results for: nls

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Showing: 761 - 780 of 18824 results

  2. pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid
    #87829
    Purpose
    High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Publication
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    MTS
    NLS
    PTS1
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87829-plasmid-map-sequence-id-175007

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  4. pCS2TAL3-RR

    Plasmid
    #37276
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)
    Depositor
    David Grunwald
    Publication
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Tags
    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    37276-plasmid-map-sequence-id-46873

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  6. pAAV-EF1a-fDIO-Cre (AAV Retrograde)

    Viral prep
    #121675-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Esteban Engel, Alexander Nectow
    Publication
    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30
    Promoter
    EF1a
    Available since
    May 31, 2019
    Availability
    Academic Institutions and Nonprofits only

  9. pmAmetrine-DEVD-tdTomato

    Plasmid
    #18879
    Purpose
    Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato
    Depositor
    Robert Campbell
    Publication
    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mAmetrine-DEVD-tdTomato (CASP3 Human, lab constructed)
    Expression
    Mammalian
    Mutation
    mAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…
    Available since
    Aug. 13, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    18879-plasmid-map-sequence-id-148222

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  11. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid
    #73498
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
    Depositor
    Bruce Conklin
    Publication
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73498-plasmid-map-sequence-id-364403

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  12. pdCas9::BFP-humanized

    Plasmid
    #44247
    Purpose
    Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
    Depositor
    Stanley Qi
    Publication
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 fused to BFP
    Use
    CRISPR and Retroviral
    Tags
    2xNLS & tagBFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    MSCV LTR promoter
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    44247-plasmid-map-sequence-id-60248

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  13. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80063
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Sox10 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80063-plasmid-map-sequence-id-417621

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  14. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Publication
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNASEH1 (RNASEH1 Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Available since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111905-plasmid-map-sequence-id-512037

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  18. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

    Plasmid
    #123615
    Purpose
    CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3(R33A/K34A)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    R33A/K34A in rAPOBEC1, D10A in SpCas9
    Promoter
    CAG
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    123615-plasmid-map-sequence-id-239343

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  20. pEJS578_DD-dSpyCas9-mCherry-APEX2

    Plasmid
    #108570
    Purpose
    Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors
    Depositor
    Erik Sontheimer
    Publication
    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi
    Insert
    dSpyCas9
    Use
    CRISPR and Lentiviral
    Tags
    APEX2, Destabilization domains, Flag, and mCherry…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108570-plasmid-map-sequence-id-328916

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Showing: 761 - 780 of 18824 results