167,642 results

1. pAIP4_6IYE

   Plasmid

   #234237

   Purpose

   Heterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pTripZ-Tet-ON mCherry

   Plasmid

   #187262

   Purpose

   Doxycyclin inducible expression of mCherry

   Depositor

   Alpha Yap

   Article

   Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

   Insert

   mCherry-NLS
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   CMV

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT TO GFP11-LRRK2

   Plasmid

   #231174

   Purpose

   Expresses GFP-11-LRRK2 (wildtype, full length) in mammalian cells

   Depositor

   Samara Reck-Peterson

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   GFP11-LRRK2 (LRRK2 Human)
   

   Tags

   GFP11

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWZL-Blast FAP S624A

   Plasmid

   #207402

   Purpose

   Expresses the S624A mutant of FAP (Fibroblast Activation Protein), which shows no protease activity.

   Depositor

   Jing Yang

   Article

   Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

   Insert

   Fibroblast Activation Protein Alpha (FAP Human)
   

   Use

   Retroviral

   Mutation

   changed Serine 624 to Alanine. It's a protea…

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZL-Blast FAP A657S

   Plasmid

   #207403

   Purpose

   Expresses the A657S mutant of FAP (Fibroblast Activation Protein), which shows partial protease activity.

   Depositor

   Jing Yang

   Article

   Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

   Insert

   Fibroblast Activation Protein Alpha (FAP Human)
   

   Use

   Retroviral

   Mutation

   changed Alanine 657 to Serine. It's an endop…

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

   Plasmid

   #220613

   Purpose

   Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

7. LHZ1494

   Plasmid

   #222104

   Purpose

   Triple sgRNAs expression plasmid in Kluyveromyces marxianus.

   Depositor

   Yongming Wang

   Insert

   sg1RNA, sg4RNA, sg7RNA
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2

   Plasmid

   #218206

   Purpose

   Zebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

   Depositor

   Katarzyna Koltowska

   Article

   Panara et al Development. 2024 May 1;151(9):dev202525

   Insert

   +15.2prox1a
   

   Use

   Zebrafish expression

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LHZ1493

   Plasmid

   #222102

   Purpose

   Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.

   Depositor

   Yongming Wang

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-fDIO-ArgiNLS-mKate2

    Plasmid

    #220607

    Purpose

    Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mKate2
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-fDIO-ArgiNLS-oScarlet

    Plasmid

    #220606

    Purpose

    Flp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    oScarlet
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-ArgiNLS-AausFP1

    Plasmid

    #220595

    Purpose

    Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    AausFP1
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid

    #220614

    Purpose

    Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    

    Use

    AAV and Cre/Lox

    Tags

    3x HA; ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJBL7084

    Plasmid

    #167248

    Purpose

    overexpression of LacI

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pT7-LacI
    

    Expression

    Bacterial

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. LT3GEPIR-WT1shRNA

    Plasmid

    #220560

    Purpose

    To inducibly knockdown WT1 or EWSR1::WT1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

    Insert

    WT1 shRNA (WT1 Human)
    

    Use

    Lentiviral

    Promoter

    TRE3G (TetOP) promoter

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 9AVA

    Plasmid

    #220884

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TREX1 (TREX1 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCW57.1 WT1 +1 ATG

    Plasmid

    #217643

    Purpose

    Dox inducible WT1 cDNA - as transcribed from +1ATG (long isoform)

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    WT1 long isoform (WT1 Human)
    

    Expression

    Mammalian

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR2b-mScarlet

    Plasmid

    #207961

    Purpose

    Gateway entry vector with the mScarlet fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

    Insert

    mScarlet
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_Ef1a_DIO_hTyrNull_HATag

    Plasmid

    #205285

    Purpose

    Truncated version of human Tyrosinase as control virus for Tyrosinase expression

    Depositor

    Matthias Prigge

    Insert

    hTyrosinase (truncated)
    

    Use

    AAV

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pminiTol2-4kb wnt3 promoter-Wnt3EGFP

    Plasmid

    #203793

    Purpose

    Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Wnt3-EGFP for zebrafish transgenesis

    Depositor

    Thorsten Wohland

    Article

    Teh et al Development. 2015 Nov 1;142(21):3721-33.

    Insert

    Wnt3 (wnt3 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mEGFP

    Promoter

    4kb Wnt3 promoter

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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