We narrowed to 16,272 results for: GRN

1. pJMP1221

   Plasmid

   #119260

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti_CRISPRi_sgNeg1

   Plasmid

   #166998

   Purpose

   Lentiviral expression of negative control sgRNA for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   Negative control sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV/3' Box_(GLuc)_INT

   Plasmid

   #68436

   Purpose

   Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three PP7 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSI-359

   Plasmid

   #131131

   Purpose

   gRNA expression vector for A-to-G base editing reporter

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   A-to-G reporter activation gRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP1104

   Plasmid

   #119248

   Purpose

   knockdown pqsC in P. aeruginosa

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA pqsC (P. aeruginosa)
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SL2-sgTelo-MTSa/BFP/pdCas9-C1

   Plasmid

   #162760

   Purpose

   Expressing dCas9 and sgRNA containg MTSa targeting telomeres

   Depositor

   Antony K Chen

   Article

   Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

   Insert

   dCas9 and sgRNA(SL2-sgTelo-MTSa)
   

   Expression

   Mammalian

   Mutation

   dCas9(nuclease deactivated Cas9)

   Promoter

   U6/CMV

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJMP1170

   Plasmid

   #119252

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. GG-EBNA-EEA-guide1-PGK-Puro

   Plasmid

   #102904

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-guide1-PGK-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ133C2.0

   Plasmid

   #99893

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   OsU6

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJZC42

    Plasmid

    #66564

    Purpose

    sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1XPP7
    PCP-VP64 IRES mCherry
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSP2279

    Plasmid

    #70706

    Purpose

    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    D10A and H557A in SaCas9

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ131C2.0

    Plasmid

    #99886

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    OsU6

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ132C2.0

    Plasmid

    #99889

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    OsU6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSI-535

    Plasmid

    #131130

    Purpose

    gRNA expression vector for C-to-T base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    C-to-T reporter activation gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SL2-sgTelo-MTSa/EGFP/pdCas9-C1

    Plasmid

    #162759

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXPR_003 Intergenic control guide 2

    Plasmid

    #193585

    Purpose

    sgRNA control; induces CAS9 cutting in an intergenic region

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgRNA control
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. sg2.0 (PP7) Locus#2

    Plasmid

    #101155

    Purpose

    gRNA with two PCP binding sites

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    Locus #2(tandem repeat)
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMP1217

    Plasmid

    #119258

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJG370

    Plasmid

    #91187

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nAtCas9_D10A+gRNActrl+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJMP1069

    Plasmid

    #119244

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge