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pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

Plasmid

#40571

Depositor

Daniel Gerlich

Article

Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

Insert

Aurora B FRET-sensor N-terminally fused to histone 2B

Use

Tags

CyPet and YPet

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP TEV co-transformation cloning vector (13K-C)

Plasmid

#48317

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 StrepII TEV co-transformation cloning vector (13K-HR)

Plasmid

#48318

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and StrepII

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 Sumo TEV co-transformation cloning vector (13S-S)

Plasmid

#48329

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Sumo

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 StrepII TEV co-transformation cloning vector (13S-HR)

Plasmid

#48326

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and StrepII

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP TEV co-transformation cloning vector (13S-C)

Plasmid

#48325

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 Thioredoxin TEV co-transformation cloning vector (13S-T)

Plasmid

#48330

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Thioredoxin

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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LSM104 CMV-TO-AVPR2-(noExtraV2)-ADAR2(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233390

Purpose

CCR6-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no extra V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR2-ADAR2dd

Use

Tags

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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Coxsackievirus A6 (CVA6) strain JLS10/HeN/CHN/2019 3C protease

Plasmid

#228638

Purpose

Bacterial expression of the Coxsackievirus 63 3C protease

Depositor

Frank von Delft, ASAP Discovery

Insert

Coxsackievirus 63 3C protease

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald

Plasmid

#201187

Purpose

Lentiviral vector plasmid expressing human PTPN2 transcript variant 2 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Bart Nieuwenhuis

Inserts

PTPN2, transcript variant 2 (PTPN2 Human)
Emerald

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLTR-SFFV-PTPN2 transcript variant 3-WPRE-UbC-Emerald

Plasmid

#201188

Purpose

Lentiviral vector plasmid expressing human PTPN2 transcript variant 3 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Bart Nieuwenhuis

Inserts

PTPN2, transcript variant 3 (PTPN2 Human)
Emerald

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET His6 Mocr TEV co-transformation cloning vector (13K-O)

Plasmid

#48320

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Mocr

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 Mocr TEV co-transformation cloning vector (13S-O)

Plasmid

#48327

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Mocr

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

Plasmid

#31482

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (Sce Target) EF Puro

Use

Lentiviral

Tags

Expression

Mutation

mCherry has M9S and M16L mutations. This is what …

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

Plasmid

#128605

Purpose

Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

24xMoonTag
kinesin family member 18b (KIF18B Human)
24xSunTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

Plasmid

#42333

Purpose

This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

Plasmid

#31481

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (Sce target) EF BFP

Use

Lentiviral

Tags

Expression

Mutation

mCherry contains M9S and M16L to reduce backgroun…

Promoter

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42337

Purpose

Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a

Plasmid

#31483

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro

Use

Lentiviral

Tags

Expression

Mutation

Traffic Light 2.0 series have a +3 PEST sequence …

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

Plasmid

#31480

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (ani target)

Use

Lentiviral

Tags

Expression

Mutation

mCherry has M9S, M16L to reduce background mCherr…

Promoter

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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