We narrowed to 28,126 results for: STI

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pYES1L-URA

Plasmid

#84301

Purpose

E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

Depositor

George Church

Article

Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDestattB/CY (pCM327)

Plasmid

#68341

Purpose

Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.

Depositor

Christian Mosimann, Leonard Zon

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Type

Empty backbone

Use

Zebrafish transgenesis

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-mFlagEts1

Plasmid

#86099

Purpose

expresses flag tagged Ets1 in mammalian cells

Depositor

Barbara Graves

Article

Seidel et al Genes Dev. 2002 Jan 1;16(1):127-37.

Insert

Ets1 (Ets1 Mouse)

Tags

Flag tag; kanamycin selection

Expression

Mammalian

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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perbB2-ECFP

Plasmid

#40268

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

erbB2 (ERBB2 Human)

Tags

ecfp

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pSLIK CA Rac1

Plasmid

#84605

Purpose

Lentiviral expression of doxycycline-inducible constitutively active Rac1 and co-expression of Venus

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Constitutively Active, Q61L

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only
pXPR_053

Plasmid

#113591

Purpose

Constitutive expression of a single sgRNA and the fluorophore violet-excited GFP in immune cells

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Commun. 2019 Apr 10;10(1):1668. doi:

Insert

sgRNA cloning site

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMCSF-R-luc

Plasmid

#12420

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP Cep164 (Nigg CW324)

Plasmid

#41149

Depositor

Erich Nigg

Article

Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

Insert

Cep164 (CEP164 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pFastBac HT JS-Munc18c

Plasmid

#135555

Purpose

Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18c

Tags

6xHis tag and TEV site

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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Cdc2-DN-HA

Plasmid

#1889

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdc2 (CDK1 Human)

Tags

HA

Expression

Mammalian

Mutation

D146N, dominant negative

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-FP4-mito

Plasmid

#229692

Purpose

Transient expression of EGFP FP4-mito in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP; Mitochondira FP4 motifs from Listeria ActA

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK7 T194D

Plasmid

#80886

Purpose

mammalian expression of ALK7 T194D

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

ALK7 (Acvr1c Rat)

Tags

HA

Expression

Mammalian

Mutation

T194D (constitutively active)

Promoter

CMV

Available Since

Aug. 25, 2016

Availability

Academic Institutions and Nonprofits only
L4440_BioBrick-hif-1-sgRNA-A

Plasmid

#177784

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV human IRS-1 CAAX

Plasmid

#11238

Depositor

Ronald Kahn

Article

Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

Insert

IRS-1 (IRS1 Human)

Tags

CAAX

Expression

Mammalian

Mutation

C-terminal prenylation motif makes IRS-1 localize…

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pCI-neo.ARRDC1

Plasmid

#38321

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

ARRDC1 (ARRDC1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK13_IDG-K

Plasmid

#135266

Purpose

Gateway destination clone of CDK13 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK13 (CDK13 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSHS207 - Bacterial expression plasmid for WT SpCas9

Plasmid

#101199

Purpose

Bacterial expression plasmid for WT SpCas9

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2017

Availability

Academic Institutions and Nonprofits only

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MMW948: CAG-DmrC-NLS-3xFLAG-VPR

Plasmid

#104565

Purpose

Mammalian expression vector for DmrC fused to VPR activator

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

DmrC-VPR

Tags

3x FLAG and NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK1-WT

Plasmid

#40808

Depositor

Natalie Ahn

Article

Mansour et al Science. 1994 Aug 12;265(5174):966-70.

Insert

MAP2K1 (MAP2K1 Human)

Tags

HA

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pUC19_dTAG-KANSL3-targeting

Plasmid

#170755

Purpose

Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

BSD-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

BSD-P2A-2xHA-dTAG

Mutation

Phenylalanine 36 to valine in FKBP12

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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