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pcDNA5/FRT/TO-3xFLAG-His6-LARP1

Plasmid

#233626

Purpose

Expression of Flag- and His-tagged LARP1

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

LARP1 (LARP1 Human)

Tags

3FLAG-His6

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV BAF170-Flag

Plasmid

#19142

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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CMV500 A-SREBP1

Plasmid

#33357

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Insert

SREBP1 (Srebf1 Cricetulus griseus (hamster))

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
pScPPX2

Plasmid

#112877

Purpose

Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

Depositor

Michael Gray, Ursula Jakob

Article

Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

Insert

PPX1 (PPX1 Budding Yeast)

Tags

6X His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pQE-80L iLID (C530M)

Plasmid

#60408

Purpose

E. coli expression of iLID (C530M)

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

iLID C530M

Tags

His

Expression

Bacterial

Mutation

C530M

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
CAG-mCherry

Plasmid

#108685

Purpose

Strong CAG promoter with mCherry expression for transfection efficacy screening

Depositor

Jordan Green

Article

Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-IRES-Flpo (AAV1)

Viral Prep

#55634-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-EF1a-mCherry-IRES-Flpo (#55634). In addition to the viral particles, you will also receive purified pAAV-EF1a-mCherry-IRES-Flpo plasmid DNA. EF1a-driven mCherry and bicistronic Flp recombinase expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Promoter

EF1a and Ef1a/IRES

Tags

mCherry

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA3.Flag.HERC2-HECT-C4762A.6xHis

Plasmid

#39228

Depositor

Aziz Sancar

Article

Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

Insert

HERC2 HECT Domain (HERC2 Human)

Tags

6xHis and Flag

Expression

Mammalian

Mutation

C4762A

Promoter

T7

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
pORTMAGE-3

Plasmid

#72678

Purpose

Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.

Depositor

Csaba Pál

Article

Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

Insert

mutL E32K

Tags

none

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_SETD8_WT

Plasmid

#82290

Purpose

Gateway Donor vector containing SETD8, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SETD8 (KMT5A Human)

Use

Gateway entry vector

Mutation

P316R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pVSV-G

Plasmid

#138479

Purpose

Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

VSV-G envelop protein

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40

Plasmid

#133560

Purpose

Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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LV TRE-GFP-TMD-DLL1

Plasmid

#194936

Purpose

LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

eGFP-(PDGFR)TMD-rDLL1 ICD (WT)

Use

Lentiviral

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-DDB1 (1140H)

Plasmid

#174849

Purpose

Expression of DDB1

Depositor

Helen Morrison

Insert

DDB1 (DDB1 Human)

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST53 PINK1 N-GFP

Plasmid

#13315

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha-wt-pBabe-puro

Plasmid

#19365

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt human cDNA

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pLeAPS-GFP-blast

Plasmid

#182230

Purpose

Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

GFP-P2A-Blasticidin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2

Plasmid

#14129

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 (TSC2 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRuby2

Plasmid

#40260

Purpose

RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Insert

mRuby2

Tags

His6 and Xpress

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCONR221

Plasmid

#216146

Purpose

Gateway Donor Vector with attP1 and attP2 sites modified from PDONR221 and Ampicillin resistance

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Type

Empty backbone

Use

Cloning backbone

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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