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pDONR221-TAF15 Stop

Plasmid

#84895

Purpose

DONOR vector for Gateway cloning of TAF15

Depositor

Aaron Gitler

Article

Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

TAF15 (TAF15 Human)

Use

Gateway donor

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 Ub-S297

Plasmid

#169965

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Ub

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAPM-D4- miR30-L1221

Plasmid

#115846

Purpose

Control shRNA KD vector

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

control shRNA

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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R777-E221 Hs.RALGDS

Plasmid

#70505

Purpose

Gateway ORF clone of human RALGDS [NM_006266.3] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RALGDS (RALGDS Human)

Use

Gateway entry clone

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-EWSR1 Stop

Plasmid

#84887

Purpose

DONOR vector for Gateway cloning of EWSR1

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5 ∆UBR

Plasmid

#81063

Purpose

Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)

Use

Cloning

Mutation

W1235L (bp g3704t)

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5 ∆HECT

Plasmid

#81065

Purpose

Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)

Use

Cloning

Mutation

C2768A (bp t8302g and g8303c)

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-IL6.Pro

Plasmid

#79491

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Il6 Promoter (IL6 Synthetic, Human)

Use

Multisite gateway entry clone

Promoter

IL6 Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-EF1.Pro

Plasmid

#79488

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

EF1 Promoter (EEF1A1 Synthetic, Human)

Use

Multisite gateway entry clone

Promoter

EF1 Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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p221z-CAS9p-t35s

Plasmid

#118385

Purpose

Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

CAS9p

Use

CRISPR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAsCpf1(TATV)(BB) (pY221)

Plasmid

#89354

Purpose

Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1 RVR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K548V/N552R

Promoter

CBh

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-EWSR1 No Stop

Plasmid

#84888

Purpose

DONOR vector for Gateway cloning of EWSR1 No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Mutation

no stop

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
p221z-dCAS9p-t35s

Plasmid

#118387

Purpose

Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

dCAS9p

Use

CRISPR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-SAR1-no stop

Plasmid

#67345

Purpose

hSar1 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-SARA-no stop

Plasmid

#67347

Purpose

hSara without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
221-AID-RPB1

Plasmid

#195108

Purpose

Targeting vector to introduce AID tag in N-terminal of the human RPB1 gene. Auxin-inducible degron system.

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

AID

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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CDK7 gRNA (BRDN0001162216)

Plasmid

#76077

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK7 (CDK7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Reb_221 aka PAS628

Plasmid

#71233

Purpose

Reb_1 with RebA(G90A)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA(G53A, G90A)

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221 MLK1 E179K

Plasmid

#60541

Purpose

Donor vector for human MLK1 E179K mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway donor vector

Mutation

E179K

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-MICU1-mEF

Plasmid

#31765

Depositor

Vamsi Mootha

Article

Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

Insert

MICU1 (MICU1 Human)

Use

Pdonr gateway

Tags

None

Mutation

Mutated in both EF-hand domains: D231A, E242K, D4…

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR221 MLK1 D294A

Plasmid

#60542

Purpose

Donor vector for human MLK1 D294A mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway donor vector

Mutation

D294A

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
HK2 gRNA (BRDN0001162212)

Plasmid

#76310

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK2 (HK2, HOOK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSEVA221MBPc-AID-T7RNAP

Plasmid

#167975

Purpose

Expresses fusion protein MBPc-AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

MBPc-AID-T7RNAP

Tags

MBPc and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5 ∆PABC

Plasmid

#81064

Purpose

Gateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)

Use

Cloning

Mutation

Y2402A and K2415A (bp t7204g, a7205c, t7206c, a72…

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-POLD3

Plasmid

#183197

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-POLD3

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA221TrxA-AID-T7RNAP

Plasmid

#167976

Purpose

Expresses fusion protein TrxA-AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

TrxA-AID-T7RNAP

Tags

T7RNAP and TrxA

Expression

Bacterial

Promoter

Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-TAF15 No Stop

Plasmid

#84896

Purpose

DONOR vector for Gateway cloning of TAF15 No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

TAF15 (TAF15 Human)

Use

Gateway donor

Mutation

No Stop

Promoter

none

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR221-CCL5.Pro

Plasmid

#79492

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CCL5 Promoter (CCL5 Synthetic, Human)

Use

Multisite gateway entry clone

Promoter

CCL5 Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-rap1-K9R

Plasmid

#84527

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

none

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pDONR221 - myc ULK1 wt

Plasmid

#27623

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available Since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221 Cas9-RFC5

Plasmid

#183200

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-RFC5

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pABM-D4-miR30-L1221

Plasmid

#132933

Purpose

Lentivector for luciferase (control) knockdown and blasticidin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

luciferase shRNA

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 NLRP1 A66V

Plasmid

#166817

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

A66V

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 NLRP1 S1213A

Plasmid

#166824

Purpose

Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

S1213A

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TrpV1 scFv [N221/17]

Plasmid

#182082

Purpose

Mammalian Expression of TrpV1 scFv. Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting TrpV1 (Rattus norvegicus) (Trpv1 Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 NLRP1 P1214R

Plasmid

#166822

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

P1214R

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPU-huTRIMmiR30-L1221

Plasmid

#132937

Purpose

All-in-one huTRIM5 rescue-control shRNA knockdown

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

human TRIM5

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA CrkII Y221F

Plasmid

#50732

Purpose

mammalian expression of CrkII Y221F mutant

Depositor

Kenneth Yamada

Article

Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

Insert

CrkII Y221F (CRK Human)

Expression

Mammalian

Mutation

Y221F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pDONR221 Cas9-HMGB1

Plasmid

#183198

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-HMGB1

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TrpV1 scFv [N221/17]

Plasmid

#190540

Purpose

Mammalian Expression of TrpV1 scFV. Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TrpV1 (Rattus norvegicus) recombinant scFV (Trpv1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Cas9-RFC4

Plasmid

#183199

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-RFC4

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE.puro/TO/Flag/tGFP.HuR.S221D

Plasmid

#110351

Purpose

Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and turboGFP tags

Depositor

Sandra Martha Gomes Dias

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Use

Retroviral

Tags

FLAG and tGFP

Expression

Mammalian

Mutation

S221D, P237S

Promoter

gag

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
XE221 hWnt-16-pCS2P+

Plasmid

#16843

Depositor

Randall Moon

Insert

hWnt-16 (WNT16 Human)

Use

Xenopus expression

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pDONR221-ARFRP1-no stop

Plasmid

#67343

Purpose

hArfrp1 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221 NLRP1 M77T

Plasmid

#166818

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation M77T (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

M77T

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 NLRP1 A54T

Plasmid

#166815

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

A54T

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-hSOX10-Val15AlafsX11

Plasmid

#24732

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Mutation

deleted amino acids 44-62. deletion/frameshift…

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pBS cFgfr2 (CT#221)

Plasmid

#14003

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Fgfr2 (FGFR2 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
PRKCI gRNA (BRDN0001162218)

Plasmid

#76403

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCI

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCI (PRKCI Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-rap1-K651R

Plasmid

#84528

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K651R

Promoter

none

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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