We narrowed to 59,357 results for: cloning

1. C122-E10: mCherry

   Plasmid

   #162892

   Purpose

   Standard Gateway entry clone encoding mCherry fluorescent protein

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   monomeric Cherry fluorescent protein
   

   Use

   Synthetic Biology

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. C121-E06: gLuc

   Plasmid

   #162890

   Purpose

   Standard Gateway entry clone encoding Gaussia luciferase

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Gaussia princeps luciferase
   

   Use

   Synthetic Biology

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. C121-E01: rLuc

   Plasmid

   #162888

   Purpose

   Standard Gateway entry clone encoding Renilla luciferase

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Renilla luciferase
   

   Use

   Synthetic Biology

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. C122-E54: mRuby3

   Plasmid

   #162899

   Purpose

   Standard Gateway entry clone encoding mRuby3 fluorescent protein

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   monomeric Ruby3 fluorescent protein
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFD-NIa-Protease-TVMV

   Plasmid

   #137109

   Purpose

   iFLinkC Linker cloning

   Depositor

   Viktor Stein

   Article

   Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

   Insert

   NIa Protease TVMV
   

   Use

   Synthetic Biology

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57.1

   Plasmid

   #41393

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIX_403

   Plasmid

   #41395

   Purpose

   Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   V5

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. R777-E172 Hs.PIK3R1-nostop

   Plasmid

   #70456

   Purpose

   Gateway ORF clone of human PIK3R1 [NM_181523.2] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   PIK3R1 (PIK3R1 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGW_RUBY_EggCas9

   Plasmid

   #225990

   Purpose

   To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY

   Depositor

   Imran Siddiqi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6-LacZ-BbsI

    Plasmid

    #114671

    Purpose

    For one-step cloning (with blue/ white screening) of an open reading frame of interest for constitutive gene expression

    Depositor

    Lynne Postovit

    Insert

    LacZ
    

    Expression

    Mammalian

    Promoter

    Lac promoter

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. R777-E209 Hs.RAC2

    Plasmid

    #70493

    Purpose

    Gateway ORF clone of human RAC2 [NM_002872.4] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RAC2 (RAC2 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. R777-E239 Hs.RASGRF1

    Plasmid

    #70523

    Purpose

    Gateway ORF clone of human RASGRF1 [NM_001145648.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASGRF1 (RASGRF1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. R777-E151 Hs.PAK3

    Plasmid

    #70435

    Purpose

    Gateway ORF clone of human PAK3 [NM_002578.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PAK3 (PAK3 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. R777-E174 Hs.PIK3R2-nostop

    Plasmid

    #70458

    Purpose

    Gateway ORF clone of human PIK3R2 [NM_005027.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PIK3R2 (PIK3R2 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. R777-E157 Hs.PDGFRB

    Plasmid

    #70441

    Purpose

    Gateway ORF clone of human PDGFRB [NM_002609.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. R777-E158 Hs.PDGFRB-nostop

    Plasmid

    #70442

    Purpose

    Gateway ORF clone of human PDGFRB [NM_002609.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3.3 CAS9 IRES-Dsred

    Plasmid

    #129444

    Purpose

    expression of FnCas9 and DsRed

    Depositor

    Oskar Laur

    Insert

    FnCas9 IRES-Dsred
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. R777-E152 Hs.PAK3-nostop

    Plasmid

    #70436

    Purpose

    Gateway ORF clone of human PAK3 [NM_002578.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PAK3 (PAK3 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS3ndd

    Plasmid

    #97354

    Purpose

    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant

    Depositor

    Jamorn Somana

    Insert

    Nucleoid Disruptive Domain
    

    Use

    Cloning

    Expression

    Bacterial

    Mutation

    changed methionine 1 to isoleucine

    Promoter

    E. coli lacZ promoter

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. R777-E206 Hs.PTEN-nostop

    Plasmid

    #70490

    Purpose

    Gateway ORF clone of human PTEN [NM_000314.5] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PTEN (PTEN Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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