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pLH-sgRNA1-2XboxB

Plasmid

#75391

Purpose

sgRNA1-2XboxB

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XboxB

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAVS1-P-CAG-GFPluc2

Plasmid

#80493

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

GFPluc2

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Synapsin-cyto-mRuby3-iATPSnFR1.0

Plasmid

#102557

Purpose

expresses cytoplasmic ATP sensor in neurons with red reference protein

Depositor

Baljit Khakh

Publication

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

mRuby3-iATPSnFR1.0

Use

AAV

Tags

Ruby3

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AAV-oChIEF-citrine

Plasmid

#50974

Purpose

AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

Depositor

Roger Tsien

Publication

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

ChIEF gene is mammalian codon optimized

Promoter

human synapsin promoter

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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803 pGEX2T PTEN

Plasmid

#10784

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
Opto-mFGFR1_204

Plasmid

#58745

Purpose

Expresses Opto-mFGFR1 (light-activated receptor tyrosine kinase)

Depositor

Harald Janovjak

Publication

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

Opto-mFGFR1

Expression

Mammalian

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-CAG-SPOTlight-U2GCR

Plasmid

#164819

Purpose

Reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Publication

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

SPOTlight-U2GCR

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-oG

Plasmid

#74288

Purpose

To express oG (optimized Glycoprotein) under CAG promoter

Depositor

Edward Callaway

Publication

Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

Insert

oG

Expression

Mammalian

Mutation

chimeric glycoprotein

Promoter

CAG

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Plasmid

#159816

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKM596

Plasmid

#8837

Depositor

David Waugh

Publication

Fox et al FEBS Lett. 2003 Feb 27. 537(1-3):53-7.

Type

Empty backbone

Tags

E. coli MBP

Expression

Bacterial

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA 3.1(-) VSFP2.1

Plasmid

#16255

Depositor

Thomas Knopfel

Publication

Dimitrov et al PLoS ONE. 2007 . 2(5):e440.

Insert

VSFP2.1

Expression

Mammalian

Available since

Jan. 25, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158652

Purpose

making transgenics expressing a UAS driven eGFP and NTR 2.0

Depositor

Jeff Mumm

Publication

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-tTA

Plasmid

#99118

Purpose

An AAV genome that ubiquitously expresses tTA from the CAG promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has service

AAV2

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

CAG

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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BPK2139

Plasmid

#65776

Purpose

Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Mammalian

Promoter

CAG

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-N-RAM-d2tTA-TRE-mKate2

Plasmid

#140275

Purpose

N-RAM, the Npas4-dependent reporter

Depositor

Yingxi Lin

Publication

Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

Has service

AAV1

Insert

mKate2

Use

AAV

Promoter

TRE

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9 KRAB-T2A-GFP

Plasmid

#67620

Purpose

Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 KRAB T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA3.2 TDP-43 5F-L YFP

Plasmid

#84914

Purpose

Mammalian expresion of TDP-43 5F-L YFP

Depositor

Aaron Gitler

Publication

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Mutation

F147L, F149L, F194L, F229L, F231L

Promoter

CMV

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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PGEX3X-GST-PTEN

Plasmid

#20743

Depositor

Alonzo Ross

Publication

Campbell et al J Biol Chem. 2003 Sep 5. 278(36):33617-2

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pL-CRISPR.EFS.PAC

Plasmid

#57828

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-CAG-mRuby2

Plasmid

#99123

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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