Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 11,733 results for: Emb;

Clear keywords
Showing: 2401 - 2450 of 11733 results

  7. pCDH-CB-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73039
    Purpose
    Express Nluc, copGFP dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    chicken beta-actin + CMV enhancer and chicken bet…
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73039-plasmid-map-sequence-id-136043

    Enlarge

  8. TMED2-bio-His

    Plasmid
    #51853
    Purpose
    Expresses full-length Transmembrane emp24 domain-containing protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TMED2 (TMED2 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51853-plasmid-map-sequence-id-79740

    Enlarge

  14. pCAG-FLAG-puro-PTPIP51(1-150_175-470)

    Plasmid
    #170537
    Purpose
    Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection
    Depositor
    Byung Il Lee
    Article
    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:
    Insert
    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    deleted amino acids 151-175
    Promoter
    CAG promoter
    Available Since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only

  15. pCS2+-PIF6-EGFP

    Plasmid
    #154913
    Purpose
    1-100 amino acids of Arabidopsis PIF6 (Gene ID: 825382), tagged with EGFP fluorescent protein. Mammalian codon optimised.
    Depositor
    Clare Buckley, Jonathan Clarke
    Article
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Insert
    phytochrome interacting factor 3-like 2 (PIL2 Mustard Weed)
    Use
    Xenopus/avian/zebrafish
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    1-100 amino acids of Arabidopsis PIF6
    Promoter
    CMV
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154913-plasmid-map-sequence-id-308719

    Enlarge

  17. CD69-His-bio

    Plasmid
    #52328
    Purpose
    Expresses full-length C-type lectin domain family 2 member C ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD69 (CD69 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52328-plasmid-map-sequence-id-81044

    Enlarge

  20. GGE114

    Plasmid
    #120731
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly. It already has some of the parts needed for transformation in Y. lipolytica
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Type
    Empty backbone
    Use
    Cloning/assembly plasmid
    Available Since
    Oct. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    120731-plasmid-map-sequence-id-259124

    Enlarge

  23. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid
    #115775
    Purpose
    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.
    Depositor
    Damian Purcell
    Article
    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37
    Insert
    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    Tags
    EGFP; dsReD
    Expression
    Mammalian
    Mutation
    gp140 uncleaved (1190-91aa, KR > TG; truncated…
    Promoter
    HIV-1 (LTR)
    Available Since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115775-plasmid-map-sequence-id-224438

    Enlarge

  28. pAV123

    Plasmid
    #63189
    Purpose
    pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63189-plasmid-map-sequence-id-331731

    Enlarge

  31. GGE367

    Plasmid
    #120736
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Antibiotic resistance marker for selection after transformation in Y. lipolytica (Marker_hph)
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    Marker_hph
    Use
    Cloning/assembly plasmid
    Available Since
    Oct. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    120736-plasmid-map-sequence-id-259150

    Enlarge

  37. pAV103

    Plasmid
    #63193
    Purpose
    pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Worm and Yeast
    Available Since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    63193-plasmid-map-sequence-id-104872

    Enlarge

  41. pOPS0254

    Plasmid
    #115506
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115506-plasmid-map-sequence-id-224294

    Enlarge

Showing: 2401 - 2450 of 11733 results