We narrowed to 3,530 results for: Braf

1. pCS2-Opto-Acvr1ba-P2A-Acvr2ba

   Plasmid

   #232640

   Purpose

   Optogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expression

   Depositor

   Katherine Rogers

   Insert

   Acvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2+cPLA2-mKate2

   Plasmid

   #162068

   Purpose

   Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Avian, xenopus, zebrafish

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST exorh:mGreenLantern

   Plasmid

   #195988

   Purpose

   Transgenesis backbone

   Depositor

   Christian Mosimann, Nicolas Rohner

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT2-cryR;cldn5bP1Egfp

   Plasmid

   #90158

   Purpose

   contains cldn5b specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   cldn5bP1Egfp (cldn5b Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCK002 pME mCerulean

   Plasmid

   #195956

   Purpose

   Gateway pME fluorophore

   Depositor

   Alexa Burger, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mCerulean
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. E1b-GFP-Tol2-Gateway

   Plasmid

   #37846

   Depositor

   Nadav Ahituv

   Article

   Birnbaum et al Genome Res. 2012 Jun;22(6):1059-68. Epub

   Type

   Empty backbone

   Use

   Zebrafish enhancer assay vector

   Tags

   Expression

   Mutation

   Promoter

   E1b

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-LAMP1-EGFP

   Plasmid

   #169003

   Purpose

   Express rat LAMP1-EGFP in zebrafish

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

   Insert

   rat LAMP1-EGFP
   

   Use

   Unspecified

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. actc1b-Lifeact-mKate2

   Plasmid

   #109493

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to the actin binding lifeact sequence

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-Lifeact-mKate2
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3E-U6a-polg guide

   Plasmid

   #107598

   Purpose

   An entry vector with U6a promoter driving polg guide RNA expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   polg guide RNA sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCK029 exorh:EGFP

    Plasmid

    #195980

    Purpose

    Expression construct

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh:EGFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENO1-dTom-NATr

    Plasmid

    #52208

    Purpose

    Constitutive expression of Candida codon-optimized dTomato with NAT selectable marker

    Depositor

    Robert Wheeler

    Article

    Gratacap et al Dis Model Mech. 2013 Sep;6(5):1260-70. d

    Insert

    dTomato
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for Candida species

    Promoter

    ENO1

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCS2-Mito-mRFP

    Plasmid

    #169001

    Purpose

    Express Mito (Su9)-mRFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Mito (Su9)-mRFP (encoding a mitochondrial matrix targeting sequence of subunit 9 of the V0 ATPase Su9 of N. crassa)
    

    Use

    Unspecified

    Tags

    mRFP

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 469_pME_iCre

    Plasmid

    #171792

    Purpose

    pME-Vector with codon improved iCre sequence. Tol2-kit compatible.

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Insert

    codon-optimized iCre sequence
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DR274-eGFP sgRNA

    Plasmid

    #61051

    Purpose

    for generation of a eGFP specific sgRNA from a T7 promoter

    Depositor

    Filippo Del Bene

    Article

    Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

    Insert

    EGFP sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTol2-nbt-Electra2-P2A-mScarlet

    Plasmid

    #196114

    Purpose

    zebrafish expression of nbt-Electra2-P2A-mScarlet

    Depositor

    Reinhard Köster

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    nbt-Electra2-P2A-mScarlet
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. M-NMn-VP64

    Plasmid

    #48676

    Purpose

    Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null

    Promoter

    CMV

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR5'-attP (pCM254)

    Plasmid

    #68311

    Purpose

    Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Insert

    attP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1457_pDEST_miniTol2_R4-R2_Cryst_mCherry

    Plasmid

    #171796

    Purpose

    pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pME-mTagBFP-CAAX

    Plasmid

    #75149

    Purpose

    Membrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mTagBFP-CAAX
    

    Use

    Middle entry vector for multisite gateway three f…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. actc1b-mKate2-CaaX(tK)

    Plasmid

    #109488

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Kras

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-CaaX(tK)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Tol2-krt4-GFP

    Plasmid

    #97151

    Purpose

    label apical epithelial cells in zebrafish

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    AcGFP
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pT3TS_Transposase

    Plasmid

    #109768

    Purpose

    Codon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Tol2 Transposase
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. M-ST1n-VP64

    Plasmid

    #48675

    Purpose

    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null (D9A D598A H599A N622A)

    Promoter

    CMV

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENO1-iRFP-NATr

    Plasmid

    #129731

    Purpose

    Integration plasmid to overexpress iRFP in Candida albicans

    Depositor

    Robert Wheeler

    Article

    Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

    Insert

    iRFP-Candida albicans codon-optimized
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO1 from Candida albicans

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p3E-mTagBFP

    Plasmid

    #75175

    Purpose

    TagBFP (blue) fluorophore with stop codon for C-terminal fusions

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    TagBFP
    

    Use

    Three prime entry vector for multisite gateway th…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RVD-AA5

    Plasmid

    #173240

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Insert

    NI-NI dimer RVD at Position5
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. RVD-AA9

    Plasmid

    #173244

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Insert

    NI-NI dimer RVD at Position9
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p3E-mTagBFP-CAAX

    Plasmid

    #75176

    Purpose

    Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mTagBFP-CAAX
    

    Use

    Three prime entry vector for multisite gateway th…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. actc1b- mKate2-KDEL-pA

    Plasmid

    #109494

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b- mKate2-KDEL-pA
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV5-Flag-mEPLIN alpha

    Plasmid

    #40955

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

    Insert

    EPLIN alpha (Lima1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pGL3-MTS-ccdb-DddA(G1333C)-UGI-Puro

    Plasmid

    #173228

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGL3-MTS-ccdb-DddA(G1333N)-UGI-Puro

    Plasmid

    #173229

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. actc1b-mKate2-rab27a

    Plasmid

    #109617

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab27a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab27a (rab27a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pME-H2B-mCerulean3

    Plasmid

    #75156

    Purpose

    Nuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    H2B-mCerulean3
    

    Use

    Middle entry vector for multisite gateway three f…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTol2-nbt-Electra1-P2A-mScarlet

    Plasmid

    #196113

    Purpose

    zebrafish expression of nbt-Electra1-P2A-mScarlet

    Depositor

    Reinhard Köster

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    nbt-Electra1-P2A-mScarlet
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p3E-U6a-U6c

    Plasmid

    #107599

    Purpose

    An entry vector with U6a and U6c promoter driving guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Inserts

    U6a promoter
    U6c promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p5E-6xgata2aECEbasp

    Plasmid

    #132968

    Purpose

    gata2a endothelial enhancer (x6) and basal promoter in p5E Gateway vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    gata2a endothelial enhancer (x6) with a b-actin basal promoter
    

    Use

    Gateway vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. M-SPn-VP64

    Plasmid

    #48674

    Purpose

    Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null

    Promoter

    CMV

    Available since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

39. Tol2-ngn1-Trpa1b-2A-mCherry

    Plasmid

    #106425

    Purpose

    For expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Inserts

    cmlc2 promoter
    Trpa1b (trpa1b Zebrafish)
    

    Use

    Gateway

    Tags

    2A-mCherry

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

40. pCMV5-Flag-mEPLIN beta

    Plasmid

    #40956

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

    Insert

    EPLIN beta (Lima1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only