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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,956 results for: VARS

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Showing: 10021 - 10040 of 16956 results

  5. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

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  6. nCaMK2rep-S603A

    Plasmid
    #239625
    Purpose
    Inactive CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.
    Depositor
    F Javier Díez Guerra
    Article
    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029
    Insert
    PSD95.FingR-NES-SynP3-3xmyc (Syn1 Rat, Synthetic)
    Use
    Cre/Lox and Lentiviral
    Tags
    Flag and myc
    Expression
    Mammalian
    Mutation
    Changed serine 603 to Alanine
    Promoter
    hSyn
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239625-plasmid-map-sequence-id-476241

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  10. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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  14. pBA904

    Plasmid
    #122238
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122238-plasmid-map-sequence-id-292011

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Showing: 10021 - 10040 of 16956 results