167,948 results

1. HA-ISceID44A

   Plasmid

   #59424

   Purpose

   Expresses a catalytically inactive form of the endonuclease ISceI

   Depositor

   Tom Misteli

   Article

   Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

   Insert

   endonuclease ISceI mutant (I-SceI )
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   changed aspartate 44 to alanine (D44A)

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. 5HRE/GFP

   Plasmid

   #46926

   Purpose

   hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

   Depositor

   Martin Brown, Thomas Foster

   Article

   Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

   Insert

   5X HRE of VEGF (VEGFA Human)
   

   Tags

   d2EGFP

   Expression

   Mammalian

   Mutation

   five copies of a 35-bp fragment from the hypoxia-…

   Promoter

   EF-1α promoter

   Available Since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. p102_gRNA_Sa_mClover

   Plasmid

   #213778

   Purpose

   Cloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   Sa gRNA
   

   Use

   CRISPR and Lentiviral

   Promoter

   mU6

   Available Since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p103_gRNA_Sp_mClover

   Plasmid

   #213779

   Purpose

   Cloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   Sp gRNA
   

   Use

   CRISPR and Lentiviral

   Promoter

   mU6

   Available Since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5FRT/Flag-WDR5

   Plasmid

   #29759

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   WDR5 (WDR5 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 29, 2013

   Availability

   Academic Institutions and Nonprofits only

6. SPHK2 gRNA (BRDN0001145867)

   Plasmid

   #77094

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCh-Rab5

   Plasmid

   #49201

   Purpose

   expression of Rab5 fused to mCherry

   Depositor

   Gia Voeltz

   Article

   Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

   Insert

   Rab5 (RAB5B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-∆Hapln1

   Plasmid

   #200778

   Purpose

   Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Insert

   Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
   

   Use

   AAV

   Tags

   cfGFP

   Mutation

   deleted lectican binding domain (aminoacids 40-15…

   Promoter

   Synapsin

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC-14-LEXA-QF

   Plasmid

   #46104

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   LexA-QF
   

   Expression

   Insect

   Available Since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. C1(1-29)-TurboID-V5_pLX304

    Plasmid

    #107175

    Purpose

    expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Lentiviral

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid

    #111903

    Purpose

    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.

    Depositor

    Andrew Carter

    Inserts

    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    

    Tags

    8xHis, SNAPf-tag, TEV site, and ZZ-tag

    Expression

    Insect

    Mutation

    Optimised for expression in Sf9 cells.

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PL452-hGH-polyA-PGK-neoR

    Plasmid

    #191681

    Purpose

    Donor plasmid backbone PGK-neoR

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Type

    Empty backbone

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-Merlin S518A

    Plasmid

    #84294

    Purpose

    Expression of Merlin with an alanine mutation at the Pak phosphorylation site

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S518A

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmCherry-T2A-moxMaple3

    Plasmid

    #120875

    Purpose

    Mammalian expression of mCherry and moxMaple3

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

    Inserts

    mCherry
    moxMaple3
    

    Expression

    Mammalian

    Mutation

    from mMaple3, moxMaple3 was engineered by replaci…

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_EGFR_WT

    Plasmid

    #81926

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYeDP60-hATP13A2-WT

    Plasmid

    #171377

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Tags

    BAD tag

    Expression

    Yeast

    Promoter

    URA3

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.3_c-MYC

    Plasmid

    #26818

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-c-MYC-3'UTR (MYC Human)
    

    Expression

    Mammalian

    Mutation

    Sequence begins from second ATG; Synthetic UTR se…

    Available Since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGL003

    Plasmid

    #215335

    Purpose

    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)

    Depositor

    Gene-Wei Li

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    pSpank

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. MGP M155

    Plasmid

    #26527

    Depositor

    David Baltimore

    Article

    O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 1

    Insert

    miR-155 (Mir155 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV2)

    Viral Prep

    #105547-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    EF1a

    Tags

    EGFP

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only