We narrowed to 7,064 results for: tet

1. HA-mRIPK3 K51A-Flag/cHA-pTRIPZ vector

   Plasmid

   #73704

   Purpose

   lentiviral expression of mRIPK3 mutant

   Depositor

   Francis Chan

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   K51A

   Promoter

   CMV/tet

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

2. HA-mRIPK3 D161N-Flag/cHA-pTRIPZ vector

   Plasmid

   #73705

   Purpose

   lentiviral expression of mRIPK3 mutant

   Depositor

   Francis Chan

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   D161N

   Promoter

   CMV/tet

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

3. HA-mRIPK3 D143N-Flag/cHA-pTRIPZ vector

   Plasmid

   #73703

   Purpose

   lentiviral expression of mRIPK3 mutant

   Depositor

   Francis Chan

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   D143N

   Promoter

   CMV/tet

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pTO_CHMP4B_LAP

   Plasmid

   #101850

   Purpose

   Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

   Depositor

   Daniel Gerlich

   Article

   Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

   Insert

   Charged Multivesicular Body Protein 4B (CHMP4B Human)
   

   Use

   Gateway cloning, flp-frt recombination, tet-induc…

   Tags

   Localization and Affinity Purification (LAP) tag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TERT - R865C

   Plasmid

   #213926

   Purpose

   Expresses Mutant TERT R865C

   Depositor

   Coleman Lindsley

   Article

   Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

   Insert

   Mutant TERT R865C (TERT Human)
   

   Use

   Lentiviral

   Tags

   6x His Tag

   Expression

   Mammalian

   Mutation

   Arginine 865 changed to Cysteine

   Promoter

   Tet-responsive promoter PTight, consisting of sev…

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TERT - WT

   Plasmid

   #213827

   Purpose

   Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

   Depositor

   Coleman Lindsley

   Article

   Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

   Insert

   human TERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-responsive promoter PTight, consisting of sev…

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS-TRE-FHDnd1-Ubc-rtTA-I2G

   Plasmid

   #70076

   Purpose

   2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Silent mutations are introduced to escape from RN…

   Promoter

   Tet responsible promoter

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

   Plasmid

   #70075

   Purpose

   2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Y102A. Silent mutations are introduced to escape …

   Promoter

   Tet responsible promoter

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CAG-tTA (AAV2)

   Viral Prep

   #99118-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pAAV-CAG-tTA (#99118). In addition to the viral particles, you will also receive purified pAAV-CAG-tTA plasmid DNA. CAG-driven expresssion of tetracycline-controlled transactivator (tTA). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Promoter

   CAG

   Tags

   None

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

10. pLIX-hN1ICD

    Plasmid

    #91897

    Purpose

    Doxycycline inducible expression of human Notch1 ICD

    Depositor

    Julien Sage

    Insert

    Notch1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codons 1770 to 2555 of human NOTCH1

    Promoter

    TRE promoter, Tet ON

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-mRIPK3 T231A/S232A-Flag/cHA-pTRIPZ vector

    Plasmid

    #73707

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    T231A/S232A

    Promoter

    CMV/tet

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

12. HA-mRIPK3 AAAA-Flag/cHA-pTRIPZ vector

    Plasmid

    #73702

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    448-AAAA-451

    Promoter

    CMV/tet

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pRMC2

    Plasmid

    #68940

    Purpose

    tetracycline-inducible expression vector for Staphylococcus aureus

    Depositor

    Tim Foster

    Article

    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

    Type

    Empty backbone

    Use

    Tet inducible e. coli/staphylococcus shuttle vect…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. iC13 PB-TO-oNgn2-BFP-puro

    Plasmid

    #204733

    Purpose

    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Optimized neurogenin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. iF59 PB-TO-oNgn2-puro

    Plasmid

    #204734

    Purpose

    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Optimized neurogenin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFRT-TODestGFP_RNAseH1

    Plasmid

    #65784

    Purpose

    expresses GFP-tagged RNAseH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Human, Zebrafish)
    

    Use

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFRT-TODestRFP_RNAseH1

    Plasmid

    #65785

    Purpose

    expresses RFP-tagged RNAseH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Human, Zebrafish)
    

    Use

    Tags

    RFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFRT-TODestFLAGHA_RNAseH1

    Plasmid

    #65782

    Purpose

    expresses FLAGHA-tagged RNAsH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Human, Zebrafish)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFRT-DestFLAGHA_RNAseH1

    Plasmid

    #65783

    Purpose

    constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Human, Zebrafish)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-mRIPK3 AAAA/D143N-Flag/cHA-pTRIPZ vector

    Plasmid

    #73706

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    448-AAAA-451 and D143N

    Promoter

    CMV/tet

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pTS35.41

    Plasmid

    #106263

    Purpose

    Polycistronic expression of luc, ekivR using P2A

    Depositor

    Vera Meyer

    Article

    Schuetze et al Microb Cell Fact. 2017 Sep 25;16(1):162.

    Insert

    luc-P2A-ekivR
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Tet-On

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFRT-TODestRFP_HuC

    Plasmid

    #65765

    Purpose

    expresses RFP-tagged HuC in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL3 (ELAVL3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

23. Ubiquiton Yeast Kit

    Plasmid Kit

    #1000000243

    Purpose

    The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.

    Depositor

    Helle Ulrich

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

24. PB-TO-MYOD1-shOct4

    Plasmid

    #182309

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    MYOD1-shOct4 (MYOD1 Human)
    puro-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    shOct4

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBSJL1

    Plasmid

    #71261

    Purpose

    E. coli-Veillonella shuttle plasmid

    Depositor

    Felicia Qi

    Article

    Liu et al Appl Environ Microbiol. 2012 May;78(9):3

    Type

    Empty backbone

    Use

    E. coli-veillonella shuttle plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pFRT-TODestRFP_HuD

    Plasmid

    #65766

    Purpose

    expresses RFP-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V23M mutation in RFP

    Promoter

    CMV, 2x Tet

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

27. AAVS1-SA-2A-NEO-CAG-RTTA3

    Plasmid

    #60431

    Purpose

    neomycin resistance; constitutive promoter driving reverse tet activator

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    rtTA3
    

    Use

    Zfn donor

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFRT-TODestGFP_HuD

    Plasmid

    #65763

    Purpose

    expresses GFP-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFRT-DestFLAGHA_HuB

    Plasmid

    #65758

    Purpose

    constitutively expresses FLAG-HA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pFRT-TODestFLAGHA_HuC

    Plasmid

    #65756

    Purpose

    expresses FLAGHA-tagged HuC in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL3 (ELAVL3 Human)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFRT-TODestGFP_HuB

    Plasmid

    #65761

    Purpose

    expresses GFP-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLVPT-rtTR-KRAB

    Plasmid

    #11777

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, rtTR-KRAB, Tet-off
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFRT-TODestGFP_HuC

    Plasmid

    #65762

    Purpose

    expresses GFP-tagged HuC in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL3 (ELAVL3 Human)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTRE-T2-IRES-His6-Ubiquitin

    Plasmid

    #107107

    Purpose

    Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.

    Depositor

    Michael E. Wright

    Insert

    human ubiquitin UBB (UBB Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Tet

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFRT-TODestFLAGHA_HuD

    Plasmid

    #65757

    Purpose

    expresses FLAGHA-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFRT-DestFLAGHA_HuD

    Plasmid

    #65760

    Purpose

    constitutively expresses FLAG-HA-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pFRT-DestFLAGHA_HuC

    Plasmid

    #65759

    Purpose

    constitutively expresses FLAG-HA-tagged HuC in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL3 (ELAVL3 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pTS37.13

    Plasmid

    #106265

    Purpose

    Polycistronic expression of ekivR, luc, esyn1 using P2A

    Depositor

    Vera Meyer

    Article

    Schuetze et al Microb Cell Fact. 2017 Sep 25;16(1):162.

    Insert

    ekivR-P2A-luc-P2A-esyn1
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Tet-On

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFRT-TODestFLAGHA_HuB

    Plasmid

    #65755

    Purpose

    expresses FLAGHA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCWXPGR-pTF-ERK:MEK

    Plasmid

    #114291

    Purpose

    TET-inducible expression of ERK:MEK fusion protein

    Depositor

    Patrick Salmon

    Insert

    rERK2 and hMEK1 proteins (Mapk1 Rat, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    rat ERK2 fused to human MEK1 with a linker in bet…

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge