Addgene

We narrowed to 4,952 results for: puc

Showing: 2601 - 2650 of 4952 results

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TC512

Plasmid

#164888

Purpose

Pmig-13::Zip(-)::QFAD::NLS::SL2::mCherry

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

zip-QFAD

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-171a-16X

Plasmid

#165452

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Tags

Expression

Mutation

Promoter

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-nonpalindromic-169an-4X

Plasmid

#165413

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Tags

Expression

Mutation

Promoter

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-167a-16X

Plasmid

#164494

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Tags

Expression

Mutation

Promoter

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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Sticky-169a-16X

Plasmid

#164495

Purpose

DNA fragment production for nucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nucleic Acids Res. 2021 Feb 15. pii: 613

Type

Empty backbone

Use

Bacterial vector for dna production

Tags

Expression

Mutation

Promoter

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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TC812

Plasmid

#164894

Purpose

QUAS::CFP, express CFP when co-express with QF

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

CFP

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP1H5

Plasmid

#165065

Purpose

Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFPY2A

Plasmid

#165066

Purpose

Characterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFP

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>Y-FAST-ErB1.2A-yEGFP>T-PGK1-T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0025

Plasmid

#162283

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMV16

Plasmid

#128894

Purpose

Position 6 module- LEU2

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

LEU2

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMV22

Plasmid

#128884

Purpose

Position 3 module- Venus

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Venus

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMV42

Plasmid

#128888

Purpose

Position 4 module- mCherry-STOP

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

mCherry+STOP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMV44

Plasmid

#128889

Purpose

Position 4 module- sfGFP-STOP

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

sfGFP+STOP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV3

Plasmid

#128882

Purpose

Position 3 module- sfGFP

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV20

Plasmid

#128879

Purpose

Position 2 module- Cyc1 promoter

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Cyc1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV19

Plasmid

#128878

Purpose

Position 2 module- Adh1 promoter

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Adh1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV17

Plasmid

#128877

Purpose

Position 2 module- Act1 promoter

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Act1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV2

Plasmid

#128876

Purpose

Position 2 module- Met25 promoter

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Met25 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV14

Plasmid

#128861

Purpose

Position 1 module -2 microns

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

2 microns

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV45

Plasmid

#128885

Purpose

Position 3 module- 3SV40NLS (3x NLS from SV40)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

SV40 NLS

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMV48

Plasmid

#128881

Purpose

Position 2 module- Tdh3 promoter

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Tdh3 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMV1

Plasmid

#128867

Purpose

Position 2 module- Gal1 promoter

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Gal1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMV12

Plasmid

#128893

Purpose

Position 6 module- NatMX

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

NatMX

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMV7

Plasmid

#128891

Purpose

Position 6 module- TRP1

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

TRP1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV6

Plasmid

#128890

Purpose

Position 5 module- Adh1 terminator

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

ADH1 terminator

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV21

Plasmid

#128880

Purpose

Position 2 module- Tef1 promoter

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Tef1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDV

Plasmid

#128860

Purpose

Destination vector for modules assembly

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pE2F3-donor

Plasmid

#113809

Purpose

CRISPR donor plasmid to tag human transcription factor E2F3 with GFP

Depositor

Kevin White

Insert

E2F3 homology arms flanking EGFP-IRES-Neo cassette (E2F3 Human)

Use

CRISPR

Tags

Expression

Mutation

Unknown

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pELF4-donor

Plasmid

#112371

Purpose

CRISPR donor plasmid to tag human transcription factor ELF4 with GFP

Depositor

Kevin White

Insert

ELF4 homology arms flanking EGFP-IRES-Neo cassette (ELF4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pVEZF1-donor

Plasmid

#112341

Purpose

CRISPR donor plasmid to tag human transcription factor VEZF1 with GFP

Depositor

Kevin White

Insert

VEZF1 homology arms flanking EGFP-IRES-Neo cassette (VEZF1 Human)

Use

CRISPR

Tags

Expression

Mutation

rs999907002, hg38:17:57975308:A>T, hg38:17:579…

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAP574-1

Plasmid

#99477

Purpose

3XFlag::Topaz::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::Topaz::Myc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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p249_LTJ_1kbFoxp3wtTemplate

Plasmid

#82664

Purpose

1kb HDR template for murine wildtype Foxp3

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

1kb genomic DNA sequence from murine wildtype Foxp3

Use

Hdr template for gene editing

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p243_LTJ_4kbCD45.1Template

Plasmid

#82663

Purpose

4kb HDR template for murine CD45.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

4kb genomic DNA sequence from murine CD45.1

Use

Hdr template for gene editing

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p250_LTJ_2kbFoxp3wtTemplate

Plasmid

#82665

Purpose

2kb HDR template for murine wildtype Foxp3

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

2kb genomic DNA sequence from murine wildtype Foxp3

Use

Hdr template for gene editing

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP996-1

Plasmid

#99499

Purpose

3XFlag::meGFP::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::meGFP::linker::TEV

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP835-1

Plasmid

#99495

Purpose

TEV::meGFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::meGFP::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP686-1

Plasmid

#99491

Purpose

TEV::eGFP::ER::V5::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::V5::linker::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOG294

Plasmid

#80587

Purpose

dummy module (ccdB) for 2nd generation recipient assembly

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB

Use

Tags

Expression

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGGI000

Plasmid

#48863

Purpose

Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGE003

Plasmid

#48854

Purpose

Provides the At1g04880 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

At1g04880 terminator

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGE002

Plasmid

#48840

Purpose

Provides the WUS terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS terminator

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGD006

Plasmid

#48836

Purpose

Provides the SV40 nuclear localization signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

SV40 NLS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGB006

Plasmid

#48823

Purpose

Provides an ER signal sequence as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

signal sequence ER

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGC014

Plasmid

#48827

Purpose

Provides GFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GFP

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

A206K

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSBC30

Plasmid

#44794

Depositor

Christopher Marx

Article

Chubiz et al PLoS One. 2012;7(10):e47959. doi: 10.137

Insert

FREQ-Seq Barcode: GCTTAG

Use

Tags

Expression

Mutation

Promoter

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSBC32

Plasmid

#44796

Depositor

Christopher Marx

Article

Chubiz et al PLoS One. 2012;7(10):e47959. doi: 10.137

Insert

FREQ-Seq Barcode: TAACTT

Use

Tags

Expression

Mutation

Promoter

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSBC33

Plasmid

#44797

Depositor

Christopher Marx

Article

Chubiz et al PLoS One. 2012;7(10):e47959. doi: 10.137

Insert

FREQ-Seq Barcode: CGGATA

Use

Tags

Expression

Mutation

Promoter

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSBC34

Plasmid

#44798

Depositor

Christopher Marx

Article

Chubiz et al PLoS One. 2012;7(10):e47959. doi: 10.137

Insert

FREQ-Seq Barcode: CAGCAG

Use

Tags

Expression

Mutation

Promoter

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSBC35

Plasmid

#44799

Depositor

Christopher Marx

Article

Chubiz et al PLoS One. 2012;7(10):e47959. doi: 10.137

Insert

FREQ-Seq Barcode: AAGTAG

Use

Tags

Expression

Mutation

Promoter

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSBC36

Plasmid

#44800

Depositor

Christopher Marx

Article

Chubiz et al PLoS One. 2012;7(10):e47959. doi: 10.137

Insert

FREQ-Seq Barcode: GGGACG

Use

Tags

Expression

Mutation

Promoter

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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