176,067 results
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pEGFP-parkin WT
Plasmid#45875DepositorAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUCXMG
Plasmid#204409PurposeExpression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI siteDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromotertacAvailable SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)
Plasmid#196702PurposePlasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.DepositorInserthuman RNaseH1 wild type
ExpressionMammalianAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7pol
Plasmid#59926PurposeExpression vector for T7 RNA polymerase geneDepositorInsertT7 RNA polymerase
ExpressionMammalianPromoterCAGAvailable SinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-ZMYND8
Plasmid#65401PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXPR_502
Plasmid#96923Purposefor CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSFDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcdna3.1 hygro mCherry
Plasmid#128057PurposeMammalian expression vector with N terminal mCherryDepositorTypeEmpty backboneTagsmCherryExpressionMammalianAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-Puro-dTAG (BRD4)
Plasmid#91793PurposePITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertPuro-P2A-2xHA-FKBP_F36V
UseCRISPRExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma9-GFP2
Plasmid#140991PurposeEncodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-C1
Plasmid#60560PurposeExpresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteinsDepositorInsertmTurquoise2
ExpressionMammalianPromoterCMVAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn[himar1C9]
Plasmid#213906PurposeMariner transposon with ATc-inducible himar1C9 transposaseDepositorInsertmariner transposon with cam resistance and ATc-inducible himar1C9 transposase
ExpressionBacterialAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaQ-RLuc8
Plasmid#140982PurposeEncodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaQ-RLuc8 (GNAQ Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-B2M-miniGag-Cas9
Plasmid#228958PurposeminiEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag-Cas9
UseCRISPRExpressionMammalianAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-mScarlet
Plasmid#131000PurposeAAV vector to drive the expression of mScarlet under the control of CamKIIa promoterDepositorHas ServiceAAV8InsertmScarlet
UseAAVPromoterCaMKIIaAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Hygro_GCaMP6s
Plasmid#164591PurposeStable integration and expression of GCaMP6s in mammalian cellsDepositorInsertGCaMP6s
UseLentiviralTags6xHisExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-LC3
Plasmid#110947PurposeExpresses the autophagosome marker LC3 fused to mTurquoise2DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
TagsmTurquoise2ExpressionMammalianAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP13897 - pAAV-AiE0671m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3897)
Plasmid#224038PurposeAiE0671m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neuronsDepositorInsertSYFP2
UseAAVMutationNAPromoterminBGAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-Mito
Plasmid#36208PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorAvailable SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
EB3N-VVDfast-Halo-iLID
Plasmid#190166PurposeExpresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertEB3N-VVDfast-Halo-iLID
Tags-VVDfast-mClover3-iLIDExpressionMammalianMutation1-189 aa from EB3Available SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Tet1
Plasmid#60938PurposeExpresses Tet1 in mammalian cellsDepositorAvailable SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticExpressionMammalianPromoterhUbCAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-ssRFP-GFP-KDEL
Plasmid#128257PurposeFluorescence reporter to monitor ER-phagy activityDepositorInsertssRFPGFPKDEL
UseLentiviralTagsRFPGFPExpressionMammalianPromoterCMVAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMitoLOC
Plasmid#58980PurposeExpression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrilaDepositorInsertsmCherry
GFP
TagspreCOX4 and preSU9ExpressionYeastPromoterADH1 and MET17Available SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS513 EF1alpha-cre
Plasmid#11918PurposeExpresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.DepositorAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV9)
Viral Prep#100845-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiSAMPHv2
Plasmid#167934PurposeAll-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6, EF1aAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2-6xHis
Plasmid#218885PurposeBacterial expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
Tags6xHisExpressionBacterialMutationS99A F102Y F104Y L178SPromoterT7Available SinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-JIP4
Plasmid#164620PurposeExpresses JIP4 in mammalian cells with a GFP tagDepositorAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB506
Plasmid#74848PurposeGateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only
