We narrowed to 32,432 results for: Eng;

1. pcDNA-HisFlag-rUSP19ER-CAmut

   Plasmid

   #155237

   Purpose

   for mammalian overexpression of the mutated ER isoform of rat USP19

   Depositor

   Simon Wing

   Article

   Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

   Insert

   USP19 (ER isoform) (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Cys(545) to Ala

   Promoter

   CMV

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYPQ265H

   Plasmid

   #149554

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   hAID-ziSpyMacCas9(D10A)-UGI
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, R221K, N394K, Protospacer interacting domai…

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQ266B

   Plasmid

   #149555

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   ziSpyMacCas9(D10A)-PmCDA1-UGI
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, R221K, N394K, Protospacer interacting domai…

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQ290-D570A-SRDX

   Plasmid

   #129682

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b-SRDX
   

   Use

   CRISPR; Gateway compatible aaccas12b entry clone

   Expression

   Plant

   Mutation

   D570A; AacCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ291-D573A-SRDX

   Plasmid

   #129683

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   BthCas12b-SRDX
   

   Use

   CRISPR; Gateway compatible bthcas12b entry clone

   Expression

   Plant

   Mutation

   D573A; BthCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBTK110

   Plasmid

   #110576

   Purpose

   BTK Type 2 promoter for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   CP6 Promoter with RBS
   

   Use

   Synthetic Biology

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pBTK112

   Plasmid

   #110577

   Purpose

   BTK Type 2 promoter for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   CP12b Promoter with RBS
   

   Use

   Synthetic Biology

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pBTK113

   Plasmid

   #110578

   Purpose

   BTK Type 2 promoter for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   CP32 Promoter with RBS
   

   Use

   Synthetic Biology

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. p3E-U6a-U6c-sod2 guide

   Plasmid

   #107604

   Purpose

   An entry vector with U6a and U6c promoter driving sod2 guide RNAs expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   sod2 guide RNA sequences
   

   Use

   CRISPR

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p3E-U6a-U6c-sod1 guide

    Plasmid

    #107603

    Purpose

    An entry vector with U6a and U6c promoter driving sod1 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    sod1 guide RNA sequences
    

    Use

    CRISPR

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p3E-U6a-U6c-uqcrc1 guide

    Plasmid

    #107602

    Purpose

    An entry vector with U6a and U6c promoter driving uqcrc1 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    uqcrc1 guide RNA sequences
    

    Use

    CRISPR

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMV-TRP1

    Plasmid

    #65335

    Purpose

    standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TRP1
    

    Use

    Synthetic Biology

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Gal4pBS3-Pleum

    Plasmid

    #60330

    Purpose

    Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Expression

    Yeast

    Available Since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. Gal4pBS1-Pleum

    Plasmid

    #59993

    Purpose

    Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Expression

    Yeast

    Available Since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pMXs-B5

    Plasmid

    #31709

    Depositor

    Duanqing Pei

    Article

    Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

    Insert

    miR-363 (Mir363 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pNTPTP alpha (S204A)

    Plasmid

    #17699

    Depositor

    Xinmin Zheng

    Article

    Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

    Insert

    PTPalpha S204A (PTPRA Human)
    

    Expression

    Mammalian

    Mutation

    S204A

    Available Since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pKH3-TRIM28

    Plasmid

    #45569

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAAV-hsyn-GRAB_DA2m

    Plasmid

    #140553

    Purpose

    Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    The 2nd generation DA sensor GRAB_DA2m (medium affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 6xHsa.NFKB:d2mRFP1

    Plasmid

    #239991

    Purpose

    Destabilized red fluorescent protein (d2mRFP1) under transcriptional control of NF-kB activation

    Depositor

    Raquel Espin-Palazon

    Inserts

    mRFP1
    PEST
    

    Promoter

    cfos minimal promoter

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDIRECT_22C

    Plasmid

    #91135

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV5-Flag-HDAC3

    Plasmid

    #63676

    Purpose

    Mammalian expression of Flag tagged HDAC3

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

    Insert

    HDAC3 (Hdac3 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pAAV-TRE-DIO-FLPo

    Plasmid

    #118027

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-DIO-FLPo
    

    Use

    AAV

    Promoter

    TRE

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid

    #122650

    Purpose

    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    PylRS-AF, Pyl-tRNACUA
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDIRECT_23C

    Plasmid

    #91140

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVTHM-mSox2-3xflag

    Plasmid

    #206376

    Purpose

    Expresses 3xflag fused mouse SOX2 in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223249

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pC3.1-caggs-Crimson-CAAX

    Plasmid

    #190041

    Purpose

    Red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

    Depositor

    Michael Lin

    Article

    Ning et al Front Cell Dev Biol. 2022 Jun 29;10:8934

    Insert

    Crimson
    

    Tags

    CAAX

    Expression

    Mammalian

    Promoter

    caggs

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAVS1-Puro CAG J591-scFV T-CAR

    Plasmid

    #171961

    Purpose

    Donor plasmid for J591 T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Harris et al J Immunol Regen Med. 2023 May;20:100074.

    Insert

    Synthetic J591-scFV CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1(+)- e18

    Plasmid

    #131670

    Purpose

    Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEGFP-TRIM28

    Plasmid

    #45568

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    G34S mutation in EGFP

    Promoter

    CMV

    Available Since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pAAV-hsyn-GRAB_rDA1h

    Plasmid

    #140557

    Purpose

    Expresses the red fluorescent high-affinity DA sensor GRAB_rDA1h in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    Red fluorescent DA sensor GRAB_rDA1h (high affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-hsyn-GRAB_DA2h

    Plasmid

    #140554

    Purpose

    Expresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    The 2nd generation DA sensor GRAB_DA2h (high affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGEX-5X-TRIM28

    Plasmid

    #45570

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Ptac

    Available Since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. AAVS1-Puro CAG PSMA nanobody T-CAR

    Plasmid

    #171962

    Purpose

    Donor plasmid for PSMA nanobody T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Harris et al J Immunol Regen Med. 2023 May;20:100074.

    Insert

    Synthetic anti-PSMA nanobody CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-hsyn-GRAB_rDA1m

    Plasmid

    #140556

    Purpose

    Expresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    Red fluorescent DA sensor GRAB_rDA1m (medium affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 6xHsa.NFKB:d2EGFP

    Plasmid

    #239990

    Purpose

    Destabilized green fluorescent protein (d2EGFP) under transcriptional control of NF-kB activity

    Depositor

    Raquel Espin-Palazon

    Insert

    Destabilized EGFP
    

    Promoter

    cfos minimal promoter

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBBR_Cas9_sp1

    Plasmid

    #154851

    Purpose

    Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

    Depositor

    John van der Oost

    Article

    Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

    Inserts

    Spcas9 gene
    sgRNA scaffold
    

    Use

    CRISPR

    Tags

    6xHis

    Mutation

    Codon harmonized variant for expression in Rhodob…

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Oct4-IRES-eGFP-PGK-Neo

    Plasmid

    #48681

    Purpose

    For knock-in donor vector by CRISPR/CAS system

    Depositor

    Rudolf Jaenisch

    Article

    Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    CRISPR

    Tags

    GFP

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDIRECT_25H

    Plasmid

    #91145

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pDIRECT_21C

    Plasmid

    #91130

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Ad-Flag-XBP1s

    Plasmid

    #63678

    Purpose

    Adenoviral vector for XBP1s expression

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    spliced

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Sc++

    Plasmid

    #155011

    Purpose

    Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

    Insert

    Sc++
    

    Expression

    Mammalian

    Mutation

    T1227K, Loop Substitution from S. anginosus

    Promoter

    EF1ɑ

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAAV-TRE-dDIO-vCre

    Plasmid

    #118029

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-dDIO-vCre
    

    Use

    AAV

    Mutation

    See depositor comments below

    Promoter

    TRE

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pQE-BR1

    Plasmid

    #160333

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

    Insert

    BR1
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGL3-MTS-G3635A-L17-Q2L7-T2176N-UGI-Puro

    Plasmid

    #209647

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-Q2L7-T2176N
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pRSET-pr-Kaede (Kaede-A69T)

    Plasmid

    #99228

    Purpose

    Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-Kaede
    

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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