We narrowed to 14,071 results for: cas9

1. pTK Cas9-2A-Citrine

   Plasmid

   #92393

   Purpose

   Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   Cas9 2A Citrine
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   thymidine kinase promoter

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AW01_pU.CAG.eSpCas9(1.1).rBGpA

   Plasmid

   #199255

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1) nuclease

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

   Insert

   High specificity eSpCas9(1.1) nuclease
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   K848A K1003A R1060A

   Promoter

   CAG promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-EF1a-Cas9GFP-W

   Plasmid

   #200100

   Purpose

   Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GFP
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xFLAG-dCas9/p-bacteria

   Plasmid

   #64325

   Purpose

   Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR; Tetracycline-inducible

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pLtetO-1

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX165-LZ3 Cas9

   Plasmid

   #140561

   Purpose

   Expresses LZ3 Cas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Mutation

   N690C T769I G915M N980K

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIVT-dCas9-mCherry

   Plasmid

   #174443

   Purpose

   in vitro transcription template for dCas9-mCherry

   Depositor

   Michael Lampson

   Article

   Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

   Insert

   Cas9 Endonuclease Dead
   

   Use

   In vitro transcription template

   Tags

   mCherry

   Promoter

   T7 SP6

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-His6-FnCas9GFP

   Plasmid

   #130966

   Purpose

   expression of FnCas9-GFP in bacterial cells

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   HA-NLS-FnCas9
   

   Use

   CRISPR

   Tags

   6xHis-mEGFP-TEV-HA-NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Cas9-NRCH

   Plasmid

   #136926

   Purpose

   Expresses Cas9-NRCH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9-NRCH
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. M3D395A-M14-dCas9

   Plasmid

   #134780

   Purpose

   Expression of fusion protein M3D395A-M14-dCas9 in Mammalian cells

   Depositor

   Shu-Bing Qian

   Article

   Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

   Insert

   METTL3/METTL14 methyltransferase domain and inactive dCas9
   

   Expression

   Mammalian

   Mutation

   D395A

   Promoter

   CMV

   Available Since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xFLAG-dCas9/pMSCVhyg

    Plasmid

    #134323

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIVT-dCas9-EGFP

    Plasmid

    #174442

    Purpose

    in vitro transcription template for dCas9-EGFP

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Cas9 Endonuclease Dead
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Promoter

    T7 SP6

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUF1-dCas9-GCN5

    Plasmid

    #122509

    Purpose

    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

    Depositor

    Lubin Jiang

    Article

    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

    Insert

    dCas9-GCN5
    

    Use

    CRISPR

    Tags

    3*FLAG

    Mutation

    dCas9(D10A,H840A)

    Promoter

    PfHsp86

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC93-pmax-dCas9VP160

    Plasmid

    #48225

    Purpose

    dCas9VP160 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCas9-P2A-BFP

    Plasmid

    #149447

    Purpose

    Expresses Cas9 and BFP in a lentiviral vector.

    Depositor

    Jonathan Chernoff

    Article

    Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

    Insert

    P2A-BFP
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpCas9-CBE6a-V106W

    Plasmid

    #215824

    Purpose

    Express CBE6a-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_eA3A T31A-NG-nCas9

    Plasmid

    #163539

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDNR-SpCas9-Gem

    Plasmid

    #80424

    Purpose

    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Gem
    

    Use

    CRISPR

    Tags

    Geminin

    Mutation

    codon optimized for human

    Promoter

    T7

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAW90.dCas9-YY1

    Plasmid

    #104373

    Purpose

    Expresses dCas9-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    dCas9-YY1
    

    Available Since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.minBG-CI-SaCas9-W3SynpA

    Plasmid

    #231369

    Purpose

    minBG-driven SaCas9, with short synthetic terminator sequence.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    SaCas9
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. NLS-dCas9-trCIB1

    Plasmid

    #64119

    Purpose

    Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    dCas9-trCIB1 fusion
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    dCas9 (D10A H840A, catalytically inactive), trCIB…

    Promoter

    CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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