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Plasmid#48081PurposeLuciferase Reporter with Gal4 DNA binding site upstream of hACTB promoterDepositorInsertLuciferase
UseTagsExpressionMammalianMutationremoved hsp70 promoter, inserted amplified 615 bp…PromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HRW-CRY2-VHH(GFP)
Plasmid#126024Purposeexpress RFP-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
UseTagsRFPExpressionInsectMutationWTPromoterHsp70Available sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
HS-DN-Plk1
Plasmid#105949Purposeheat shock inducible transgenesisDepositorInsertPlk1 (PLK1 Human)
UseTagsExpressionBacterialMutationDominant negative K82 RPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HFW-CRY2-VHH(GFP)
Plasmid#126027Purposeexpress Flag-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
UseTagsFlagExpressionInsectMutationWTPromoterHsp70Available sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE3-VP64-P10
Plasmid#104607PurposeExpresses TALE3 under UAS controlDepositorInsertTALE3
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)
Plasmid#126026Purposeexpress RFP-tagged LARIAT variant for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHRolig-VHH(GFP)
UseTagsRFPExpressionInsectMutationCRY2 E490GPromoterHsp70Available sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)
Plasmid#126025Purposeexpress RFP- and mCer-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)
UseTagsRFPExpressionInsectMutationWTPromoterHsp70Available sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE1-VP64-P10
Plasmid#104606PurposeExpresses TALE1 under UAS controlDepositorInsertTALE1
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE4-VP64-P10
Plasmid#104608PurposeExpresses TALE4 under UAS controlDepositorInsertTALE4
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK011
Plasmid#69875Purposeexpression of Channelrhodopsin-2 T159C mutantDepositorInsertChannelrhodopsin-2
UseUnspecifiedTagsExpressionMutationT159CPromoterDrosophila Hsp70Available sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-2
Plasmid#175362PurposeG418R backbone for recombinant protein overexpression with Drosophila ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK010
Plasmid#69874Purposeexpression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutantDepositorInsertChannelrhodopsin-2
UseUnspecifiedTagsExpressionMutationD156C (XXL)PromoterDrosophila Hsp70Available sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11274:pHR-hU6-crTet-HSP-hyperdCas12a-miniVPR-CMV-mCherry
Plasmid#210603Purposeheat shock promoter - activated hyperdCas12a-based transcriptional activatorDepositorInserthyperdCas12a-miniVPR
UseCRISPR and LentiviralTagsExpressionMutationPromotertruncated hsp70Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1
Plasmid#175360PurposeZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS3-Syn21-V5-mCherry-P10
Plasmid#104617PurposeExpresses V5 tagged mCherry upon binding of TALE3 to VAS3DepositorInsertSyn21-V5-mCherry-P10-MhcSA
UseTagsV5ExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro
Plasmid#210605Purposeheat shock promoter - activated hyperdCas12a-based transcriptional base editorDepositorInsertABE8e-hyperdCas12a
UseCRISPR and LentiviralTagsExpressionMutationPromotertruncated hsp70Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST-GFP-RpL10Ab
Plasmid#71729PurposeExpression of Drosophila melanogaster Rpl10Ab-GFP fusionDepositorInsertRpL10Ab (RpL10Ab Fly)
UseTagsEGFPExpressionInsectMutationPromoterhsp70Available sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
HS-OS-Plk4-mKate2
Plasmid#105944Purposeheat shock inducible transgenesis, non phoshorylable version of Plk4DepositorInsertPlk4 (Plk4 Mouse)
UseTagsmKate2ExpressionBacterialMutation1002 to 3704 of Plk4/ deletion of S282-S305.PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
HS-aPKC-CAAX-GFP
Plasmid#105946Purposeheat shock inducible transgenesisDepositorInsertaPKC (Prkcz Rat)
UseTagsCAAX and GFPExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HDAC4 WT
Plasmid#52977Purposeinsect expression of HDAC4 WTDepositorInsertHDAC4 (HDAC4 Fly)
UseTagsFLAGExpressionInsectMutation*PromoterHsp70Available sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAS-hopscotch
Plasmid#37301DepositorInserthopscotch (hop Fly)
UseTagsExpressionInsectMutationPromoterHsp70Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
D32_Ld_354780
Plasmid#135848PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertHsp70 protein, putative
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10
Plasmid#104616PurposeExpresses HA tagged Citrine upon binding of TALE1 to VAS1DepositorInsertSyn21-Citrine-HA-P10-MhcSD
UseTagsHAExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
UAS-Xbp1spliced
Plasmid#80628Purposeexpression of Xbp1 RB isoform in DrosophilaDepositorInsertXbp1 (Xbp1 Fly)
UseTagsExpressionInsectMutationRB isoformPromoterhsp70Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar(T->G)-GFP
Plasmid#63695Purpose3.2 kb marine armor plate enhancer with a single T->G bp change driving GFP reporterDepositorInsert3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
UseTagsEGFPExpressionMammalianMutationT->G bp changePromoterhsp70Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCaShs-hopscotch
Plasmid#37299DepositorInserthopscotch (hop Fly)
UseTagsExpressionInsectMutationPromoterHsp70Available sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HSPH2
Plasmid#19454DepositorInsertheat shock 70kDa protein 4 (HSPA4 Human)
UseTagsExpressionMammalianMutationmutations A381T, T732A, and an insertion of "…PromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
HS-EB3-GFP
Plasmid#105948Purposeheat shock inducible transgenesisDepositorInsertEB3 (MAPRE3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 T196E
Plasmid#52975Purposeinsect expression of SIK3 T196E constitutively active mutantDepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationT196E constitutively active mutantPromoterHsp70Available sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 WT
Plasmid#52972Purposeinsect expression of SIK3 WTDepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationPromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 K70M
Plasmid#52973Purposeinsect expression of SIK3 K70M (kinase dead mutant)DepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationK70M kinase dead mutantPromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ths pUAST-HA
Plasmid#69769PurposeThisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertThisbe (FGF8like-1)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationEncodes amino acids 1-739 of Thisbe (NP_610701.2)…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HDAC4 3A
Plasmid#52978Purposeinsect expression of HDAC4 3A constitutive active mutantDepositorInsertHDAC4 (HDAC4 Fly)
UseTagsFLAGExpressionInsectMutationS239,573,748A constitutively active*PromoterHsp70Available sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAS-CFP-MEK
Plasmid#42077DepositorInsertDrosophila MEK ortholog (Dsor1 Fly)
UseTagsCFPExpressionInsectMutationPromoterHsp70Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASt-FMW-attB-AthHen1
Plasmid#104958PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila using somatic tissue Gal4 driversDepositorInsertHen1 (HEN1 Mustard Weed)
UseTagsFLAG MycExpressionInsectMutationPromoterHsp70 promoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
hs-FL-Kek1-myc2
Plasmid#41170DepositorInsertKek1 (kek1 Fly)
UseTags2X mycExpressionInsectMutationPromoterHsp70Available sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJC-repo1.9kb-TALE4-VP64-P10
Plasmid#104612PurposeExpresses TALE4 under the control of a 1.9kb repo enhancer elementDepositorInsert1.9kb repo enhancer element (repo Fly)
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-GFP
Plasmid#175363PurposeZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cellsDepositorInsertsgGFP
UseTagsExpressionInsectMutationF64L, S65C, and I167TPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-PA
Plasmid#175446PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-Protein A and BiP Secretion PeptideExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 T196A
Plasmid#52974Purposeinsect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)DepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationT196A LKB1 phosphorylation defective mutantPromoterHsp70Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A
Plasmid#69792PurposePebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble - Drosophila guanine nucleotide exchange factor
UseP element-based puast vector for gal4-regulated e…TagsHA tagExpressionInsectMutationEncodes Pbl (NP_729306.1) lacking last 9 amino ac…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
HS-DN-Cep152-mKate2
Plasmid#105943Purposeheat shock inducible transgenesisDepositorInsertCep152 (CEP152 Human)
UseTagsmKate2ExpressionBacterialMutationdominant negative (AA 1-512)PromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC-ase0.8kb-TALE3-VP64-P10
Plasmid#104610PurposeExpresses TALE3 under the control of a 0.8kb ase enhancer elementDepositorInsert0.8kb ase enhancer element (ase Fly)
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASt-SIK3 S563A
Plasmid#52976Purposeinsect expression of SIK3 S563A PKA phosphorylation defective mutantDepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationS563A PKA phosphorylation defectivePromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
fru-T2A-QF2 HDR plasmid
Plasmid#141099PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru geneDepositorInsertfru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
UseDonor templateTagsExpressionMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2h70CRET(#325)
Plasmid#184060Purposecyclofen-inducible CRE activation in zebrafish transient transgenicDepositorInsertCRE-ERT2
UseZebrafish transient transgenesisTagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-Hsp70-4Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-CR-PA
Plasmid#175445PurposeHiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsBiP Secretion Peptide and Thrombin-Protein AExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A DH domain
Plasmid#69794PurposePebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble DH domain
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationContains only the Pebble DH domainPromoterhsp70Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-PA-10H-SIII
Plasmid#175443PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-Protein A-10His-TwinStrep and BiP Secretion Pe…ExpressionInsectMutationPromoterFused Actin-HSP70Available sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-10H-SIII
Plasmid#175444PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-10His-TwinStrep and BiP Secretion PeptideExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only