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pUCmini-iCAP-PHP.eB

Plasmid

#103005

Purpose

non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

Synthetic construct isolate AAV-PHP.eB VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

132 Articles

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human beta-catenin pcDNA3

Plasmid

#16828

Purpose

Mammalian expression of human beta-catenin with Flag tag

Depositor

Eric Fearon

Publication

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

60 Articles

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Flag-SIRT1

Plasmid

#1791

Purpose

Mammalian expression of flag-tagged SIRT1

Depositor

Michael Greenberg

Publication

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Tags

Flag

Expression

Mammalian

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

47 Articles

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E-cadherin-GFP

Plasmid

#28009

Depositor

Jennifer Stow

Publication

Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

Insert

E-cadherin (CDH1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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3X ERE TATA luc

Plasmid

#11354

Purpose

Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

Depositor

Donald McDonnell

Publication

Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

Insert

ERE

Use

Luciferase

Expression

Bacterial

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

64 Articles

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pcDNA3.1-p300

Plasmid

#23252

Depositor

Warner Greene

Publication

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

p300 (EP300 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

45 Articles

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mRFP-Rab5

Plasmid

#14437

Purpose

Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

Depositor

Ari Helenius

Publication

Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

Insert

Rab 5 (RAB5A Human)

Tags

mRFP

Expression

Mammalian

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

82 Articles

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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

Plasmid

#71236

Purpose

Express sgRNA and dCas9-KRAB from lentiviral vector

Depositor

Charles Gersbach

Publication

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A Puro
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

140 Articles

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Lenti-iCas9-neo

Plasmid

#85400

Purpose

Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

Depositor

Qin Yan

Publication

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Insert

Flag-iCas9-P2A-GFP

Use

Lentiviral

Tags

Flag-iCas9-P2A-GFP

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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pEGFP-LC3 (human)

Plasmid

#24920

Purpose

Mammalian expression of LC3 fused to EGFP

Depositor

Toren Finkel

Publication

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

LC3 (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

104 Articles

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pHAGE TRE dCas9-KRAB

Plasmid

#50917

Purpose

Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

TRE

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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pgRNA-humanized

Plasmid

#44248

Purpose

Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

Depositor

Stanley Qi

Publication

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

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HER2 WT

Plasmid

#16257

Depositor

Mien-Chie Hung

Publication

Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

Insert

HER2 (ERBB2 Human)

Expression

Mammalian

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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Luciferase-pcDNA3

Plasmid

#18964

Depositor

William Kaelin

Publication

Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

Insert

Firefly Luciferase

Expression

Mammalian

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

44 Articles

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GFP-RelA

Plasmid

#23255

Depositor

Warner Greene

Publication

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Insert

p65 (RELA Human)

Tags

EGFP

Expression

Mammalian

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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PX458-SPNS1-sg1

Plasmid

#198566

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Publication

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg1 (SPNS1 Human)

Use

CRISPR

Expression

Mammalian

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg2

Plasmid

#198567

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Publication

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg2 (SPNS1 Human)

Use

CRISPR

Expression

Mammalian

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Flag-His-ATM wt

Plasmid

#31985

Purpose

Mammalian expression of human ATM with Flag and His tags

Depositor

Michael Kastan

Publication

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM wild-type (ATM Human)

Tags

Flag and HIS

Expression

Mammalian

Mutation

3' UTR of 400 nucl.

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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pdCas9-bacteria

Plasmid

#44249

Purpose

aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

Depositor

Stanley Qi

Publication

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dCas9 (bacteria)

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

pLtetO-1

Available since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

82 Articles

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piRFP670-N1

Plasmid

#45457

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Has service

Cloning Grade DNA

Insert

iRFP670

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

58 Articles

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