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Showing: 3001 - 3030 of 3030 results

  1. TFORF1273

    Plasmid
    #142774
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DPF2 (DPF2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142774-plasmid-map-sequence-id-395843

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  3. pSB63 - pL0_fLUC-I (CDS1)

    Plasmid
    #123180
    Purpose
    Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
    Use
    Luciferase; Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites
    Promoter
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123180-plasmid-map-sequence-id-241283

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  5. pTRIP TRE-BI GFP10-RHOA/RBD-GFP11

    Plasmid
    #182232
    Purpose
    Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain
    Depositor
    Stéphanie Cabantous
    Article
    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.
    Inserts
    ras homolog family member A (RHOA Human)
    rhotekin RHO binding domain (RBD)
    Use
    Lentiviral
    Tags
    GFP10 (split-GFP tripartite) and GFP11 (M4)
    Expression
    Mutation
    Promoter
    TRE bidirectional
    Available since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    182232-plasmid-map-sequence-id-359581

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  6. mCherry-CRY2-INPP5E

    Plasmid
    #79561
    Purpose
    expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Use
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. mCherry-CRY2-INPP5E(D556A)

    Plasmid
    #79563
    Purpose
    expression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5E
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Use
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(I)

    Plasmid
    #204700
    Purpose
    This plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.
    Depositor
    Michael Rosen
    Article
    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1
    Insert
    BRD4-p300(I) (BRD4, EP300 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    deletion of all TF-bidning domains and HAT in p300
    Promoter
    CMV
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204700-plasmid-map-sequence-id-405170

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  11. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)

    Plasmid
    #204699
    Purpose
    This plasmid allows for inducible expression of engineered BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells.
    Depositor
    Michael Rosen
    Article
    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1
    Insert
    BRD4-p300 (BRD4, EP300 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    deletion of all TF-bidning domains in p300
    Promoter
    CMV
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204699-plasmid-map-sequence-id-405091

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  13. Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid
    #135766
    Purpose
    This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.
    Depositor
    Nadia Mercader Huber
    Article
    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6
    Insert
    Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    UAS
    Available since
    Jan. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    135766-plasmid-map-sequence-id-270202

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  15. pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i1-14-GFP10

    Plasmid
    #180348
    Purpose
    mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180348-plasmid-map-sequence-id-363921

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  16. pTRIP TRE BI_SEPT9_i1-14-GFP11_SEPT9_i1-14-GFP10

    Plasmid
    #180351
    Purpose
    mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT9_i1 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN9_v1 (SEPTIN9 Human)
    SEPTIN9_v1 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180351-plasmid-map-sequence-id-357912

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  18. pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i3-14-GFP10

    Plasmid
    #180349
    Purpose
    mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180349-plasmid-map-sequence-id-357927

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  19. pTRIP TRE BI_SEPT9_i3-14-GFP11_SEPT9_i3-14-GFP10

    Plasmid
    #180352
    Purpose
    mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT9_i3 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN9_v3 (SEPTIN9 Human)
    SEPTIN9_v3 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180352-plasmid-map-sequence-id-357936

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  20. pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEPT7 Gmut2

    Plasmid
    #180353
    Purpose
    mammalian co-expression of human SEPT7 Gmut2 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN7 (SEPTIN7 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 H279D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180353-plasmid-map-sequence-id-357939

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  21. pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 Gmut2

    Plasmid
    #180359
    Purpose
    mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 H279D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180359-plasmid-map-sequence-id-358019

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  22. pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2

    Plasmid
    #180361
    Purpose
    mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 H279D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180361-plasmid-map-sequence-id-358006

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  24. pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10

    Plasmid
    #180357
    Purpose
    mammalian co-expression of human SEPT2 NCmut fused to GFP10 and of human SEPT2 NCmut fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN2 (SEPTIN2 Human)
    SEPTIN 2 (SEPTIN2 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT2 F20D, V27D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180357-plasmid-map-sequence-id-358018

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  25. pTRIP TRE BI_SEPT9_i1 NCmut-14-GFP11_SEPT9_i1 NCmut-14-GFP10

    Plasmid
    #180358
    Purpose
    mammalian co-expression of human SEPT9_i1 NCmut fused to GFP10 and of human SEPT9_i1 NCmut fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN9_v1 (SEPTIN9 Human)
    SEPTIN9_v1 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT9_i1 I281D, M288D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180358-plasmid-map-sequence-id-358016

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  26. pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_GFP10-14-SEPT7 Gmut1

    Plasmid
    #180354
    Purpose
    mammalian co-expression of human SEPT7 Gmut1 fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN7 (SEPTIN7 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 W269A, H279D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180354-plasmid-map-sequence-id-357925

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  27. pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i1 Gmut-14-GFP10

    Plasmid
    #180355
    Purpose
    mammalian co-expression of human SEPT9_i1 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 )
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 W269A, H279D and SEPT9_i1 W520A, H530D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180355-plasmid-map-sequence-id-357948

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  28. pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i3 Gmut-14-GFP10

    Plasmid
    #180356
    Purpose
    mammalian co-expression of human SEPT9_i3 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 )
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 W269A, H279D and SEPT9_i3 W502A, H512D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180356-plasmid-map-sequence-id-358007

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  29. pTRIP TRE BI_SEPT9_i3 NCmut-14-GFP11_SEPT9_i3 NCmut-14-GFP10

    Plasmid
    #180360
    Purpose
    mammalian co-expression of human SEPT9_i3 NCmut fused to GFP10 and of human SEPT9_i3 NCmut fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN9_v3 (SEPTIN9 Human)
    SEPTIN9_v3 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT9_i3 I263D, M270D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180360-plasmid-map-sequence-id-357944

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  30. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid
    #213031
    Purpose
    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration
    Depositor
    Donna Romero, Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    Use
    Luciferase
    Tags
    Firefly luciferase antisense intron (3' UTR)
    Expression
    Mammalian
    Mutation
    Promoter
    pTRE bidirectional tet-on
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213031-plasmid-map-sequence-id-422025

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Showing: 3001 - 3030 of 3030 results