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We narrowed to 15,312 results for: ef1

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

Plasmid

#162817

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF6

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF6(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

Plasmid

#162818

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF7b

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF7b(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

Plasmid

#162819

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF10

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF10(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

Plasmid

#162820

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF9c/14

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF9c/14(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

Plasmid

#162821

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b_alt

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF3b_alt(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*SMIM38

Plasmid

#162808

Purpose

STELLA plasmid for amber suppression of N-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)SMIM38

Use

Piggybac

Tags

Expression

Mammalian

Mutation

SMIM38 (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4x7SKPylT_EF1_Ub-*PIGBOS

Plasmid

#162809

Purpose

STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)PIGBOS

Use

Piggybac

Tags

Expression

Mammalian

Mutation

PIGBOS (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_EEF1A2_GTP-EFTU-D2

Plasmid

#109919

Purpose

Protein expression and purification of EEF1A2_GTP-EFTU-D2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EEF1A2_GTP-EFTU-D2 (EEF1A2, EEF1A1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L5-EF1_-R3

Plasmid

#141037

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

EF1_ promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-GpNLuc

Plasmid

#135949

Purpose

Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

GpNLuc LumiFluor

Use

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-OgNLuc

Plasmid

#135951

Purpose

Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

OgNLuc LumiFluor

Use

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Ad-EEF1D-S133E

Plasmid

#131577

Purpose

Expresses EEF1D with S133E mutation in mammalian cells

Depositor

Haiyan Xu

Insert

Eukaryotic translation elongation factor 1 delta (EEF1D Human)

Use

Adenoviral

Tags

No

Expression

Mutation

changed Serine 133 to Glutamic acid

Promoter

CMV

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pIAGO-AceF1

Plasmid

#114461

Purpose

Expression of late polyene biosynthetic genes in streptomycetes

Depositor

Patrick Caffrey

Article

Stephens et al Biosci Biotechnol Biochem. 2013;77(4):88

Insert

AceDI + AceDII + AceN + AceM

Use

Tags

Expression

Bacterial

Mutation

Promoter

ermE

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC Glu39Ala_P2A_Hygro_Barcode

Plasmid

#120512

Purpose

Barcoded lentiviral vector to express MYC Glu39Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC Glu39Ala (MYC Human)

Use

Lentiviral

Tags

Expression

Mutation

Point mutation changing Glutamic Acid to Alanine …

Promoter

EF1a

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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YCe2741 HC_Kan_EF1ap_p18

Plasmid

#100698

Purpose

part designed to occupy position 18 of EMMA. Functional category: Constitutional promoter

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

EF1a promoter (EEF1A1 Human)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2Y641F

Plasmid

#22635

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Aug 31. ():.

Insert

Ror2 Y641F (Ror2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 641 to Phenylalanine

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pEF1a-mRor2Y645F

Plasmid

#22636

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Aug 31. ():.

Insert

Ror2 Y645F (Ror2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 645 to Phenylalanine

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pEF1a-mRor2Y646F

Plasmid

#22637

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Aug 31. ():.

Insert

Ror2 Y646F (Ror2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 646 to Phenylalanine

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pT3-EF1A-MYC-IRES-luc

Plasmid

#129775

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

Depositor

Amaia Lujambio

Article

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Insert

human MYC (MYC Human)

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-IRES-Dre

Plasmid

#55633

Purpose

Expresses Dre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Inserts

mCherry
Dre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a and Ef1a/IRES

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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