167,948 results

1. AAV_NLS-SaCas9-NLS-VPR

   Plasmid

   #68496

   Purpose

   AAV vector containing SaCas9 fused to VPR

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   SaCas9-VPR
   

   Use

   AAV and CRISPR

   Tags

   VPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-UBC-FLEX-XRI-FLAG

   Plasmid

   #178057

   Purpose

   Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   XRI-FLAG
   

   Use

   AAV

   Tags

   FLAG-MBP

   Expression

   Mammalian

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. VV073: 1xCox8 - GCaMP5G in fubi

   Plasmid

   #58509

   Purpose

   Expresses the calcium sensor GCaMP5G in the mitochondria

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   1xCox8 - GCaMP5G
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   a-CamKII

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT7-7 asyn A30P

   Plasmid

   #105726

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

   Insert

   alpha-synuclein A30P (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   A30P

   Promoter

   T7

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p5sheLL

   Plasmid

   #46953

   Purpose

   encode HPV5 L1 and L2, Bicistronic, too large to self-package

   Depositor

   John Schiller

   Article

   Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

   Insert

   HPV5L1+L2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR-GFP-WT1 +KTS N terminal HA

   Plasmid

   #217639

   Purpose

   Entry vector with WT1 +KTS mutant with N terminal HA for gateway cloning

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 +KTS isoform (WT1 Human)
   

   Use

   Gateway cloning

   Tags

   HA

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENTR-GFP-WT1 -KTS N terminal HA

   Plasmid

   #217641

   Purpose

   Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 -KTS isoform (WT1 Human)
   

   Use

   Gateway cloning

   Tags

   HA

   Mutation

   p.408_410delKTS

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. XLone-BSD SPI1 P2A mCherry

   Plasmid

   #140031

   Purpose

   Tunable and temporal expression control of SPI1 and mCherry

   Depositor

   Xiaoping Bao

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGGS-4591NES

   Plasmid

   #138377

   Purpose

   Booster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   Booster-ROCK
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBABE-neomycin-PC-MYC

    Plasmid

    #184550

    Purpose

    Retroviral vector to express human PC with MYC tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 28A_APEX2_MSP1D1

    Plasmid

    #170923

    Purpose

    Express APEX2_MSP1D1 in Bl21

    Depositor

    Huan Bao

    Article

    Bao et al Commun Biol. 2021 Apr 30;4(1):514. doi:

    Insert

    APEX2-MSP
    

    Expression

    Bacterial

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSG5-EGFP

    Plasmid

    #49246

    Purpose

    encodes enhanced green fluorescent protein (EGFP)

    Depositor

    Rebecca Riggins

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

13. HPRT

    Plasmid

    #22884

    Depositor

    James Thomson

    Article

    Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

    Insert

    hypoxanthine phosphoribosyltransferase-1 (HPRT1 Human)
    

    Use

    Cre/Lox; Homologous recombination

    Mutation

    PGK, NeoR, and BGH polyA are between the two geno…

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac HT JS-Rab10wt

    Plasmid

    #135557

    Purpose

    Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Rab10 (Rab10 Mouse)
    

    Tags

    6xHis tag, TEV site

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7-7 asyn G51D

    Plasmid

    #105747

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

    Insert

    alpha-synuclein G51D (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    G51D

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIBA2-Int-SpyTag

    Plasmid

    #198038

    Purpose

    Expression plasmid for producing SpyTagged stalled secretion variant of intimin

    Depositor

    Jack Leo

    Article

    Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

    Insert

    eaeA
    

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    lacks native signal peptide (amino acids 1-39)

    Promoter

    tet

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SCP1-CMVenh-luc

    Plasmid

    #22216

    Depositor

    James Kadonaga

    Article

    Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

    Insert

    SCP1 (super core promoter 1)-CMVenh luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFD4d-U6-1:white1-U6-3:white1

    Plasmid

    #84005

    Purpose

    pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter

    Depositor

    Phillip Zamore

    Article

    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

    Inserts

    white sgRNA-1
    white sgRNA-1
    

    Use

    CRISPR

    Promoter

    U6:1, U6:3, and dU6-1; dU6-3

    Available Since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYZ183

    Plasmid

    #98425

    Purpose

    Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYZ184

    Plasmid

    #98426

    Purpose

    Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pOPINE-3C-mCherry

    Plasmid

    #214060

    Purpose

    Expression of fluorescent fusion proteins

    Depositor

    Ray Owens

    Insert

    mCherry
    

    Tags

    Hexahistidine

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCI-syn-fRCaMP2

    Plasmid

    #125246

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMP2
    

    Expression

    Mammalian

    Mutation

    D396A

    Promoter

    synapsin

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pD434-SR mouse Alpha-synuclein

    Plasmid

    #89073

    Purpose

    bacterial expression of mouse Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pVITRO-HPV8 L1L2

    Plasmid

    #52598

    Purpose

    Expresses HPV8 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV8 L1
    HPV8 L2
    

    Expression

    Mammalian

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-4T1 mouse PARG (439-959)

    Plasmid

    #132615

    Purpose

    expresses mouse PARG(439-959) in E. coli.

    Depositor

    Wenqing Xu

    Article

    Wang et al PLoS One. 2014 Jan 21;9(1):e86010. doi:

    Insert

    PARG (439-959)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p663-UBC-ER-TurboID-V5_IDG-K

    Plasmid

    #214453

    Purpose

    Gateway destination clone of ER-TurboID (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ER-TurboID-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HC87 pEYFPN1 anti-Homer1 nanobody

    Plasmid

    #135223

    Purpose

    Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody HC87 (Homer1 L. glama (llama))
    

    Tags

    6xHis-HA-EYFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL3-noSV40-humanCOL2A1enhancer

    Plasmid

    #173961

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Enhancer region from COL2A1 gene
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSEVA-gRic6T

    Plasmid

    #106401

    Purpose

    E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

    Depositor

    Sheng Yang

    Article

    Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    pj23119

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p006kanGFP

    Plasmid

    #58534

    Purpose

    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Expression

    Bacterial

    Promoter

    T5 between two lac operators

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AICSDP-55:DMD-mEGFP

    Plasmid

    #159744

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Insert

    DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR223_SPP1_WT_V5

    Plasmid

    #82981

    Purpose

    Gateway Donor vector containing SPP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    SPP1 (SPP1 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBS mouse IRS-2

    Plasmid

    #11372

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Expression

    Bacterial

    Available Since

    March 1, 2006

    Availability

    Academic Institutions and Nonprofits only

35. pCLX-pTF-R1-DEST-R2-EBR65

    Plasmid

    #45952

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAC-dCLK

    Plasmid

    #33153

    Depositor

    Michael Rosbash

    Article

    McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17.

    Insert

    CLK (Clk Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Promoter

    Actin 5C

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pVITRO1-BFP-CAAX

    Plasmid

    #184459

    Purpose

    Plasma membrane-targeted EBFP2 expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    EBFP2-CAAX
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNB23

    Plasmid

    #103058

    Purpose

    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGp

    Depositor

    Dirk Bumann

    Article

    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

    Insert

    GFP-OVA
    

    Expression

    Bacterial

    Promoter

    hydrogen peroxide-inducible promoter katGp

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLX301-KANK1

    Plasmid

    #121985

    Purpose

    overexpress of KANK1

    Depositor

    GuangJun Zhang

    Article

    Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

    Insert

    KANK1 (KANK1 Human)
    

    Use

    Lentiviral

    Promoter

    CMV promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKB01_mOrange2

    Plasmid

    #69102

    Purpose

    Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mOrange2
    

    Use

    Synthetic Biology

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge