165,637 results

1. pHR_TRE_mcherry-P2A-Pcad

   Plasmid

   #133807

   Purpose

   inducible expression of mCherry and Pcad in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   cadherin 3 (CDH3)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSFS2A-CaKan

   Plasmid

   #189565

   Purpose

   Codon optomized Kan resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans using G418 selection

   Depositor

   Richard Bennett

   Article

   Park et al mSphere. 2022 Aug 15:e0034722. doi: 10.1

   Insert

   CaKan
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYZ038

   Plasmid

   #200227

   Purpose

   universal template to build tRNA-gRNA array for multiplex editing CREEPY

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   tRNA-gRNA scaffold
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZH81 pET21b Rat1-His

   Plasmid

   #231650

   Purpose

   Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   Rat1 (RAT1 Budding Yeast)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZH82 pET28a-Rai1

   Plasmid

   #231651

   Purpose

   Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   rai1 (RAI1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZH23 Dual G-less containing ySSA1 polyA

   Plasmid

   #231647

   Purpose

   yeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassette

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   SSA1 polyA termination region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZH20 Dual G-less containing yCYC1 polyA

   Plasmid

   #231646

   Purpose

   yeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassette

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   CYC1 polyA termination region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. hCathepsin L

   Plasmid

   #11250

   Purpose

   Mammalian expression of human cathepsin L

   Depositor

   Hyeryun Choe

   Article

   Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

   Has Service

   Cloning Grade DNA

   Insert

   cathepsin L (CTSL Human)
   

   Use

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. myc-KIF5C

   Plasmid

   #127618

   Purpose

   N-terminal myc-tagged mouse KIF5C

   Depositor

   Josef Kittler

   Article

   Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

   Insert

   KIF5C (Kif5c Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. YFP NLS Beta-Actin G13R

    Plasmid

    #60615

    Purpose

    Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    G13R

    Promoter

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pF1-Hs Cdk1/cyclin B1/Cks1

    Plasmid

    #177329

    Purpose

    Co-expression of the human active Cdk1/cyclin B1/Cks1 kinase complex

    Depositor

    Andreas Boland

    Article

    Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

    Inserts

    Cyclin-dependent kinase 1 (CDK1 Human)
    G2/mitotic-specific cyclin-B1 (CCNB1 Human)
    Cyclin-dependent kinases regulatory subunit 1 (CKS1B Human)
    

    Use

    Tags

    2 x Strep-tag and 8 x His

    Expression

    Insect

    Mutation

    Promoter

    p10 and polh

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF0799

    Plasmid

    #141705

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ATF3 (ATF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-Ef1a-InteinC-CreC

    Plasmid

    #187615

    Purpose

    AAV vector encoding C-terminal portion of split-Intein-mediated split-Cre

    Depositor

    Il Hwan Kim

    Article

    Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

    Insert

    InteinC-CreC
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSD141B

    Plasmid

    #218139

    Purpose

    Lentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD56-eGFP-IRES2-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-Rab11-DN (S25N)

    Plasmid

    #101046

    Purpose

    Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    RAB11 (RAB11A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S25N

    Promoter

    CMV

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBP-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199202

    Purpose

    Gateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expression

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    split-intein-Gal4[21-881]-C-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI-neo Flag HAUSP

    Plasmid

    #16655

    Depositor

    Bert Vogelstein

    Article

    Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

    Insert

    HAUSP (USP7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFlp-Km

    Plasmid

    #222334

    Purpose

    Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cl, flp, and sacB

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDZ2FE_Pogz_1-1410

    Plasmid

    #105674

    Purpose

    Expresses Flag-tagged full-length human POGZ protein

    Depositor

    Thanos Halazonetis

    Insert

    POGZ (POGZ Human)
    

    Use

    Tags

    2X Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.CMV.Luc.IRES.EGFP.SV40

    Plasmid

    #105533

    Purpose

    AAV expression of Luciferase and EGFP from CMV promoter

    Depositor

    James M. Wilson

    Insert

    Luciferase
    

    Use

    AAV and Luciferase

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. Tet-O-FUW-ZEB2-P2A-mCherry

    Plasmid

    #203857

    Purpose

    Tet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    ZEB2 (ZEB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-Ef1a-CreN-InteinN

    Plasmid

    #187614

    Purpose

    AAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre

    Depositor

    Il Hwan Kim

    Article

    Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

    Insert

    CreN-InteinN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSIQ

    Plasmid

    #216238

    Purpose

    All-in One IQ transgene switch, empty vector

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ABE7.10-F148A

    Plasmid

    #132946

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    ABE7.10(F148A)
    

    Use

    CRISPR; Base editor

    Tags

    Expression

    Mammalian

    Mutation

    ABE7.10(F148A)

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRDA_336

    Plasmid

    #179095

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (Cas9-NG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCas-CmR(+)

    Plasmid

    #113633

    Purpose

    Chloramphenicol resistant derivative of pCas plasmid

    Depositor

    Olivier Lichtarge

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    Chloramphenicol resistance gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Pcat

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TFORF0144

    Plasmid

    #141536

    Purpose

    Lentiviral vector for overexpressing the PHOX2A transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PHOX2A (PHOX2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPB-cT3G-cERP2-FOXL2

    Plasmid

    #192914

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of FOXL2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOXL2 (FOXL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEVOL-pAzF[TAG]

    Plasmid

    #164579

    Purpose

    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    araBAD, glnS, and proK

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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