Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

176,036 results

Showing: 181 - 210 of 176036 results

  1. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV1)

    Viral Prep
    #44361-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available Since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only

  5. pAAV-Ef1a-fDIO EYFP (AAV9)

    Viral Prep
    #55641-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Tags
    EYFP (Flp-dependent)
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only

  6. CD63-pEGFP C2

    Plasmid
    #62964
    Purpose
    full length human CD63 cloned into pEGFP C2
    Depositor
    Paul Luzio
    Insert
    CD63 molecule (CD63 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CD63 has 1 silent mutation compared to the coding…
    Promoter
    CMV
    Available Since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62964-plasmid-map-sequence-id-207850

    Enlarge

  7. pALOD4

    Plasmid
    #111026
    Purpose
    Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A
    Depositor
    Arun Radhakrishnan
    Article
    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d
    Insert
    Domain 4 of Anthrolysin O
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Mutations: S404C, C472A
    Promoter
    T7
    Available Since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111026-plasmid-map-sequence-id-214044

    Enlarge

  12. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

    Enlarge

  15. AAV pCAG-FLEX-EGFP-WPRE (AAV2)

    Viral Prep
    #51502-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do
    Promoter
    CAG
    Tags
    EGFP (Cre-dependent)
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. pcDNA3-Cyto-ExRai-CKAR2

    Plasmid
    #236097
    Purpose
    Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.
    Depositor
    Jin Zhang
    Article
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Insert
    ExRai-CKAR2-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236097-plasmid-map-sequence-id-473746

    Enlarge

  17. pcDNA3-PM-ExRai-CKAR2

    Plasmid
    #236098
    Purpose
    Plasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.
    Depositor
    Jin Zhang
    Article
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Insert
    Lyn-ExRai-CKAR2
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236098-plasmid-map-sequence-id-473738

    Enlarge

  18. pAAV-EF1a-fDIO-Cre (AAV Retrograde)

    Viral Prep
    #121675-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Esteban Engel, Alexander Nectow
    Article
    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30
    Promoter
    EF1a
    Available Since
    May 31, 2019
    Availability
    Academic Institutions and Nonprofits only

  19. AAV-CamKIIa-jGCaMP8s-WPRE (AAV9)

    Viral Prep
    #176752-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-CamKIIa-jGCaMP8s-WPRE (#176752). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8s-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8s. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CamKIIalpha
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only

  22. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Steven Carr, Samuel Myers
    Article
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    97421-plasmid-map-sequence-id-466895

    Enlarge

  23. pLVX-TetOne-bleo-atpD-K155Q

    Plasmid
    #248068
    Purpose
    Lentiviral expression of a catalytically inactive mutant of the beta subunit of ATPGobble. Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-puro-atpG (#248067) for a LOF control.
    Depositor
    Denis Titov
    Article
    Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.
    Insert
    atpD
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    Lysine 155 to Glutamine, codon-optimized for expr…
    Promoter
    TRE3GS
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    248068-plasmid-map-sequence-id-493802

    Enlarge

  24. pLVX-TetOne-puro-atpG

    Plasmid
    #248067
    Purpose
    Lentiviral expression of gamma subunit of ATPGobble. Includes ampR and puroR (puromycin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-bleo-atpD (#248066) for active ATPGobble.
    Depositor
    Denis Titov
    Article
    Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.
    Insert
    atpG (atpG E. coli)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    codon-optimized for expression in human cells
    Promoter
    TRE3GS
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    248067-plasmid-map-sequence-id-493799

    Enlarge

  25. pLVX-TetOne-bleo-atpD

    Plasmid
    #248066
    Purpose
    Lentiviral expression of beta subunit of ATPGobble. Includes ampR and bleoR (zeocin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-puro-atpG (#248067) for active ATPGobble.
    Depositor
    Denis Titov
    Article
    Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.
    Insert
    atpD
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    codon-optimized for expression in human cells
    Promoter
    TRE3GS
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    248066-plasmid-map-sequence-id-493795

    Enlarge

  26. pLVX-TetOne-bsd-atpA

    Plasmid
    #248065
    Purpose
    Lentiviral expression of alpha subunit of ATPGobble. Includes ampR and bsdR (blasticidin). Use together with pLVX-TetOne-bsd-atpD (#248066) and pLVX-TetOne-atpG (#248067) for active ATPGobble.
    Depositor
    Denis Titov
    Article
    Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.
    Insert
    atpA
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in human cells
    Promoter
    TRE3GS
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    248065-plasmid-map-sequence-id-493789

    Enlarge

  28. pcDNA3-ER-ExRai-CKAR2

    Plasmid
    #236099
    Purpose
    Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to ER surface.
    Depositor
    Jin Zhang
    Article
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Insert
    CYP450-ExRai-CKAR2
    Tags
    N-terminal 27 amino acids from CYP450
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236099-plasmid-map-sequence-id-473790

    Enlarge

Showing: 181 - 210 of 176036 results