We narrowed to 4,775 results for: SRS

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pGS-21a his6-GST-WTPhi29DNAP

Plasmid

#166863

Purpose

To produce a dual affinity-tagged Phi29 DNA polymerase

Depositor

Paul Blainey, Stephen Quake

Article

Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

Insert

Phi29 Polymerase codon-optimized for overexpression in E. coli

Tags

GST and his6

Expression

Bacterial

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET28c-F30-Broccoli

Plasmid

#66788

Purpose

Expresses Broccoli in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Article

Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

Insert

Broccoli in the F30 scaffold

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA

Plasmid

#46759

Purpose

gRNA cloning vector for in vitro transcription of target gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

gRNA core

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 V5 hHR23B

Plasmid

#13054

Depositor

Steven Grossman

Article

Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

Insert

hHR23B (RAD23B Human)

Tags

His and V5

Expression

Mammalian

Available Since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pBAD-LacI

Plasmid

#46394

Purpose

Overproduction of LacI

Depositor

Florian Hollfelder

Article

Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

Insert

LacI

Tags

6xHIS tag

Expression

Bacterial

Mutation

silent T to C mutation at position 19

Promoter

arabinose promoter

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGTetRnls

Plasmid

#26599

Depositor

Peter Andrews

Article

Zafarana et al Stem Cells. 2009 Apr . 27(4):776-82.

Insert

Tetracycline repressor (TetR E.Coli)

Tags

nls

Expression

Mammalian

Available Since

Nov. 15, 2010

Availability

Academic Institutions and Nonprofits only

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PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113611

Purpose

PPI reporter

Depositor

Xiaokun Shu

Article

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

mu opioid receptor

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113610

Purpose

PPI reporter

Depositor

Xiaokun Shu

Article

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

beta 2 adrenergic receptor

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-BroccoliSamSensor#1

Plasmid

#124363

Purpose

Overexpression of circular RNA (Broccoli-based SAM sensor)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-BroccoliSamSensor#1

Expression

Mammalian

Promoter

U6+27

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLIGFP_W220F

Plasmid

#46772

Purpose

Protein overexpression with the modified LacIWF repressor

Depositor

Florian Hollfelder

Article

Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

Insert

LacI_W220F (lacI )

Mutation

Tryptophan 220 replaced by Phenylalanine

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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PCDNA3-PAR2-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

Plasmid

#113609

Purpose

PPI reporter

Depositor

Xiaokun Shu

Article

Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

Insert

PAR2

Expression

Mammalian

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Cygnet2.1

Plasmid

#19737

Depositor

Wolfgang Dostmann

Article

Honda et al Proc Natl Acad Sci U S A. 2001 Feb 27. 9

Insert

Cygnet 2.1

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pT7goldRNA

Plasmid

#47930

Purpose

In vitro transcription of golden gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

golden gRNA (slc24a5 Zebrafish)

Use

CRISPR

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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Hy_pMT EGFP SV40 pA

Plasmid

#69911

Purpose

Expresses EGFP in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

EGFP

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAM3110

Plasmid

#40247

Depositor

Susan Golden

Article

Ditty et al Microbiology. 2005 Aug;151(Pt 8):2605-13

Insert

attP Site

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pVP64

Plasmid

#22287

Depositor

Louise Prakash

Article

Pages et al Genes Dev. 2009 Jun 15. 23(12):1438-49.

Insert

REV1 (REV1 Budding Yeast)

Tags

4xHA

Expression

Yeast

Available Since

April 22, 2010

Availability

Academic Institutions and Nonprofits only
pAM2314

Plasmid

#40246

Depositor

Susan Golden

Article

Ditty et al Microbiology. 2005 Aug;151(Pt 8):2605-13

Insert

Polylinker

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pT7mitfagRNA

Plasmid

#47931

Purpose

in vitro transcription of mitfa gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

mitfa gRNA (mitfa Zebrafish)

Use

CRISPR

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pNXRVd-HACre

Plasmid

#78404

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
ura4+ (ura4 Fission Yeast)

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pT7ddx19gRNA

Plasmid

#47932

Purpose

in vitro transcription of ddx19 gRNA

Depositor

Wenbiao Chen, Susan Wente

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

ddx19 gRNA (ddx19 Zebrafish)

Use

CRISPR

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzpHS2

Plasmid

#172971

Purpose

pWSK29 with S. flexneri wzzpHS2 gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzPHS2 gene from Shigella flexneri

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzO139

Plasmid

#172970

Purpose

pWSK29 with Vibrio cholerae wzzO139 gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzz gene from Vibrio cholerae O139

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRNA2

Plasmid

#174005

Purpose

Used to generate stable mRNA for heterologous expression experiments (no polyA)

Depositor

Stephen Ikeda

Article

Williams et al Front Neurosci. 2010 Nov 4;4:181. doi: 1

Insert

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzBSF

Plasmid

#172967

Purpose

pWSK29 with S. flexneri 2a wzzBSF gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzB gene from Shigella flexneri

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pNXRVg-HACre

Plasmid

#78405

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
arg3+ (arg3 Fission Yeast)

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNXRVh-HACre

Plasmid

#78406

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
leu1+ (leu1 Fission Yeast)

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNXRVk-HACre

Plasmid

#78408

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
ade6+ (ade6 Fission Yeast)

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPS1356

Plasmid

#1379

Depositor

Pamela Silver

Article

Shen et al Genes Dev 1998 Mar 1;12(5):679-91.

Insert

NPL3 (NPL3 Budding Yeast)

Tags

Myc

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pAM1619

Plasmid

#40244

Depositor

Susan Golden

Article

Mackey et al Methods Mol Biol. 2007;362:115-29.

Insert

PpsbAI::luxCDE

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAM2195

Plasmid

#40245

Depositor

Susan Golden

Article

Mackey et al Methods Mol Biol. 2007;362:115-29.

Insert

PpsbAI::luxCDE, PpsbAI::luxAB

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pPS1303

Plasmid

#8849

Depositor

Pamela Silver

Article

Shen et al Genes Dev 1998 Mar 1;12(5):679-91.

Insert

Gal-sGFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pSR43.6r

Plasmid

#63197

Purpose

Expresses CcaS, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLV_Stargazin-mTurquoise2-iLID

Plasmid

#161001

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Stargazin-mTurquoise2-iLID

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_T7-ARG1

Plasmid

#106473

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBWB162

Plasmid

#140634

Purpose

pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

mCherry

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5

Plasmid

#124362

Purpose

Overexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-F30-Broccoli-aptNFkB#5

Expression

Mammalian

Promoter

U6+27

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
miniCMV-(mNeonGreen)4-tDeg

Plasmid

#129402

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)-tDeg

Expression

Mammalian

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR33.4r

Plasmid

#63198

Purpose

Expresses Cph8, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

Cph8
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4 Smad4

Plasmid

#16483

Depositor

Bert Vogelstein

Article

Riggins et al Nat Genet. 1996 Jul . 13(3):347-9.

Insert

Smad4 (SMAD4 Human)

Tags

Double HA

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pSR58.6

Plasmid

#63176

Purpose

Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMUH_unc84_2XGFP

Plasmid

#46023

Depositor

Sean Eddy

Article

Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

Insert

UNC-84 (unc-84 )

Use

10xuas-phi31-expression vector

Tags

2XGFP

Mutation

Two amino acid mutations (F279L and L500P) compar…

Promoter

10XUAS

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
UbC-(mNeonGreen)4-tDeg

Plasmid

#129404

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)4-tDeg

Expression

Mammalian

Promoter

UbC

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWL89A mPing

Plasmid

#145787

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock, Susan Wessler

Article

Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

Insert

mPing

Expression

Yeast

Mutation

WT

Available Since

Jan. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLV_mTurquoise2-iLID-CAAX

Plasmid

#160999

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRAS

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

mTurquoise2-iLID-CAAX

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIgnaviCas9

Plasmid

#127595

Purpose

Expresses IgnaviCas9 in E. coli

Depositor

Stephen Quake

Article

Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

Insert

IgnaviCas9

Tags

6xHis Tag and Maltose binding protein

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMUH_unc84_tdTFlag

Plasmid

#46024

Depositor

Sean Eddy

Article

Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

Insert

UNC-84 (unc-84 )

Use

10xuas-phi31-expression vector

Tags

tdTomato

Mutation

Two amino acid mutations (F279L and L500P) compar…

Promoter

10XUAS

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
ZipGFP11_TEV

Plasmid

#81243

Purpose

Zipped GFP11 with TEV cleavage sequence

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

zipped GFP11 with TEV cleavage sequence

Expression

Mammalian

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-5S-Squash

Plasmid

#177914

Purpose

Expresses 5S rRNA fused to Squash aptamer in mammalian cells

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Squash aptamer

Expression

Mammalian

Promoter

5S

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBWB294

Plasmid

#140636

Purpose

pBAV1k-lacI-Trc-BCD9-mCherry. BCD RBS.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

mCherry

Expression

Bacterial

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-HA-hTAF4b

Plasmid

#17998

Depositor

Robert Tjian

Article

Freiman et al Science. 2001 Sep 14. 293(5537):2084-7.

Insert

hTAF4b (TAF4B Human)

Tags

HA

Expression

Mammalian

Mutation

aa1-64 deleted

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only

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