167,924 results

1. SepRS(2)/pSertRNA(B4)/EF-Sep

   Plasmid

   #173897

   Purpose

   The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

   Depositor

   Jason W Chin

   Article

   Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

   Inserts

   SepRS(2)
   pSertRNA
   EF-Sep
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-C1-PLCdelta-PH

   Plasmid

   #21179

   Purpose

   encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

   Depositor

   Tobias Meyer

   Article

   Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

   Insert

   PLCdelta (PLCD1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PH domain of human PLC delta, amino acids Met1–Il…

   Available Since

   July 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTYB1-eIF4A

   Plasmid

   #37225

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Insert

   eIF4A
   

   Tags

   intein

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-S33Y Beta-catenin

   Plasmid

   #19286

   Purpose

   Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S33Y (presumptive GSK3β phosphorylation site)

   Available Since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJBL7079

   Plasmid

   #167244

   Purpose

   cell-free lac sensor with GFP output

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pLac-sfGFP
   

   Use

   Synthetic Biology

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJBL7080

   Plasmid

   #167245

   Purpose

   cell-free lac sensor with RFP output

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pLac-mRFP1
   

   Use

   Synthetic Biology

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJBL7081

   Plasmid

   #167246

   Purpose

   cell-free lac sensor with Mango output

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pLac-Mango
   

   Use

   Synthetic Biology

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBL7082

   Plasmid

   #167247

   Purpose

   cell-free lac sensor with XylE output

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pLac-XylE
   

   Use

   Synthetic Biology

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJBL7086

   Plasmid

   #167250

   Purpose

   orthogonal Mango reporter output for cell-free expression

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pAKSIRV-Mango
   

   Use

   Synthetic Biology

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pR6K-GFP-CASTIF

    Plasmid

    #199653

    Purpose

    R6K plasmid encoding a type I-F CAST but no CRISPR array

    Depositor

    IIya Finkelstein

    Insert

    CAST I-F systems
    

    Expression

    Bacterial

    Promoter

    J23119 promoter

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pClodANL-FGF2

    Plasmid

    #107871

    Purpose

    Bacterial expression of FGF2-NanoLuc

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Insert

    FGF2
    

    Use

    Luciferase

    Tags

    NanoLuc

    Expression

    Bacterial

    Mutation

    wt sequence

    Promoter

    pro D

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV_IRES_GFP_CD16a

    Plasmid

    #196189

    Purpose

    Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

    Depositor

    Ana Ruiz-Saenz

    Article

    Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

    Insert

    CD16a (FCGR3A Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 158 to Valine

    Promoter

    LTR

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-GRAB-rDA2m

    Plasmid

    #208700

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA2m
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

    Plasmid

    #140534

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-NLS TOL2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 PRDM16

    Plasmid

    #15503

    Depositor

    Bruce Spiegelman

    Article

    Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

    Insert

    PRDM16 (Prdm16 Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMBP-LbCas12a

    Plasmid

    #113431

    Purpose

    Expresses 10xHis-MBP fused to LbCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Has Service

    Cloning Grade DNA

    Insert

    LbCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.GfaABC1D.PI.Lck-GFP.SV40 (AAV5)

    Viral Prep

    #105598-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV.GfaABC1D.PI.Lck-GFP.SV40 (#105598). In addition to the viral particles, you will also receive purified pAAV.GfaABC1D.PI.Lck-GFP.SV40 plasmid DNA. GfaABC1D-driven GFP expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al J Gen Physiol. 2013 Apr 15.

    Promoter

    GfaABC1D

    Tags

    GFP

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pGP-CMV-NES-jRGECO1a

    Plasmid

    #61563

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRGECO1a
    

    Tags

    nuclear export signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-SRRM2-mCherry

    Plasmid

    #174089

    Purpose

    Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

    Depositor

    Katie Ullman, Wesley Sundquist

    Article

    Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

    Insert

    SRRM2 (SRRM2 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TREtight

    Available Since

    Sept. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP VAMP8

    Plasmid

    #42311

    Depositor

    Thierry Galli

    Article

    Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

    Insert

    VAMP 8 (VAMP8 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

    Plasmid

    #223747

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    Lambda-PPase
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1-2xFLAG-SREBP-1c

    Plasmid

    #26802

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    SREBP-1c (SREBF1 Human)
    

    Tags

    2XFLAG

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLVX_DSB_Spectrum-V1

    Plasmid

    #192474

    Purpose

    Lentiviral DSB Spectrum V1 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, incompleteGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLV-hSyn-RFP

    Plasmid

    #22909

    Depositor

    Edward Callaway

    Article

    Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

    Has Service

    Cloning Grade DNA

    Insert

    human synapsin I promoter driving DsRedExpress (SYN1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcs2-GFP-IRES-mCherry

    Plasmid

    #226098

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pGL4.27_ARE/NRF2-SPE

    Plasmid

    #177775

    Purpose

    Luciferase reporter for ARE/NRF2

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    Luciferase

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid

    #101893

    Purpose

    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Promoter

    CMV immediate early

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. Anti-amyloid-β 40/42 nanobody (R3VQ) with sortase tag

    Plasmid

    #233217

    Purpose

    Mammalian epression of anti-amyloid-β 40/42 nanobody (R3VQ) with a sortase tag for direct dye conjugation.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv 2023.10.24.563674

    Insert

    Anti-amyloid-β 40/42 nanobody (R3VQ)
    

    Tags

    Sortase tag, His tag

    Expression

    Mammalian

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Douglas Kim, GENIE Project, Loren Looger

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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30. PCS2+Cas9-mSA

    Plasmid

    #103882

    Purpose

    Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    CAS9-MSA
    

    Use

    CRISPR

    Tags

    MSA (monomeric streptavidin

    Mutation

    none

    Promoter

    SP6

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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31. pEGFP-N1-FLAG

    Plasmid

    #60360

    Purpose

    Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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32. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV9)

    Viral Prep

    #162381-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    CAG

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

33. MSCV-IRES-GFP

    Plasmid

    #20672

    Purpose

    Depositor

    Tannishtha Reya

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES EGFP

    Expression

    Mammalian

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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34. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid

    #73497

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB-P2A-mCherry
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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35. AAV phSyn1(S)-FlpO-bGHpA (AAV Retrograde)

    Viral Prep

    #51669-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from AAV phSyn1(S)-FlpO-bGHpA (#51669). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-FlpO-bGHpA plasmid DNA. hSyn-driven FlpO expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Promoter

    Syn

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

36. pRL-SV40P

    Plasmid

    #27163

    Purpose

    Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments

    Depositor

    Ron Prywes

    Article

    Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702

    Insert

    SV40 promoter
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYL192 (TRV1)

    Plasmid

    #148968

    Purpose

    Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    TRV RNA1
    

    Use

    Plant vector with trv rna1; this vector used with…

    Mutation

    P697S mutation in putative replicase. S239G and S…

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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38. [#GS5-RV] MitoTRACER-Donor

    Plasmid

    #233505

    Purpose

    Retroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    MitoTRACER-Donor
    

    Use

    Retroviral and Synthetic Biology

    Tags

    GFP11 and HA Tag

    Expression

    Mammalian

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1

    Plasmid

    #138461

    Purpose

    lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    MCP-VP64-IRES-zsGreen1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SpCas9-YE1-BE4max

    Plasmid

    #198546

    Purpose

    Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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