We narrowed to 59,431 results for: plasmid
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Plasmid#141260PurposeGateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pcDNA3.1-hACE2
Plasmid#145033PurposeExpress human ACE2 with C9 tag at C-terminal in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman ACE2 (ACE2 Human)
TagsC9ExpressionMammalianPromoterCMVAvailable sinceApril 15, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pAAV:cTNT::Luciferase
Plasmid#69915PurposeUsed to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.DepositorInsertFirefly luciferase
UseAAVPromoterChicken cardiac troponin TAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CMV-GFP
Plasmid#67634PurposeAAV vector expressing GFPDepositorInsertGFP
UseAAVPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CRE recombinase
Plasmid#62730PurposeExpresses Cre recombinase protein in bacterial cellsDepositorInsertCre recombinase
UseCre/LoxTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-flex-taCasp3-TEVp
Plasmid#45580DepositorHas serviceAAV1, AAV5, AAV8, and AAV RetrogradeInsertsUseAAV; Adeno associated viral vectorMutationLinker replaced with a TEV protease cleavage sitePromoterEF1aAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pL-CRISPR.EFS.tRFP
Plasmid#57819PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter drivenDepositorAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330S-2
Plasmid#58778PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUAS-luc2
Plasmid#24343DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuciferase
Tags5XGAL4ExpressionMammalianAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFLP3
Plasmid#64946PurposeFlp recombinase expression vectorDepositorPublicationInsertFlp recombinase
ExpressionBacterialPromoterlambda promoter/temperature-sensitive Lambda cI r…Available sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pLSC-5
Plasmid#62889PurposeLenti vector for expression of inducible split Cas9DepositorInsertsSpCas9(574-1368)
SpCas9(2-573)
UseCRISPR and LentiviralTagsFFBP12, FRB, NES PTK2, NLS SV40, and P2AExpressionMammalianMutation*see comment belowPromoterEFSAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEN-luxCDABE
Plasmid#44918DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsigma 70 promoter
UseLuciferaseTagslux transcriptional fusionPromoterem7Available sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO5.sgRNA.EFS.tRFP
Plasmid#57823Purpose3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP
UseCRISPR and LentiviralAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223 SARS-CoV-2 NSP16
Plasmid#141269PurposeGateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr
Plasmid#19326DepositorPublicationInsertPmyo-3::twk-18(cn110)cDNA
ExpressionWormAvailable sinceOct. 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCFJ178 - cxTi10882_MCS
Plasmid#19331DepositorPublicationInsertcxTi10882 targetting region
ExpressionWormAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE
Plasmid#138147PurposeExpresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInsertCasRx
UseLentiviralTagsNLSAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJR89
Plasmid#140096PurposesgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.DepositorInsertsgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
Available sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKIIa-mCherry
Plasmid#114469PurposemCherry ControlDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV RetrogradeInsertmCherry
UseAAVPromoterCaMKIIaAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by