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175,655 results

Showing: 3651 - 3700 of 175655 results

  1. Venus-79aa-ActA-pEGFP-C1

    Plasmid
    #166763
    Purpose
    To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    ActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166763-plasmid-map-sequence-id-328851

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  3. LentiGuide_DKO

    Plasmid
    #183193
    Purpose
    Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available Since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183193-plasmid-map-sequence-id-360099

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  5. pAAV.TBG.PI.eGFP.WPRE.bGH

    Plasmid
    #105535
    Purpose
    AAV expression of EGFP from TBG promoter
    Depositor
    James M. Wilson
    Has Service
    AAV8
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    TBG
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105535-plasmid-map-sequence-id-202369

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  7. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  10. pVITRO1-Trastuzumab-IgG1/κ

    Plasmid
    #61883
    Purpose
    Expresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available Since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61883-plasmid-map-sequence-id-358014

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  15. FLT3-ITD-MSCV-IRES-GFP

    Plasmid
    #184779
    Purpose
    Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells
    Depositor
    Timothy Ley
    Insert
    FLT3 (FLT3 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    Activating internal tandem duplication (ITD) adde…
    Promoter
    MSCV
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184779-plasmid-map-sequence-id-364900

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  17. pAAV-hSyn Con/Fon EYFP (AAV8)

    Viral Prep
    #55650-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn Con/Fon EYFP (#55650). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon EYFP plasmid DNA. Synapsin driven, Cre and Flp-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    Syn
    Tags
    EYFP (Cre- and Flp-dependent)
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  21. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  22. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  23. pLenti-EF1a-Luciferase-IRES-Blast-WPRE

    Plasmid
    #108542
    Purpose
    Lentivirus vector expressing Luciferase with blasticidin resistance
    Depositor
    Javier Alcudia
    Inserts
    Luciferase
    Neomycin
    Use
    Lentiviral
    Promoter
    EF1apha and PGK
    Available Since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    108542-plasmid-map-sequence-id-208671

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  24. pAAV-hSyn-Cre-P2A-dTomato (AAV2)

    Viral Prep
    #107738-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Rylan Larsen
    Promoter
    Syn
    Tags
    dTomato (physically separate, not a fusion protein)
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only

  25. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 (AAV1)

    Viral Prep
    #101060-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 (#101060). In addition to the viral particles, you will also receive purified pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator his-tagged CaMPARI2. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Eric Schreiter
    Article
    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:
    Promoter
    Syn
    Available Since
    July 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  27. pIVM_02

    Plasmid
    #204500
    Purpose
    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)
    Depositor
    Alessio Ciulli
    Article
    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.
    Insert
    amino acids 54-213 VHL (VHL Human)
    Tags
    6xHis, TEV, and Thrombin
    Expression
    Bacterial
    Mutation
    0
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204500-plasmid-map-sequence-id-406419

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  28. AAV-mDlx-jGCaMP8f-WPRE (AAV1)

    Viral Prep
    #176753-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-mDlx-jGCaMP8f-WPRE (#176753). In addition to the viral particles, you will also receive purified AAV-mDlx-jGCaMP8f-WPRE plasmid DNA. mDlx-driven expression of ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    mDlx
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  30. pAAV-Ef1a-fDIO-GCaMP6s (AAV8)

    Viral Prep
    #105714-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO-GCaMP6s (#105714). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-GCaMP6s plasmid DNA. EF1a-driven, Flp recombinase-dependent expression of GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals
    Depositor
    Rylan Larsen
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Available Since
    Oct. 11, 2019
    Availability
    Academic Institutions and Nonprofits only

  37. epi-BE4max-NG

    Plasmid
    #135977
    Purpose
    Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
    Depositor
    Yongming Wang
    Article
    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only

  39. pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA (AAV9)

    Viral Prep
    #20299-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA (#20299). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent mCherry expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Tags
    mCherry (Cre-dependent)
    Available Since
    Sept. 22, 2025
    Availability
    Academic Institutions and Nonprofits only

  45. pMS07

    Plasmid
    #230070
    Purpose
    Inducible Promoter (rhamnose-induced, BT3763/rhaR system), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT
    Depositor
    Sean Crosson
    Article
    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Bacterial
    Promoter
    rhamnose-inducible (BT3763)
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    230070-plasmid-map-sequence-id-486619

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  46. pMS03

    Plasmid
    #230937
    Purpose
    Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT
    Depositor
    Sean Crosson
    Article
    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Bacterial
    Promoter
    RpoD (bt_1311/BT_RS06635)
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    230937-plasmid-map-sequence-id-476750

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  47. pMS05

    Plasmid
    #230069
    Purpose
    Inducible Promoter (anhydrotetracycline-induced, tetO/tetR system), replicates via RepA; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT
    Depositor
    Sean Crosson
    Article
    Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Bacterial
    Promoter
    anhydrotetracycline-inducible
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    230069-plasmid-map-sequence-id-476753

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  49. AAV-CamKIIa-jGCaMP8m-WPRE (AAV5)

    Viral Prep
    #176751-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CamKIIalpha
    Available Since
    Jan. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 3651 - 3700 of 175655 results