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Showing: 3651 - 3700 of 163983 results

  4. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (AAV9)

    Viral Prep
    #124603-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (#124603). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 plasmid DNA. EF1a-driven, Cre-dependent expression of ChrimsonR fused to mRuby2 fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Christopher Harvey
    Article
    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-
    Promoter
    Tags
    mRuby2 (Cre-dependent)
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV-hSyn-hChR2(H134R)-EYFP

    Plasmid
    #26973
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26973-plasmid-map-sequence-id-199313

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  9. pENN.AAV.CamKII 0.4.Cre.SV40 (AAV1)

    Viral Prep
    #105558-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CaMKII
    Tags
    None
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. pLKO.1 hygro

    Plasmid
    #24150
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
    Depositor
    Bob Weinberg
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for shRNA
    Available since
    March 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    24150-plasmid-map-sequence-id-376525

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  16. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Plasmid
    #154430
    Purpose
    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    Depositor
    David Liu
    Article
    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:
    Insert
    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    VRQR point mutations in SpCas9
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    154430-plasmid-map-sequence-id-304586

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  19. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt (AAV1)

    Viral Prep
    #175181-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt (#175181). In addition to the viral particles, you will also receive purified pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt plasmid DNA. Synapsin-driven, Cre-dependent, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Promoter
    Syn
    Tags
    None
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only

  20. HA-Rab7A

    Plasmid
    #131417
    Purpose
    Expresses HA-tagged human Rab7A in mammalian cells
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Insert
    human Rab7A
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131417-plasmid-map-sequence-id-258194

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  22. pENN.AAV.CamKII0.4.eGFP.WPRE.rBG (AAV5)

    Viral Prep
    #105541-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pENN.AAV.CamKII0.4.eGFP.WPRE.rBG (#105541). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII0.4.eGFP.WPRE.rBG plasmid DNA. Expression of EGFP from CamKII promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CaMKII
    Tags
    EGFP
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only

  25. pET28a_DmKHC[1-421]-1xGFP-6xHis

    Plasmid
    #196972
    Purpose
    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag
    Depositor
    Lukas Kapitein
    Article
    Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    6xHis and GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196972-plasmid-map-sequence-id-388918

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  26. V. natriegens NC7

    Bacterial Strain
    #215356
    Purpose
    Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.
    Depositor
    Buz Barstow
    Bacterial Resistance
    None
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only

  28. pMIBlu

    Plasmid
    #176621
    Purpose
    Retroviral transfer vector expressing TagBFP
    Depositor
    Nevil Singh
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    176621-plasmid-map-sequence-id-358076

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  29. ADRA1B-Tango

    Plasmid
    #66214
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA1B (ADRA1B Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66214-plasmid-map-sequence-id-157894

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  30. Negative Control Binder

    Plasmid
    #179140
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptide
    Depositor
    Klaus Hahn
    Article
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Insert
    SspB
    Use
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9. Addi…
    Promoter
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179140-plasmid-map-sequence-id-353410

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  37. KHK

    Plasmid
    #25532
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KHK (KHK Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. pdCas9-bacteria

    Plasmid
    #44249
    Purpose
    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 (bacteria)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    pLtetO-1
    Available since
    April 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    44249-plasmid-map-sequence-id-269588

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  41. GRP94-COMP-blac-flag-his

    Plasmid
    #111008
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    endoplasmin, putative (GRP94) (PFL1070c Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111008-plasmid-map-sequence-id-224562

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  42. ICAM5-bio-His

    Plasmid
    #51759
    Purpose
    Expresses full-length Intercellular adhesion molecule 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ICAM5 (ICAM5 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51759-plasmid-map-sequence-id-79348

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  44. hSyn-DIO-somBiPOLES-mCerulean (AAV9)

    Viral Prep
    #154951-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Simon Wiegert
    Article
    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:
    Promoter
    Syn
    Tags
    mCerulean (Cre-dependent)
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only

  47. pUBC_HA_2xPP7-VP64

    Plasmid
    #68418
    Purpose
    Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    PP7(deltaFG)
    Use
    Synthetic Biology
    Tags
    HA and VP64
    Expression
    Mammalian
    Mutation
    PP7: [delta]67_74 (non-aggregating)
    Promoter
    human UbC
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68418-plasmid-map-sequence-id-125550

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Showing: 3651 - 3700 of 163983 results