-
Plasmid#128211Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH51
Plasmid#128213Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPJ173(JPUB_006777)
Plasmid#83834PurposeCarries NEW operon 10 finalDepositorInsertsKuste3336
Kuste3339
Kuste3341
UseTagsExpressionMutationPromoterop10_3339_for, op10_3341_for, and op10_BsaI_3336_…Available sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPJ167(JPUB_006765)
Plasmid#83831PurposeCarries NEW operon 3 finalDepositorInsertsKuste2803
Kuste2804
UseTagsExpressionMutationPromoterop3_2804_for and op3_BsmBI_2803_forAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
S7_SwaI_Zeo_PacI
Plasmid#128471PurposeZeo Resistance geneDepositorInsertZeocin Resistance gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH71
Plasmid#128214Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-eGFP-Esp3I
Plasmid#81226PurposeCloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sitesDepositorInsertEsp31-Neo-Esp31 deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCHW00010
Plasmid#222435PurposeLevel 0, promoter module (GGAG-AATG) containing CBS4 and tomato miniDFR promoters.DepositorInsertCBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3225
Plasmid#234087PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertDasherGFP
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3234
Plasmid#234106PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertaeBlue
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3223
Plasmid#234085PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInserteforCP
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3229
Plasmid#234101PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInserteforCP
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3231
Plasmid#234103PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertDasherGFP
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEED-Moontag:HA
Plasmid#219648PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest methodDepositorInsertMoonTag:HA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLigify_dRibose
Plasmid#215561PurposeExpresses GFP in response to D-RiboseDepositorInsertsGFP mut2
LcLacI
UseTagsExpressionBacterialMutationPromoterP250 and PlclaciAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAXY0010
Plasmid#205579PurposeKAN(1-192)-NpuDnaE(N)_GFPuvDepositorInsertNpuDnaE(N)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0008
Plasmid#205577PurposeKAN(1-131)-NpuDnaE(N)_GFPuvDepositorInsertNpuDnaE(N)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH50
Plasmid#128212Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK007
Plasmid#128220Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH72
Plasmid#128221Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only