Addgene

We narrowed to 25,874 results for: Mpl;

Showing: 3001 - 3040 of 25874 results

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pPS1889

Plasmid

#1361

Depositor

Pamela Silver

Article

Damelin et al Mol Cell 2000 Jan;5(1):133-40.

Insert

NLS-ECFP-EYFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pET-6xHis-GFP(-7)-neg_tag1

Plasmid

#219419

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

GFP(-7)-neg_tag1

Use

Tags

DEEEDD and MGHHHHHGG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-7xHA-PEX6-Tomato

Plasmid

#183731

Purpose

Expression of human 7xHA- PEX6 fused to Tomato, and PEX6 3'UTR

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Insert

PEX6-Tomato-PEX63'UTR

Use

Lentiviral

Tags

7xHA

Expression

Mutation

Promoter

UbiC

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAM238

Plasmid

#226639

Purpose

S. cerevisiae ubiquitin

Depositor

Andreas Martin

Article

Lander et al Nature. 2012 Jan 11;482(7384):186-91. do

Insert

Ubiquitin (UBI4 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-mBlueberry(-9)

Plasmid

#219416

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

mBlueberry2(-9)

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

K137E, K199E, R234E

Promoter

T7

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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T5-pAc5.1 EGFP-EcoRV

Plasmid

#206481

Purpose

Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Type

Empty backbone

Use

Tags

EGFP

Expression

Insect

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIC-HIS10-ZBASIC-TEV-GG

Plasmid

#215797

Purpose

Cloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage

Depositor

Frank von Delft, ASAP Discovery

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pNIC- AVITAG-CTH10STII- -GG

Plasmid

#215805

Purpose

Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

Depositor

Frank von Delft, ASAP Discovery

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
T8-pAc5.1 lambdaN HA EcoRV

Plasmid

#206492

Purpose

Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MTF1

Plasmid

#205066

Purpose

MTF1 protein expression

Depositor

Smita Patel

Article

Tang et al J Biol Chem. 2009 Feb 27;284(9):5514-22.

Insert

MTF1 (MTF1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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LDB59

Plasmid

#185436

Purpose

NUP1 CEN URA3 plasmid

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCSTG4FER3(#190)

Plasmid

#184069

Purpose

cyclofen-inducible GAL4 activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Insert

Gal4bdFF-ERT2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

sCMV+SP6

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-PPP2R5A-1

Plasmid

#179352

Purpose

shRNA suppression

Depositor

William Hahn

Article

Sablina et al Cancer Res. 2010 Dec 15;70(24):10474-84.

Insert

B56α

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Age1

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-PPP2R5C-2

Plasmid

#179356

Purpose

shRNA suppression

Depositor

William Hahn

Article

Sablina et al Cancer Res. 2010 Dec 15;70(24):10474-84.

Insert

B56γ

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Age1

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-PPP2R3B-1

Plasmid

#179360

Purpose

shRNA suppression

Depositor

William Hahn

Article

Sablina et al Cancer Res. 2010 Dec 15;70(24):10474-84.

Insert

PR48

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Age1

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG07-pLac-NRII_L16R

Plasmid

#102464

Purpose

Expresses NRII protein with L16R mutation in E. coli. The expression is controlled by a pLac promoter.

Depositor

Richard Murray

Article

Guo et al bioRxiv 122606

Insert

NRII (ntrB )

Use

Tags

Expression

Bacterial

Mutation

Leucine 16 to Arginine

Promoter

pLac

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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SL-150-0

Plasmid

#108677

Purpose

in vitro mRNA transcription

Depositor

Jon Lorsch

Article

Yourik et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

CAA repeat mRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRL648

Plasmid

#70283

Purpose

pUC plasmid with inverted polylinker flanking PpsbA-Km marker

Depositor

Peter Wolk

Article

Black et al J Bacteriol. 1994 Apr;176(8):2282-92.

Type

Empty backbone

Use

Bacterial shuttle vector

Tags

Expression

Mutation

Promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pNJB98

Plasmid

#51520

Purpose

Gateway entry vector with attL5 and attL2 sites flanking us:NPTII repair template sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

us:nptII repair template

Use

Tags

Expression

Mutation

Promoter

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pHAC111-LST8 (pSW75)

Plasmid

#39211

Depositor

Michael Hall

Article

Wullschleger et al J Biol Chem. 2005 Sep 2;280(35):30697-70

Insert

LST8 (LST8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

LST8

Available since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
pBN27

Plasmid

#35120

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1delta1

Use

Yeast replicating

Tags

Expression

Mutation

amplicon has a complete deletion of FCY1 ORF, fla…

Promoter

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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ylqF-pET21a

Plasmid

#30133

Depositor

SeWon Suh

Article

Kim do et al Proteins. 2008 Sep . 72(4):1363-70.

Insert

ylqF

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pAL-SK-swi3+

Plasmid

#15982

Depositor

Eishi Noguchi

Article

Noguchi et al Mol Cell Biol. 2004 Oct . 24(19):8342-55

Insert

Swi3 (swi3 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only
pLX-EF1a_GFP_CdTag-HA

Plasmid

#221492

Purpose

GFP lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30ax

Plasmid

#85761

Purpose

A bacterial expression vector with a His-tag at N and C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Use

Tags

His tag (N-term and C-term)

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFBDM

Plasmid

#110738

Purpose

Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin and p10 promoters

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDestTol2pA2_ubi:EGFP

Plasmid

#27323

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:EGFP reporter

Use

Tol2 transposon

Tags

Expression

Mutation

Promoter

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINE GFP nanobody

Plasmid

#49172

Purpose

Bacterial expression of GFP nano body

Depositor

Brett Collins

Article

Kubala et al Protein Sci. 2010 Dec;19(12):2389-401. d

Insert

GFP nanobody

Use

Tags

HHHHHH

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPG

Plasmid

#71248

Purpose

improved luciferase reporter gene plasmid

Depositor

Peter Cockerill

Article

Bert et al Plasmid. 2000 Sep;44(2):173-82.

Type

Empty backbone

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

none

Available since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3 RARE-RFP

Plasmid

#183054

Purpose

Fluorescent reporter for Retinoic Acid activity

Depositor

Chaya Kalcheim

Article

Rekler et al Elife. 2022 Apr 8;11. pii: 72723. doi: 1

Insert

RFP

Use

Tags

Expression

Mutation

Promoter

RARE, SV40

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-hCRM1

Plasmid

#17647

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

CRM1 (XPO1 Human)

Use

Tags

3x Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pNICE-NL2A

Plasmid

#15259

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 2 with insert A (Nlgn2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR/D_creERt2

Plasmid

#27321

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

creERt2

Use

Multisite gateway middle entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pT26CfpSox10G4FER3(#1048)

Plasmid

#184073

Purpose

cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Insert

Gal4bdFF-ERT2

Use

Zebrafish transgenesis (blue heart)

Tags

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Sox10

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiFC-bFosVC155

Plasmid

#22013

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bFosVC155

Use

Tags

HA

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18T-mini-Tn7T-Zeo-gfpmut3

Plasmid

#65037

Purpose

ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

gfpmut3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pIIIA/MS2-1

Plasmid

#220631

Purpose

For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIIIA/MS2-2

Plasmid

#220632

Purpose

For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS7

Plasmid

#32056

Purpose

Vector optimized for constitutive gene expression in Burkholderia genus and related bacteria

Depositor

Miguel Valvano

Article

Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ps7 promoter, originated from Paraburkholderia xe…

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCE-BiFC-VN173

Plasmid

#22019

Depositor

Chang-Deng Hu

Article

Shyu et al Nat Protoc. 2008 . 3(4):588-96.

Insert

VN173

Use

Tags

Myc

Expression

Worm

Mutation

N/A

Promoter

Available since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3001 - 3040 of 25874 results

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