We narrowed to 5,642 results for: pcas

1. pCas9

   Plasmid

   #162606

   Purpose

   Pre-express Cas9 in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas34

   Plasmid

   #82386

   Purpose

   sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas3

   Plasmid

   #229538

   Purpose

   pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Insert

   Cas3
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT007

   Plasmid

   #171634

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGTetRnls

   Plasmid

   #26599

   Depositor

   Peter Andrews

   Article

   Zafarana et al Stem Cells. 2009 Apr . 27(4):776-82.

   Insert

   Tetracycline repressor (TetR E.Coli)
   

   Use

   Tags

   nls

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAH01

   Plasmid

   #128162

   Purpose

   IncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteria

   Depositor

   Calvin Henard, Michael Guarnieri

   Article

   Henard et al Sci Rep. 2016 Feb 23;6:21585. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAT220

   Plasmid

   #134888

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCast

   Plasmid

   #200169

   Purpose

   E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

   Depositor

   Yong-Su Jin

   Article

   Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCaRNA1&2

   Plasmid

   #86087

   Purpose

   Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

   Depositor

   Aiming Wang

   Article

   Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

   Insert

   RNA1, RNA2
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCaRNA3

    Plasmid

    #86088

    Purpose

    Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cells

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    RNA3
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAT202

    Plasmid

    #134879

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    deletion of Y208

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCasX1

    Plasmid

    #87685

    Purpose

    p15A plasmid expressing CasX locus from Deltaproteobacteria

    Depositor

    Jennifer Doudna

    Article

    Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

    Insert

    CasX1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas9CR5-NG

    Plasmid

    #120228

    Purpose

    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.

    Depositor

    Christopher Reisch

    Insert

    SpCas9-NG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

    Promoter

    pTet

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAT105

    Plasmid

    #180510

    Purpose

    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    DpbCasX with R3 loop
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS_2paCRY2

    Plasmid

    #129651

    Purpose

    2P-activatable CRY2

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG:ECFP

    Plasmid

    #32597

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

    Insert

    ECFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCA_e-2

    Plasmid

    #202097

    Purpose

    Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCas_2+5

    Plasmid

    #60594

    Purpose

    encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Cas_2+5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCARSF63

    Plasmid

    #64695

    Purpose

    H6-TRX-Smt3-GB1-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Use

    Tags

    CBD, HisTag, NpuDnaE-N102, Smt3, and TRX

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGmRuby2smFPMyc

    Plasmid

    #71816

    Purpose

    encodes multiple copies of an epitope tag Myc ( not detected by anti GFP ab)

    Depositor

    Loren Looger

    Insert

    mRuby2_smFP_Myc
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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