We narrowed to 5,839 results for: pCas

1. pCAG-Sox2-IRES-Neo

   Plasmid

   #13462

   Depositor

   Shinya Yamanaka

   Article

   Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

   Insert

   Sox2 (Sox2 Mouse)
   

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-GBP1-10gly-lexADBD

   Plasmid

   #50795

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP1-lexA DNA binding domain fusion protein
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-hPhyBY276H-mCherry-HRasCT

   Plasmid

   #100540

   Purpose

   Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)
   

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-908 aa), Y276H muta…

   Promoter

   CAG promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKRG3-mU6-PUb-hSpCas9

   Plasmid

   #162162

   Purpose

   Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   N/A

   Expression

   Insect

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-mTagBFP2-T2A-FLPo

   Plasmid

   #124834

   Purpose

   Monomeric TagBFP2 and FLP recombinase

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-FLPo
   

   Expression

   Mammalian

   Available Since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-SpCas9D10A nickase

   Plasmid

   #216737

   Purpose

   Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CMVd1

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBT346_pLambda-attB-pCA-GFP-pA

   Plasmid

   #52551

   Purpose

   for producing PhiC31 attB-containing minicircle in vitro

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   Lambda integrase attL1
   Lambda integrase attR1
   PhiC31o attB (full length)
   GFP
   

   Expression

   Mammalian

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9/VRQR-sgRNA (BbsI)

   Plasmid

   #129725

   Purpose

   Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-HA-rat Nrxn1beta AS4(-)

   Plasmid

   #58267

   Purpose

   Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

   Depositor

   Peter Scheiffele

   Article

   Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

   Insert

   Nrxn1beta AS4(-) (Nrxn1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   see comments

   Promoter

   CAG

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-mEGFP-CaMKIIa (T286A)

    Plasmid

    #127390

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Alanine

    Promoter

    pCAG

    Available Since

    Jan. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas-pML-1B_6xHis-Cas12m

    Plasmid

    #192282

    Purpose

    MmCas12m fused to His-tag for protein purification

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmCas12m
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pspCas9-HF-tetra-com-vector

    Plasmid

    #132555

    Purpose

    Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    High fidelity Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Expression

    Mammalian

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9-NG-APOBEC-nCas9-Ung

    Plasmid

    #198539

    Purpose

    Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC0043-PspCas13b-gRNA LINE1

    Plasmid

    #223699

    Purpose

    gRNA of dPspCas13b-FTO targeting LINE1

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    gRNA target sequence for LINE1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCambia-dLB-kanR-GFP

    Plasmid

    #223177

    Purpose

    Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border

    Depositor

    Andreas Stuermer

    Type

    Empty backbone

    Use

    Ti plasmid for agrobacterium

    Expression

    Plant

    Available Since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCAMBIA-FLS2-myc-mCherry

    Plasmid

    #86159

    Purpose

    FLS2-mCherry expression

    Depositor

    Silke Robatzek

    Article

    Mbengue et al Proc Natl Acad Sci U S A. 2016 Sep 27;11

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Tags

    mCherry and myc

    Expression

    Plant

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-TET3G-T2A-Puro

    Plasmid

    #158067

    Purpose

    Encodes the Tet3G transactivator and puromycin resistance.

    Depositor

    Adolfo Rivero-Muller

    Article

    Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

    Insert

    pCAG-TET3G-T2A-Puro
    

    Use

    AAV

    Tags

    puromycin reistance gene

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-FIP200(1-640)-MBP

    Plasmid

    #203543

    Purpose

    FIP200 NTD-GFP mammalian expression

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Tags

    MBP-10His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCA24N-ligase-NLS-cTF

    Plasmid

    #87746

    Purpose

    IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis and NLS linker - cTF (chimeric transcriptio…

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9-EcRT-GAL-Hyg

    Plasmid

    #232091

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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