Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,672 results for: pCas

Clear keywords
Showing: 1621 - 1640 of 2672 results

  1. PX458-sgTSC2

    Plasmid
    #128098
    Purpose
    CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP
    Depositor
    Chi Van Dang
    Publication
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against human TSC2 (TSC2 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128098-plasmid-map-sequence-id-249673

    Enlarge

  2. pRS0045

    Plasmid
    #131124
    Purpose
    Mammalian Target-ACE expression vector
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    Use
    CRISPR
    Tags
    PmCDA1, TadA, and UGI
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131124-plasmid-map-sequence-id-257454

    Enlarge

  4. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

    Plasmid
    #135012
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135012-plasmid-map-sequence-id-267596

    Enlarge

  6. pxCas9CR4

    Plasmid
    #111656
    Purpose
    PCas9CR4 with the xCas9 mutations allowing NG PAM sites
    Depositor
    Christopher Reisch
    Insert
    TetR and xCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Bacterial
    Mutation
    xCas9 mutations
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111656-plasmid-map-sequence-id-217189

    Enlarge

  8. YE1-BE4max-CP1028

    Plasmid
    #138160
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    138160-plasmid-map-sequence-id-272345

    Enlarge

  12. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135014-plasmid-map-sequence-id-267599

    Enlarge

  13. px330 VRER

    Plasmid
    #101729
    Purpose
    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VRER
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VRER (D1135V, G1218R, R1335E, and T1337R mutation…
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101729-plasmid-map-sequence-id-265859

    Enlarge

  15. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #129418
    Purpose
    Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus
    Depositor
    Elina Ikonen
    Publication
    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 and sgRAB18.N (RAB18 Human)
    Use
    CRISPR
    Tags
    T2A-mCherry-P2A-Puro
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129418-plasmid-map-sequence-id-254820

    Enlarge

  17. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

    Plasmid
    #140004
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140004-plasmid-map-sequence-id-275398

    Enlarge

  18. EF1a-TET1-dCas9

    Plasmid
    #235593
    Purpose
    Tet1CD fused to N-terminus of dCas9
    Depositor
    Ting Zhou
    Publication
    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TET1-dCas9 (TET1 Human, SpCas9 is from Streptococcus pyogenes)
    Use
    CRISPR
    Tags
    3xNLS, TET1 CD, and tagBFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    235593-plasmid-map-sequence-id-467826

    Enlarge

Showing: 1621 - 1640 of 2672 results