We narrowed to 13,572 results for: cas9 genes

1. AAV-GfABC1D-SaCas9-WPRE3-pA

   Plasmid

   #203540

   Purpose

   SaCas9 expression in astrocytes

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   SaCas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-SpCas9-2A-GFP-noITR

   Plasmid

   #118415

   Purpose

   Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_UdgX-APOBEC1-UdgX-nCas9

   Plasmid

   #163555

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-dCas9-dMQ1-EGFP

   Plasmid

   #89637

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG, 6*His, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   C141S, TGC-->TCC; S317A, AGC-->GCC

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_UdgX-APOBEC1-UdgX-HF-nCas9

   Plasmid

   #163559

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-HF-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVS1-CAG-hCAS9-neo

   Plasmid

   #166026

   Purpose

   AAVS1 targeting vector carrying CAG-SpCas9-neo

   Depositor

   Kosuke Yusa

   Insert

   AAVS1-CAGCas9IRESneo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. R26-dCas9-VPR-mNG

   Plasmid

   #172441

   Purpose

   Rosa26 targeting vector, expresses dCas9-VPR and mNeonGreen

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Inserts

   dCas9-VPR
   mNeonGreen
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACT1:Cas9-GFP, U6:sgUPRT

   Plasmid

   #122853

   Purpose

   Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   Cas9-GFP
   U6-sgUPRT
   

   Use

   CRISPR; Cas9-gfp plasmid for genome editing of cr…

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Cryptosporidium parvum U6 and Cryptosporidium par…

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti_PGK-Cas9-P2A-HygR

   Plasmid

   #164135

   Purpose

   Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   SpCas9 (cas9 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMCS-rybozyme-IRES-CAS9

    Plasmid

    #64668

    Purpose

    Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

    Depositor

    Wataru Fujii

    Article

    Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

    Inserts

    CAS9
    HDV ribozyme
    

    Use

    CRISPR

    Tags

    Flag, Internal ribosome entry site, and guide RNA…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGG>nls-hCas9-nls

    Plasmid

    #99138

    Purpose

    Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    humanized Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-SpCas9D10A nickase

    Plasmid

    #216737

    Purpose

    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMVd1

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pH-Ubi-miniSdd7-nCas9-PBE

    Plasmid

    #204856

    Purpose

    For cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    ZmUbi-1, OsU3, 35S

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti_CMV-Cas9-P2A-HygR

    Plasmid

    #164133

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKMD106e - intein-Cas9(S219)

    Plasmid

    #64190

    Purpose

    Expresses intein-Cas9(S219) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_RBMX-eA3A-UdgX-HF-nCas9

    Plasmid

    #163548

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-HF-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pED9x (dCas9-KRAB-mCherry)

    Plasmid

    #163956

    Purpose

    Lentiviral expression plasmid of sgRNA with mCherry

    Depositor

    Junwei Shi

    Article

    Giles et al Immunity. 2022 Mar 8;55(3):557-574.e7. d

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

    Plasmid

    #98974

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 3 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-nCas9n-P2A-TagBFP2-pA

    Plasmid

    #176026

    Purpose

    Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nCas9n-P2A-TagBFP2
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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