425 results

1. E. coli MEV20

   Bacterial Strain

   #197113

   Purpose

   E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Bacterial Resistance

   None

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

2. E. coli MEV15

   Bacterial Strain

   #197112

   Purpose

   E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Bacterial Resistance

   None

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

3. V. natriegens NC7

   Bacterial Strain

   #215356

   Purpose

   Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

   Depositor

   Buz Barstow

   Article

   Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

   Bacterial Resistance

   None

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

4. MG1655 ΔendA ΔrecA

   Bacterial Strain

   #37853

   Depositor

   Kristala Prather

   Article

   Leonard et al Proc Natl Acad Sci U S A. 2010 Aug 3;107

   Bacterial Resistance

   None

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

5. sAJM.1504

   Bacterial Strain

   #108251

   Purpose

   Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Bacterial Resistance

   Chloramphenicol

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

6. MG1655 ΔendA ΔrecA (DE3)

   Bacterial Strain

   #37854

   Depositor

   Kristala Prather

   Article

   Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

   Bacterial Resistance

   None

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

7. S2060

   Bacterial Strain

   #105064

   Purpose

   host strain for phage assisted continuous evolution

   Depositor

   David Liu

   Article

   Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

   Bacterial Resistance

   Streptomycin and tetracycline

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

8. sAJM.1506

   Bacterial Strain

   #108254

   Purpose

   Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Bacterial Resistance

   Chloramphenicol

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

9. B95(DE3) ΔA ΔfabR ΔserB

   Bacterial Strain

   #197655

   Purpose

   This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

   Depositor

   Richard Cooley, Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Bacterial Resistance

   None

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

10. BL21 Rosetta2 ΔrecBCD

    Bacterial Strain

    #176583

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

11. C321.ΔA.exp

    Bacterial Strain

    #49018

    Purpose

    This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Bleocin (zeocin)

    Species

    Escherichia coli

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

12. B-95.ΔAΔfabR

    Bacterial Strain

    #197934

    Purpose

    BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

    Bacterial Resistance

    None

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

13. C321.ΔA

    Bacterial Strain

    #48998

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Ampicillin

    Species

    Escherichia coli

    Available Since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

14. B-95.ΔA

    Bacterial Strain

    #197933

    Purpose

    BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

    Bacterial Resistance

    None

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

15. rEcoli XpS

    Bacterial Strain

    #192872

    Purpose

    MG1655-C321 -- All 321 UAG codons changed to UAA

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

    Bacterial Resistance

    Bleocin (zeocin)

    Species

    Escherichia coli

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

16. C321.∆A T7RNAP ompT- rne- lon-

    Bacterial Strain

    #182778

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Spectinomycin

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

17. E. coli K-12 MG1655 RARE

    Bacterial Strain

    #61440

    Purpose

    This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

    Depositor

    Kristala Prather

    Article

    Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

    Bacterial Resistance

    None

    Available Since

    Feb. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

18. RHO3

    Bacterial Strain

    #124700

    Purpose

    E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

    Depositor

    Herbert Schweizer

    Article

    Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

    Bacterial Resistance

    Dap (400 µg/ml)

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

19. ATMW-BL21

    Bacterial Strain

    #218769

    Purpose

    E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

    Bacterial Resistance

    Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

    Species

    Escherichia coli

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

20. C321.deltaA (Isaacs lab)

    Bacterial Strain

    #73581

    Purpose

    Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Bacterial Resistance

    Ampicillin

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only