422 results

1. Syn61Δ3(ev5) ΔrecA (ev1)

   Bacterial Strain

   #189857

   Purpose

   Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

   Depositor

   George Church

   Article

   Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

   Bacterial Resistance

   Streptomycin

   Species

   Synthetic

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

2. B-95.ΔAΔfabR

   Bacterial Strain

   #197934

   Purpose

   BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

   Bacterial Resistance

   None

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

3. BJ5183 cells

   Bacterial Strain

   #16398

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Bacterial Resistance

   Streptomycin

   Species

   Escherichia coli

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

4. B-95.ΔA

   Bacterial Strain

   #197933

   Purpose

   BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

   Bacterial Resistance

   None

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

5. AdEasier-1 cells (strain)

   Bacterial Strain

   #16399

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Bacterial Resistance

   Ampicillin and streptomycin

   Species

   Escherichia coli

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

6. BL21(DE3)-R3-pRARE2

   Bacterial Strain

   #26242

   Purpose

   Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Bacterial Resistance

   Chloramphenicol

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

7. E. coli K-12 MG1655 RARE

   Bacterial Strain

   #61440

   Purpose

   This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

   Depositor

   Kristala Prather

   Article

   Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

   Bacterial Resistance

   None

   Available Since

   Feb. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

8. RF1

   Bacterial Strain

   #61962

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. glyA gene deleted

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

9. RF2

   Bacterial Strain

   #62070

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC gene deleted

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

10. E. coli BL21 (DE3) ΔEntD

    Bacterial Strain

    #192874

    Purpose

    Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

    Depositor

    Christophe Guilhot, Christian Chalut

    Article

    Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

11. S2060

    Bacterial Strain

    #105064

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

    Bacterial Resistance

    Streptomycin and tetracycline

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

12. Escherichia coli DH10B-MOB

    Bacterial Strain

    #229395

    Purpose

    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Bacterial Resistance

    60 ug/ml of dap

    Species

    Escherichia coli

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

13. Syn61

    Bacterial Strain

    #174513

    Purpose

    Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

    Depositor

    Jason W Chin

    Article

    Fredens et al Nature. 2019 May;569(7757):514-518. doi:

    Bacterial Resistance

    Streptomycin

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

14. MG1655 Z1 malE

    Bacterial Strain

    #65915

    Purpose

    E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Bacterial Resistance

    None

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

15. Ochre (rEcΔ2.ΔA.B3.tW*)

    Bacterial Strain

    #234622

    Purpose

    Genomically recoded bacteria for site-specific incorporation of two nonstandard amino acids into proteins

    Depositor

    Farren Isaacs

    Article

    Grome et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Bacterial Resistance

    Hygromycin

    Available Since

    May 16, 2025

    Availability

    Academic Institutions and Nonprofits only

16. C321.ΔA.exp

    Bacterial Strain

    #49018

    Purpose

    This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Bleocin (zeocin)

    Species

    Escherichia coli

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

17. V. natriegens NC7

    Bacterial Strain

    #215356

    Purpose

    Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

    Depositor

    Buz Barstow

    Article

    Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

    Bacterial Resistance

    None

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

18. C321.∆A T7RNAP ompT- rne- lon-

    Bacterial Strain

    #182778

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Spectinomycin

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

19. RHO3

    Bacterial Strain

    #124700

    Purpose

    E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

    Depositor

    Herbert Schweizer

    Article

    Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

    Bacterial Resistance

    Dap (400 µg/ml)

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

20. EC1000

    Bacterial Strain

    #71852

    Purpose

    RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

    Depositor

    Jan Kok, Todd Klaenhammer

    Article

    Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

    Bacterial Resistance

    Kanamycin

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only