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B95(DE3) ΔA ΔfabR ΔserB

Bacterial Strain

#197655

Purpose

This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

Depositor

Ryan Mehl, Richard Cooley

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Bacterial Resistance

None

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecBCD

Bacterial Strain

#176583

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
∆cosσε ∆HG

Bacterial Strain

#197132

Purpose

Deficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Bacterial Resistance

Hygromycin

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only
Escherichia coli DH10B-MOB

Bacterial Strain

#229395

Purpose

Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Bacterial Resistance

60 ug/ml of dap

Species

Escherichia coli

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only
MG1655-OptoCre-knt-P**-R

Bacterial Strain

#188477

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Bacterial Resistance

None

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only
C321.ΔA.exp

Bacterial Strain

#49018

Purpose

This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
RHO3

Bacterial Strain

#124700

Purpose

E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

Depositor

Herbert Schweizer

Article

Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

Bacterial Resistance

Dap (400 µg/ml)

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only
BL21 ΔrecBCD

Bacterial Strain

#176581

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Depositor

Chase Beisel, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

None

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
B-95.ΔAΔfabR

Bacterial Strain

#197934

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only
BL21(DE3)-R3-pRARE2

Bacterial Strain

#26242

Purpose

Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Jun 10. ():.

Bacterial Resistance

Chloramphenicol

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only
E. coli BW25113 ΔtnaA ΔtrpR

Bacterial Strain

#205015

Purpose

Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

Depositor

Yajun Yan

Article

Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

Bacterial Resistance

None

Species

Escherichia coli

Available since

Aug. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits
BL21 Rosetta2 ΔrecBCD GamS

Bacterial Strain

#176586

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol and ampicillin

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
S2060

Bacterial Strain

#105064

Purpose

host strain for phage assisted continuous evolution

Depositor

David Liu

Article

Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

Bacterial Resistance

Streptomycin and tetracycline

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only
LC-E75

Bacterial Strain

#115925

Purpose

Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Bacterial Resistance

None

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only
C321.ΔA

Bacterial Strain

#48998

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Ampicillin

Species

Escherichia coli

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
AdEasier-1 cells (strain)

Bacterial Strain

#16399

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial Resistance

Ampicillin and streptomycin

Species

Escherichia coli

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
B-95.ΔA

Bacterial Strain

#197933

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only
eScaf

Bacterial Strain

#220921

Purpose

cssDNA production strain

Depositor

Shawn Douglas

Article

Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

Bacterial Resistance

None

Species

Escherichia coli

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only
V. natriegens NC7

Bacterial Strain

#215356

Purpose

Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

Depositor

Buz Barstow

Bacterial Resistance

None

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only
rEcoli XpS

Bacterial Strain

#192872

Purpose

MG1655-C321 -- All 321 UAG codons changed to UAA

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 20 of 409 results

Results per page:

Sort results by:

B95(DE3) ΔA ΔfabR ΔserB

BL21 Rosetta2 ΔrecBCD

∆cosσε ∆HG

Escherichia coli DH10B-MOB

MG1655-OptoCre-knt-P**-R

C321.ΔA.exp

RHO3

BL21 ΔrecBCD

B-95.ΔAΔfabR

BL21(DE3)-R3-pRARE2

E. coli BW25113 ΔtnaA ΔtrpR

BL21 Rosetta2 ΔrecBCD GamS

S2060

LC-E75

C321.ΔA

AdEasier-1 cells (strain)

B-95.ΔA

eScaf

V. natriegens NC7

rEcoli XpS

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