421 results

1. BL21(DE3)-R3-pRARE2

   Bacterial Strain

   #26242

   Purpose

   Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Jun 10. ():.

   Bacterial Resistance

   Chloramphenicol

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

2. BL21(DE3) ΔserC

   Bacterial Strain

   #197656

   Purpose

   Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

   Depositor

   Richard Cooley, Ryan Mehl

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Bacterial Resistance

   None

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

3. B-95.ΔA

   Bacterial Strain

   #197933

   Purpose

   BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

   Bacterial Resistance

   None

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

4. JM101

   Bacterial Strain

   #50349

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Messing et al Recombinant DNA Technical Bulletin, 1979

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. HB101 endA::frt lambda pir

   Bacterial Strain

   #45472

   Depositor

   Michele Swanson

   Article

   Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

   Bacterial Resistance

   Streptomycin

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

6. KJ740(DE3)

   Bacterial Strain

   #179486

   Purpose

   KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Bacterial Resistance

   Tetracycline (chromosome, tn10) (10 μg/ml); streptomycin/spectinomycin (chromosome) (50 μg/ml)

   Species

   Escherichia coli

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

7. Syn61Δ3(ev5)

   Bacterial Strain

   #174514

   Purpose

   Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Bacterial Resistance

   Streptomycin

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

8. B95(DE3) ΔA ΔfabR ΔserB

   Bacterial Strain

   #197655

   Purpose

   This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

   Depositor

   Richard Cooley, Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Bacterial Resistance

   None

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

9. C321.ΔA.exp

   Bacterial Strain

   #49018

   Purpose

   This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

   Depositor

   George Church

   Article

   Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

   Bacterial Resistance

   Bleocin (zeocin)

   Species

   Escherichia coli

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Syn61

    Bacterial Strain

    #174513

    Purpose

    Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

    Depositor

    Jason W Chin

    Article

    Fredens et al Nature. 2019 May;569(7757):514-518. doi:

    Bacterial Resistance

    Streptomycin

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

11. C321.∆A T7RNAP ompT- rne- lon-

    Bacterial Strain

    #182778

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Spectinomycin

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

12. BL21 ΔrecBCD

    Bacterial Strain

    #176581

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

    Depositor

    Chase Beisel, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    None

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

13. Strain: C321.ΔA

    Bacterial Strain

    #68306

    Purpose

    Genomically recoded strain of E. coli (no genomic TAG)

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Bacterial Resistance

    Bleocin (zeocin)

    Available since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

14. JM110

    Bacterial Strain

    #49763

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Yanisch-Perron et al Gene. 1985;33(1):103-19.

    Bacterial Resistance

    Streptomycin

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

15. bMS.346

    Bacterial Strain

    #220588

    Purpose

    Increase editing efficiency of retron and oligo recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Bacterial Resistance

    None

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

16. V. natriegens NC7

    Bacterial Strain

    #215356

    Purpose

    Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

    Depositor

    Buz Barstow

    Bacterial Resistance

    None

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

17. S2060

    Bacterial Strain

    #105064

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

    Bacterial Resistance

    Streptomycin and tetracycline

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

18. E. coli K-12 MG1655 RARE

    Bacterial Strain

    #61440

    Purpose

    This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

    Depositor

    Kristala Prather

    Article

    Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

    Bacterial Resistance

    None

    Available since

    Feb. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

19. C321.∆A T7RNAP ompT- rne-

    Bacterial Strain

    #182777

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Spectinomycin

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

20. B-95.ΔAΔfabR

    Bacterial Strain

    #197934

    Purpose

    BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

    Bacterial Resistance

    None

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only