419 results

1. Syn61Δ3(ev5)

   Bacterial Strain

   #174514

   Purpose

   Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Bacterial Resistance

   Streptomycin

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

2. B-95.ΔAΔfabR

   Bacterial Strain

   #197934

   Purpose

   BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

   Depositor

   Kensaku Sakamoto

   Article

   Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

   Bacterial Resistance

   None

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

3. BL21∆serB

   Bacterial Strain

   #34929

   Depositor

   Dieter Söll, Jesse Rinehart

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Bacterial Resistance

   None

   Available since

   Feb. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

4. BL21(DE3)-R3-pRARE2

   Bacterial Strain

   #26242

   Purpose

   Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Jun 10. ():.

   Bacterial Resistance

   Chloramphenicol

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

5. EcNR1 Strain

   Bacterial Strain

   #26930

   Depositor

   George Church

   Article

   Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

   Bacterial Resistance

   Ampicillin

   Available since

   Jan. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

6. C321.∆A T7RNAP ompT- rne- lon-

   Bacterial Strain

   #182778

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Spectinomycin

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

7. S2060

   Bacterial Strain

   #105064

   Purpose

   host strain for phage assisted continuous evolution

   Depositor

   David Liu

   Article

   Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

   Bacterial Resistance

   Streptomycin and tetracycline

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

8. E. coli K-12 MG1655 RARE

   Bacterial Strain

   #61440

   Purpose

   This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

   Depositor

   Kristala Prather

   Article

   Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

   Bacterial Resistance

   None

   Available since

   Feb. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

9. BL21ΔBCF

   Bacterial Strain

   #102269

   Purpose

   lamB, ompC, ompF triple knockout strain for overproduction of outer membrane proteins

   Depositor

   Jack Leo

   Article

   Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

   Bacterial Resistance

   None

   Available since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

10. MG1655 ΔendA ΔrecA (DE3)

    Bacterial Strain

    #37854

    Depositor

    Kristala Prather

    Article

    Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

    Bacterial Resistance

    None

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

11. C321.ΔA.exp

    Bacterial Strain

    #49018

    Purpose

    This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Bleocin (zeocin)

    Species

    Escherichia coli

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

12. B95(DE3) ΔA ΔfabR ΔserB

    Bacterial Strain

    #197655

    Purpose

    This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

    Depositor

    Richard Cooley, Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Bacterial Resistance

    None

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

13. EcNR2 Strain

    Bacterial Strain

    #26931

    Depositor

    George Church

    Article

    Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

14. BL21 ΔrecBCD

    Bacterial Strain

    #176581

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

    Depositor

    Chase Beisel, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    None

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

15. Ochre (rEcΔ2.ΔA.B3.tW*)

    Bacterial Strain

    #234622

    Purpose

    Genomically recoded bacteria for site-specific incorporation of two nonstandard amino acids into proteins

    Depositor

    Farren Isaacs

    Article

    Grome et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Bacterial Resistance

    Hygromycin

    Available since

    May 16, 2025

    Availability

    Academic Institutions and Nonprofits only

16. C321.ΔA

    Bacterial Strain

    #48998

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Ampicillin

    Species

    Escherichia coli

    Available since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

17. B-95.ΔA

    Bacterial Strain

    #197933

    Purpose

    BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

    Bacterial Resistance

    None

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

18. BL21(DE3) ΔserC

    Bacterial Strain

    #197656

    Purpose

    Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

    Depositor

    Richard Cooley, Ryan Mehl

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Bacterial Resistance

    None

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

19. BL21 Rosetta2 ΔrecBCD GamS

    Bacterial Strain

    #176586

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

20. BL21ΔCF

    Bacterial Strain

    #102265

    Purpose

    ompC, ompF double knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only