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27,469 results

Showing: 6061 - 6080 of 27469 results

  2. pX330a dCas9-VP64

    Plasmid
    #92363
    Purpose
    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VP64
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92363-plasmid-map-sequence-id-178232

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  4. pMOD_B2203

    Plasmid
    #91066
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    CmYLCV
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91066-plasmid-map-sequence-id-181864

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  7. pCIneo-hCHD7

    Plasmid
    #89458
    Purpose
    constitutive expression of human CHD7 in mammalian cells
    Depositor
    Peter Scacheri
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CHD7 (CHD7 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89458-plasmid-map-sequence-id-176093

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  8. pSG5-SUV39H2-FL

    Plasmid
    #89135
    Purpose
    mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regions
    Depositor
    Elizabeth Wilson
    Publication
    Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SUV39H2 (SUV39H2 Human)
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89135-plasmid-map-sequence-id-176081

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  12. pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

    Plasmid
    #85072
    Purpose
    Green/red FRET biosensor for RhoA in the GTP locked constitutively active state
    Depositor
    Dorus Gadella
    Publication
    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RhoA Biosensor constitutively active (Q63L) (RHOA Human)
    Tags
    6xHis and mScarlet-i and SGFP2
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Q63L
    Promoter
    CMV
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    85072-plasmid-map-sequence-id-163878

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  16. Lyn11-CIBN-GFP

    Plasmid
    #79572
    Purpose
    expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Publication
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn11
    Tags
    CIBN (CRY2-binding domain) and EGFP
    Expression
    Mammalian
    Mutation
    G8R (please see depositor comments below)
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  17. KRas FAK biosensor

    Plasmid
    #78301
    Purpose
    FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells
    Depositor
    Yingxiao Wang
    Publication
    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRas FAK biosensor
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. Rlucrarr3-Flash5

    Plasmid
    #74133
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.
    Depositor
    Louis Luttrell
    Publication
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Arrestin3
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 6061 - 6080 of 27469 results