Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
100 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
100 µg Transfection-Ready DNA Clear filter

27,467 results

Showing: 6841 - 6860 of 27467 results

  3. EGFP-Rab8a-TN

    Plasmid
    #86077
    Purpose
    A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hRab8A (RAB8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Changed threonine 22 to aspargine
    Promoter
    CMV
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86077-plasmid-map-sequence-id-168966

    Enlarge

  5. pcDNA-mCherry-4A/4B-CAAX

    Plasmid
    #87699
    Purpose
    Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells
    Depositor
    Robert Campbell
    Publication
    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-4A/4B-CAAX
    Expression
    Mammalian
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    87699-plasmid-map-sequence-id-177046

    Enlarge

  6. AAVS1-Pur-CAG-hM3Dq-mCherry

    Plasmid
    #80948
    Purpose
    human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter
    Depositor
    Su-Chun Zhang
    Publication
    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hM3Dq-mCherry
    Tags
    mcherry fusion
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80948-plasmid-map-sequence-id-212456

    Enlarge

  8. pSLI-2xFKBP-GFP(1463000)

    Plasmid
    #85789
    Purpose
    P. falciparum integration plasmid to fuse endogenous genes with tags using SLI
    Depositor
    Tobias Spielmann
    Publication
    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R
    Use
    P. falciparum integration
    Tags
    2xFKBP, GFP
    Promoter
    none
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    85789-plasmid-map-sequence-id-166704

    Enlarge

  9. Fireworks RFP[PTC+] (AVA2626)

    Plasmid
    #85444
    Purpose
    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.
    Depositor
    Joan Steitz
    Publication
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Expression
    Mammalian
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85444-plasmid-map-sequence-id-171527

    Enlarge

  10. p55-H2B-Dendra2

    Plasmid
    #80609
    Purpose
    Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.
    Depositor
    Periklis Pantazis
    Publication
    Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-Dendra2 (H2BC21 Human, Synthetic)
    Tags
    Dendra2
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    80609-plasmid-map-sequence-id-159250

    Enlarge

  11. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

    Plasmid
    #84246
    Purpose
    Expresses direct fusion VPR-Sa dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    VPR-tagBFP-Sa dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84246-plasmid-map-sequence-id-160889

    Enlarge

  12. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid
    #84243
    Purpose
    Expresses ABA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84243-plasmid-map-sequence-id-160884

    Enlarge

  13. pEGFP-Rab21-T31N (DN)

    Plasmid
    #83423
    Purpose
    Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.
    Depositor
    Johanna Ivaska
    Publication
    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab21 (Rab21 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Residue 31 from T to N (T31N: ACG -> AAC)
    Promoter
    CMV
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83423-plasmid-map-sequence-id-159112

    Enlarge

  14. pTol2p2A--3mnx1:mTagBFP-NS-MCS

    Plasmid
    #78250
    Purpose
    Can be used to subclone in gene of interest into blue motor neuron expression vector.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    -3mnx1 (mnx1 Zebrafish)
    -mTagBFP-NS-MCS
    Use
    Zebrafish motor neuron expression
    Promoter
    -3mnx1
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78250-plasmid-map-sequence-id-149784

    Enlarge

  16. pEB1deltaC-mCherry

    Plasmid
    #80603
    Purpose
    Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end
    Depositor
    Torsten Wittmann
    Publication
    Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EB1 (MAPRE1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Truncated C-terminus; EB1 coding sequence ends at…
    Promoter
    CMV
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80603-plasmid-map-sequence-id-203900

    Enlarge

  17. pmCherry-Paxillin

    Plasmid
    #80029
    Purpose
    Mammalian expression of mouse Paxillin
    Depositor
    Chinten James Lim
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Paxillin (Pxn Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80029-plasmid-map-sequence-id-365712

    Enlarge

  20. mCherry-CRY2-INPP5E

    Plasmid
    #79561
    Purpose
    expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Publication
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    INPP5E (INPP5E Human)
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 6841 - 6860 of 27467 results