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30,870 results

Showing: 3261 - 3280 of 30870 results

  3. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid
    #140005
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140005-plasmid-map-sequence-id-275488

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  4. Dead-Laconic/pcDNA3.1(-)

    Plasmid
    #118627
    Purpose
    To express in mammalian cells a lactate FRET sensor negative control.
    Depositor
    Luis Felipe Barros
    Publication
    Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dead-Laconic
    Expression
    Mammalian
    Mutation
    Change Histidine 151 to Aspartic Acid of Escheric…
    Promoter
    CMV Promoter
    Available since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118627-plasmid-map-sequence-id-231761

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  5. pc DNA FRT TO- APPSwed/Ind

    Plasmid
    #114194
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Expression
    Mammalian
    Mutation
    Popular mouse model, overexpress 2 mutations: Swe…
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114194-plasmid-map-sequence-id-224256

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  6. pC1-SypHer3s-IMS

    Plasmid
    #108120
    Purpose
    Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.
    Depositor
    Vsevolod Belousov
    Publication
    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SypHer3s-IMS
    Tags
    ratiometricYFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108120-plasmid-map-sequence-id-262557

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  10. pGL4-ERSE2-luc2P-Hygro

    Plasmid
    #101790
    Purpose
    Luciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)
    Depositor
    Seiichi Oyadomari
    Publication
    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xERSE-II
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Promoter
    minimal TATA-box promoter with low basal activity
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101790-plasmid-map-sequence-id-194148

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  12. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

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  15. pAAVS1-P-CAG-GFPluc2

    Plasmid
    #80493
    Purpose
    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression
    Depositor
    Knut Woltjen
    Publication
    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFPluc2
    Use
    Donor vector (human)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80493-plasmid-map-sequence-id-151598

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  18. PR_GEF YFP (2XPDZ-YFP-LARG DH)

    Plasmid
    #80408
    Purpose
    Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells
    Depositor
    Michael Glotzer
    Publication
    Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)
    Tags
    YFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80408-plasmid-map-sequence-id-151385

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  19. pCDNA3-STAT3-S727D

    Plasmid
    #73364
    Purpose
    Expression of STAT3-S727D
    Depositor
    Jie Chen
    Publication
    Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
    Tags
    FLAG tagged
    Expression
    Mammalian
    Mutation
    E16K S727D
    Promoter
    CMV
    Available since
    May 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73364-plasmid-map-sequence-id-423259

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Showing: 3261 - 3280 of 30870 results