30,870 results
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Plasmid#110823PurposePiggyBac compatible plasmid expressing dCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9
ExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS6-YFP-SMASh(AI)
Plasmid#111500PurposeExpresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYFP-SMASh(AI)
TagsHA epitope tag and SMASh tagExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23
Plasmid#111726PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ23 (polyQ repeat)PromoterCMV and P10Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-ChroME-mRuby2-ST
Plasmid#108902PurposeCation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertChroME-mRuby2-ST
ExpressionMammalianPromoterCAGAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA4-GFP-Vinculin
Plasmid#107153Purposemammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVinculin (VCL Human)
Tags(His)10-GFPExpressionMammalianPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 IGF1R-BirA(R118G)-HA
Plasmid#109232PurposeThis construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIGF1R (IGF1R Human)
TagsBirA(R118G) (highly promiscuous form) and HAExpressionMammalianPromoterT7Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-dCpf1-eBE
Plasmid#107688PurposeExpresses dLbCpf1-eBE in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdLbCpf1-eBE
ExpressionMammalianMutationdLbCpf1(D832A/E925A/D1148A)PromoterCMVAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 asyn E46K
Plasmid#105730PurposeMammalian expression of mutant human alpha synucleinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha-synuclein E46K (SNCA Human)
ExpressionMammalianMutationE46KPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA6 asyn A30P
Plasmid#105729PurposeMammalian expression of mutant human alpha synucleinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha-synuclein A30P (SNCA Human)
ExpressionMammalianMutationA30PPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hygro(+)-mscGAS
Plasmid#102607PurposeMammalian expression of mouse cGASDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcGAS (Cgas Mouse)
ExpressionMammalianPromoterCMVAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS Donor mClover-LMNA
Plasmid#98970PurposeDonor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination ReporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
ExpressionMammalianAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro
Plasmid#89992PurposeDonor template for generation of OCT4-T2A-nEmGFP reporter cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
UseCRISPRPromoternoneAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUGI-NLS
Plasmid#101091PurposeExpresses UGI in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUGI
TagsNLSExpressionMammalianPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLSSmOrange-mKate2
Plasmid#99868PurposeMeasure FRET between LSSmOrange and mKate2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLSSmOrange
TagsmKate2ExpressionMammalianPromoterCMVAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-NLS-PhoCl-mCherry
Plasmid#87691PurposeExpress NLS-PhoCl-mCherry in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-PhoCl-mCherry
Tagsnuclear localization signalExpressionMammalianAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)
Plasmid#86886PurposeN-terminal part of the split-BioIDDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPP1g - FLAG - BirA (AA2-140)
TagsFLAG tagExpressionMammalianMutationBirA Amino Acids 2-140Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ILID-CAAX
Plasmid#85680PurposeExpression of untagged ILID CAAXDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertILID-CAAX
ExpressionMammalianPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPaint-TagRFP-PuroR
Plasmid#80971PurposeCRISPaint gene taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagRFP
UseGene taggingPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Gβ-2A-cpV-Gγ2-IRES-Gαi2-mTq2
Plasmid#69624PurposeA FRET sensor for Galphai2 activation, encoded on a single plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsGbeta-2A-cpV-Ggamma2
Galphai2-mTurquoise2
Tagsciruclar permutated Venus (cp173V) and mTurquoise…ExpressionMammalianPromoterCMV and CMV (IRES)Available sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eBFP2 (eBFP2)_CD
Plasmid#66034PurposeMoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFluorescent reporter - BFP
UseSynthetic BiologyAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only