32,662 results
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Plasmid#229261PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RSF1010_PartType1DepositorInsertRSF1010 Origin
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYC-yhaM-RFP
Plasmid#207651PurposeOverexpression of native L-cysteine desulfidase and RFP in E. coli MG1655DepositorInsertyhaM
Tags6xHisExpressionBacterialPromoterT7Available SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-dCas9
Plasmid#220193Purposemini-Tn7 dCas9 expression vectorDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterpromoterlessAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046749
Plasmid#145629PurposeBacterial Expression plasmid for SARS-CoV-2 nsp11DepositorInsertSARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcI-ts,ind+-(NSP8)
Plasmid#160656PurposeExpresses codon optimized SARS-CoV-2 NSP8 in E. coliDepositorInsertSARS-CoV-2 NSP8 (ORF1ab Synthetic)
ExpressionBacterialAvailable SinceDec. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
TRBV5-1
Plasmid#123414Purposeconstruction of TCRDepositorAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS mTSP-2
Plasmid#12999DepositorInsertTSP-2 (Thbs2 Mouse)
ExpressionBacterialAvailable SinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SpG-His
Plasmid#179318PurposePlasmid for bacterial expression and purification of SpG-Cas9DepositorInsertHuman codon-optimized Cas9 variant SpG
UseCRISPRTags6xHis and SV40-NLSExpressionBacterialMutationSpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337RAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-p53 (1-90) (72R)
Plasmid#62081PurposeExpression of human p53 (TAD residues 1-90) (72R) in e. coliDepositorInsertp53 (TP53 Human)
TagsGSTExpressionBacterialMutationContains TAD/PP residues 1-90, 72RPromotertacAvailable SinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-HiBiTGFPHis
Plasmid#188317PurposeExpression of HiBiT-GFP via T7 promoter in TXTLDepositorInsertHiBiT-GFP fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialPromoterT7MaxAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SurA
Plasmid#213138PurposeExpresses chaperone SurADepositorAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC234
Plasmid#216357PurposexhNup93-Nb3t (+2Cys) Nanobody productionDepositorInsertxhNup93-Nb3t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP2844
Plasmid#160675PurposeMobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
PAJ698
Plasmid#178947PurposeExpresses P2 tail fiber (gpH) in fusion with S16 tail fiber (gp37)DepositorInsertChimeric Tail Fiber Fusion P2-gpH and S16 gp37
UseUnspecifiedExpressionBacterialAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB1-moxCerulean3
Plasmid#103975PurposePlasmid encoding moxCerulean3DepositorInsertmoxCerulean3
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-recAmu-sacB
Plasmid#158717PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.DepositorInsertrecA
ExpressionBacterialMutationR61CAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKD233.7-3
Plasmid#90955PurposeExpresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutivelyDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
ExpressionBacterialPromoterBba_J23114, PltetO-1, and PttrB185-269Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYDE009
Plasmid#194151PurposeDelivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.DepositorInserttetR-tetP-dcas9-rrnB T1-T7Te
ExpressionBacterialPromotertetpAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a asyn(Δ41-54/Δ103-130)
Plasmid#242236PurposeExpresses alpha synuclein (missing exon 3 and 5, residues 41-54 and 103-130) in bacteriaDepositorInsertasyn(Δ41-54/Δ103-130)
TagsnoneExpressionBacterialMutationdeleted amino acids 41-54 and 103-130Available SinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21a asyn(Δ103-130)
Plasmid#242235PurposeExpresses alpha synuclein (missing exon 5, residues 103-130) in bacteriaDepositorInsertasyn(Δ103-130)
TagsnoneExpressionBacterialMutationdeletion of residues 103-130Available SinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21a asyn(Δ41-54)
Plasmid#242234PurposeExpresses alpha synuclein (missing exon 3, residues 41-54) in bacteriaDepositorInsertasyn(Δ41-54)
TagsnoneExpressionBacterialMutationdeleted amino acids 41-54Available SinceAug. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-His-sfGFP
Plasmid#180754PurposepET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
Tagsstrep tagExpressionBacterialMutationG140C- Please see depositor commentsAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIGc-Xph20
Plasmid#133024PurposeExpresses Xph20 in bacteriaDepositorInsertXph20
TagsHis10ExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNP1 eforRed trigger
Plasmid#107357PurposeTrigger sequence for pNP1 eforRed toehold switchDepositorInserttoehold trigger 1
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPBAD-yhjH
Plasmid#111613PurposeArabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjHDepositorInsertyhjH
ExpressionBacterialMutationA52VAvailable SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG020 (AVAST type 4)
Plasmid#157898PurposeExpresses the AVAST type 4 defense systemDepositorInsertAVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
ExpressionBacterialAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG019 (AVAST type 3)
Plasmid#157897PurposeExpresses the AVAST type 3 defense systemDepositorInsertAVAST type 3 (STAND ATPase with N-terminal DUF4297)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Zn2
Plasmid#174805PurposeBacterial expression of isolated PARP1 Zn2 domainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Zn3
Plasmid#174806PurposeBacterial expression of isolated PARP1 Zn3 domainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV4Neo-murine IL-17RA-HA
Plasmid#46859Purposeexpresses mouse IL-17RADepositorAvailable SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-Smad3 WT
Plasmid#27010DepositorAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
RGS2
Plasmid#38812PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 1-320
Plasmid#85998Purposebacterial expression of his6 and SUMO tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
TagsSUMOExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pPSU60
Plasmid#173833PurposeExpresses 60 kD Penn State ladder proteinDepositorInsertHSTPABPACRCC1
ExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delBRCT
Plasmid#173938PurposeBacterial expression of PARP1 lacking BRCT domainDepositorInsertPARP1delBRCT (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 374-520; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G38R)-Strep
Plasmid#196209PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
TagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG38RAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-araC
Plasmid#62563PurposeArabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGST-DHFR Mm
Plasmid#83073PurposeExpresses the full length DHFR Mm in E.coliDepositorAvailable SinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_UBA52_Ubiquitin
Plasmid#110043PurposeProtein expression and purification of UBA52_UbiquitinDepositorInsertUBA52_Ubiquitin (UBA52 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBHM2619
Plasmid#234498PurposeamdS-CnU6-empty sgRNADepositorInsertamdS-PCnU6-sgRNA_scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2662
Plasmid#234501PurposeLinker-Cno_mNeonGreen-CBP-2xFLAG-CnU6-sgRNA-amdSDepositorInsertmNeonGreen-CBP-2XFLAG-amdS-PCnU6-sgRNA_scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
PCMV-Tag4A-MMP8 (E138Q)
Plasmid#29549DepositorInsertMetalloproteinase protein 8 (MMP8 Human)
TagsFLAGExpressionBacterial and MammalianMutationE138QAvailable SinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBEL1092
Plasmid#195635PurposeExpression/purification of 6xHis-TEV-sfGFPDepositorInsertsfGFP
ExpressionBacterialAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS_TXS
Plasmid#163839PurposeiPTG inducible expression of taxadiene synthase and geranylgeranyl diphosphate synthaseDepositorInserttaxadiene synthase (61-862)
ExpressionBacterialPromoterTrcAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176854PurposeExpresses membrane bound biotin ligase, BioID2, in neuronsDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromoterhSyn1Available SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFN18A-HaloTag-SpyCatcher
Plasmid#206041PurposeHaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTagDepositorTypeEmpty backboneTagsHaloTag and SpyCatcher-8HisExpressionBacterialPromoterT7Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPING
Plasmid#26046DepositorInsertSS peptide-LacZ-KHHHHHH
TagsKHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETGExpressionBacterial, Insect, and Mamm…Available SinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only